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Home > Products > ELISArray Kits > Single Analytes > Mouse IL13 Single Analyte ELISArray Kit SEM03021A
Mouse IL13 Single Analyte ELISArray Kit SEM03021A |
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| Description |
The Mouse IL13 Single Analyte ELISArray Kit
is designed to quantitatively measure the amount of this cytokine in cell
culture supernatant, serum or plasma using a conventional enzyme-linked
immunosorbent assay (ELISA). Each 96-well plate (8 wells/strip, 12 strips)
is coated with the protein-specific capture antibody. We screened all
commercially available antibodies to identify the best capture and
detection antibodies. The high sensitivity, good linearity and low
background of the Single Analyte ELISArray Kits provide reliable and
reproducible results for your cytokine and chemokine analyses.
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| Product Resources |
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| More information on IL13 from NCBI: |
| "This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4." |
| Kit Contents / Storage Conditions/
Shelf Life |
| Please check the kit components immediately after you receive this package. SABiosciences is only responsible for missing items reported within two (2) business days of receipt.
Enough reagents are provided to process the included plate of 12 ELISA
strips.
| Component / Description |
Quantity |
| BOX 1: Shipped on blue ice packs. Store
at -20 ºC. |
| Antigen Standard (1 µg/ml) |
1.5-ml tube |
| Detection Antibody |
1.5-ml tube |
| Avidin-HRP Conjugate |
1.5-ml tube |
| 10% BSA |
15 ml bottle |
| Donkey Serum |
15 ml bottle |
| BOX 2: Shipped at ambient temperature.
Store at 4 ºC. |
| Pre-coated Capture Antibody 8-well strips |
One plate of 12 strips in a pouch |
| Sample Dilution Buffer Stock |
60 ml bottle |
| Assay Buffer Stock |
60 ml bottle |
| Wash Buffer (10 × Concentrate) |
125 ml bottle |
| Development Solution |
25 ml bottle |
| Stop Solution |
60 ml bottle |
Shelf Life: Do not use kit beyond the expiration date printed on the label. |
| Brief Protocol |
The Brief Protocol is meant for experience
users only. First-time users should refer to the User
Manual.
- Prepare replicate serial dilutions of the Antigen Standard and your
experimental samples.
- Pipette 50 µl of Assay Buffer into each well of the 8-well ELISA
strips.
- Transfer 50 µl samples and/or standards to the appropriate wells of
the ELISA strips.
- Gently shake or tap plate for 10 seconds. Incubate for 2 hours at room
temperature.
- Washing ELISA Wells:
Decant or aspirate well contents. Add 350 µl 1 × Washing Buffer.
Gently shake or tap plate for 10 seconds. Decant or aspirate. Blot array
upside down on absorbent paper to remove any residual buffer. Repeat
wash twice more.
- Pipette 100 µl of Detection Antibody solution. Incubate 1 hour at room
temperature.
- Wash ELISA wells as described above.
- Add 100 µl Avidin-HRP solution to all wells. Incubate for 30 minutes at
room temperature.
- Wash ELISA wells for a total of 4 washes.
- Add 100 µl of Development Solution to each well. Incubate the plate
for 15 minutes at room temperature in the dark.
- Add 100 µl of Stop Solution to each well. The color changes from
blue to yellow.
- Read absorbance at 450 nm within 30 minutes of stopping the reaction. If
wavelength correction is available, subtract readings at 570 nm from
the reading at 450 nm.
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