The APX System is the most advanced technology for adjusting expression levels of exogenous proteins. The APX System provides two levels of flexibility for the scientist. Each protein is available as either a constitutively-expressed protein or an adjustable fusion protein. The adjustable proteins are fusion proteins that contain the wild-type coding sequence fused to a destabilization domain. The fusion protein’s stability is regulated by the presence of a small membrane-permeable molecule, APX Centry Stabilization Reagent, in a dose-dependent fashion. Finally, expression constructs are available in either a ready-to-transfect plasmid format or as ready-to-transduce lentiviral particles.

Human Proteins

Mouse Proteins

Cloning Vectors & Controls

Oct4 Name Fused Peptide Product Expression Cat. No. Oct4-DD Destablization Domain Plasmid Adjustable DB5-101A Oct4 DYKDDDDK* Plasmid Constitutive DA2-101A Oct4-DD Lenti Destablization Domain Lentivirus Adjustable DO4-101A Oct4 Lenti DYKDDDDK* Lentivirus Constitutive DN1-101A c-Myc Name Fused Peptide Product Expression Cat. No. c-Myc-DD Destablization Domain Plasmid Adjustable DB6-102A c-Myc DYKDDDDK* Plasmid Constitutive DA3-102A c-Myc-DD Lenti Destablization Domain Lentivirus Adjustable DO5-102A c-Myc Lenti DYKDDDDK* Lentivirus Constitutive DN2-102A Nanog Name Fused Peptide Product Expression Cat. No. Nanog-DD Destablization Domain Plasmid Adjustable DB8-104A Nanog DYKDDDDK* Plasmid Constitutive DA5-104A Nanog-DD Lenti Destablization Domain Lentivirus Adjustable DO7-104A Nanog Lenti DYKDDDDK* Lentivirus Constitutive DN4-104A KLF4 Name Fused Peptide Product Expression Cat. No. KLF4-DD Destablization Domain Plasmid Adjustable DB7-103A KLF4 DYKDDDDK* Plasmid Constitutive DA4-103A KLF4-DD Lenti Destablization Domain Lentivirus Adjustable DO6-103A KLF4 Lenti DYKDDDDK* Lentivirus Constitutive DN3-103A Sox2 Name Fused Peptide Product Expression Cat. No. Sox2-DD Destablization Domain Plasmid Adjustable DB9-105A Sox2 DYKDDDDK* Plasmid Constitutive DA6-105A Sox2-DD Lenti Destablization Domain Lentivirus Adjustable DO8-105A Sox2 Lenti DYKDDDDK* Lentivirus Constitutive DN5-105A LIN28 Name Fused Peptide Product Expression Cat. No. LIN28-DD Destablization Domain Plasmid Adjustable DB0-106A LIN28 DYKDDDDK* Plasmid Constitutive DA7-106A LIN28-DD Lenti Destablization Domain Lentivirus Adjustable DO9-106A LIN28 Lenti DYKDDDDK* Lentivirus Constitutive DN6-106A * The DYKDDDDK epitope is also referred to as the FLAG® epitope. Oct4 Name Fused Peptide Product Expression Cat. No. Oct4-DD Destablization Domain Plasmid Adjustable DB0-601A Oct4 DYKDDDDK* Plasmid Constitutive DA7-601A Oct4-DD Lenti Destablization Domain Lentivirus Adjustable DO9-601A Oct4 Lenti DYKDDDDK* Lentivirus Constitutive DN6-601A c-Myc Name Fused Peptide Product Expression Cat. No. c-Myc-DD Destablization Domain Plasmid Adjustable DB1-602A c-Myc DYKDDDDK* Plasmid Constitutive DA8-602A c-Myc-DD Lenti Destablization Domain Lentivirus Adjustable DO0-602A c-Myc Lenti DYKDDDDK* Lentivirus Constitutive DN7-602A Nanog Name Fused Peptide Product Expression Cat. No. Nanog-DD Destablization Domain Plasmid Adjustable DB3-604A Nanog DYKDDDDK* Plasmid Constitutive DA0-604A Nanog-DD Lenti Destablization Domain Lentivirus Adjustable DO2-604A Nanog Lenti DYKDDDDK* Lentivirus Constitutive DN9-604A KLF4 Name Fused Peptide Product Expression Cat. No. KLF4-DD Destablization Domain Plasmid Adjustable DB2-603A KLF4 DYKDDDDK* Plasmid Constitutive DA9-603A KLF4-DD Lenti Destablization Domain Lentivirus Adjustable DO1-603A KLF4 Lenti DYKDDDDK* Lentivirus Constitutive DN8-603A Sox2 Name Fused Peptide Product Expression Cat. No. Sox2-DD Destablization Domain Plasmid Adjustable DB4-605A Sox2 DYKDDDDK* Plasmid Constitutive DA1-605A Sox2-DD Lenti Destablization Domain Lentivirus Adjustable DO3-605A Sox2 Lenti DYKDDDDK* Lentivirus Constitutive DN0-605A LIN28 Name Fused Peptide Product Expression Cat. No. LIN28-DD Destablization Domain Plasmid Adjustable DB5-606A LIN28 DYKDDDDK* Plasmid Constitutive DA2-606A LIN28-DD Lenti Destablization Domain Lentivirus Adjustable DO4-606A LIN28 Lenti DYKDDDDK* Lentivirus Constitutive DN1-606A * The DYKDDDDK epitope is also referred to as the FLAG® epitope. Expression Vectors Name Fused Peptide Product Expression Cat. No. Empty Vector -DD Destablization Domain Plasmid Adjustable DB3-000A Empty Vector DYKDDDDK* Plasmid Constitutive DA0-000A GFP Name Fused Peptide Product Expression Cat. No. GFP-DD Destablization Domain Plasmid Adjustable DB4-001A GFP-DD Lenti Destablization Domain Lentivirus Adjustable DO3-001A Centry DD-Protein Stabilization Reagent Name Cat. No. APX Centry Reagent SA-03