siRNA

shRNA

SIR345251ABCD Set of Four Designs (12.5 nmol each) Individual Design SIR345251A Design A (12.5 nmol/tube) SIR345251B Design B (12.5 nmol/tube) SIR345251C Design C (12.5 nmol/tube) SIR345251D Design D (12.5 nmol/tube)

Gene Information

Description:    Gene Symbol: UniGene #: Refseq Accession #: The RefSeq Accession number refers to the representative sequence used to design the enclosed siRNA.

Description

SureSilencing siRNA is HPLC purified synthetic siRNA with >97% purity, which ensures its high specificity, high potency and high efficacy. SureSilencing siRNA is available for specifically knockdown the expression of every human, mouse or rat gene by RNA interference under transient transfection conditions. The availability of at least two effective siRNA designs, guaranteed to knockdown the expression of target gene, allows you to control for non-specific and off-target effects. Guarentee: At least two of the four provided pre-designed SureSilencing™ siRNA are guaranteed to knock down expression of the targeted gene at the RNA level by at least 70 percent as measured by real-time qRT-PCR in transfected cells as described in this User Manual. SureSilencing siRNA comes with a one-time-only replacement offer. If a set of SureSilencing siRNAs is ordered and less than 2 of the siRNAs result in gene silencing, SABiosciences will provide 2 additional siRNAs free of charge, once only. You will be asked to provide supporting data, demonstrating that the siRNA failed to knock down the target gene by at least 70% at the mRNA level under appropriate transfection conditions. Supporting data should include transfection efficiency data, quantitative silencing data, and data showing ≥ 70% knockdown of a positive control. This offer is valid for up to 6 months after the date of delivery.

Useful Links

Pricing and Ordering User Manual White Paper Negative Control siRNA qPCR Primer siRNA Transfection Reagent

Protocol

Resuspension Instruction Resuspend 12.5 nmol lyophilized siRNA duplex in 625 µl RNase-free ddH2O to get a 20 µM stock solution. Aliquots are recommended when repeated use is needed. Transfection Instruction First time users, please refer to the complete protocol in the SureSilencing™ siRNA User Manual. For 96-well transfection, on the day of transfection, dilute SureFECT 0.1 - 0.3 µl to 37.7 - 37.9 µl Opti-MEM I medium for each well. Mix 2-6 pmol (2µl) of siRNA and the negative control siRNA into 38ul of Opti-MEM™ I diluted SureFECT. Mix gently. Incubate siRNA-SureFECT™ mix for 20 min at room temperature. Add 160 ul cells suspension (prepared during the incubation) at 8000 - 12000 cells/160µl in regular culture media to each well of 96-well plate. Mix gently. Incubate the cells at 37 ºC in a CO2 incubator for 24 to 72 hours. Determine the target gene knock down usually at 48 hours after transfection with real-time PCR. Determine the phenotype change at the time point you optimized with proper reagent and/or equipments.

Materials Included

Please check the kit components immediately after you receive this package. SABiosciences is only responsible for missing items reported within two (2) business days of receipt. Kit Contents: Component Specification Quantity siRNA SureSilencing siRNA, 12.5nmol in each tube  Four (4) for Set One (1) for individual siRNA InfoSheet SureSilencing siRNA InfoSheet with siRNA sequences  One (1) Storage Conditions: Store siRNA duplex (either lyophilized or resuspended) at -20º C or below in non-frost-free freezer, avoid contamination of RNases and repeated freeze-thaw cycles. Stable for at least 6 months from the date of shipment.