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Chromatin IP (ChIP) and qPCR Assay System
ChampionChIP™ System provides a complete platform for the in vivo analysis of the
dynamic interaction between DNA and nuclear proteins that play a central role in
gene regulation. This system provides an easy and fast One-Day ChIP kit, high
quality Antibody Kits, specifically designed and validated genome-wide qPCR
primers, and pathway- or disease- focused ChIP PCR Arrays, for a comprehensive
analysis of genomic sequences associated with transcription factors and
co-regulators, modified and unmodified histones, and other DNA-binding proteins.
If you are looking for a reliable and convenient Chromatin IP method, you've
found it. How It Works
How it works
The ChIP PCR array is a set of optimized real-time PCR primer assays on
96-well or 384-well plates for pathway or disease focused analysis of in vivo
protein-DNA interactions. The ChIP PCR array performs ChIP DNA analysis with
real-time PCR sensitivity and the multi-genomic loci profiling capability of a
ChIP-on-chip. Simply mix your ChIP DNA samples with the appropriate ready-to-use
PCR master mix, aliquot equal volumes to each well of the same plate, and then
run the real-time PCR cycling program.
Benefits
- Reliable & Consistent: Get high-quality results every time
- Easy & Fast: Ready-to-use and results obtained within one or two days
- Complete Solution: Streamlined system from Chromatin IP to qPCR analysis
for one region of interest or multiple loci simultaneously.
ChampionChIP™ PCR Array
Simultaneous analysis of multiple genomic sites bound to proteins with
Real-Time PCR precision. The reliability and simplicity of the ChIP Array make
it an ideal tool for routine use in any research laboratory.
Custom ChampionChIP™ PCR Array
Custom-made PCR arrays for analysis of a panel of genomic loci of your choice
ChampionChIP™ One-Day Kit
The entire protocol can be easily done in 6 hours, with high yield of
high-quality ChIP DNA sufficient for 100-200 qPCR assays.
ChampionChIP™ Antibody Kits
Includes high quality experimentally validated ChIP-grade antibody of interest,
control IgG, and both positive and negative control PCR primer sets for insured
antibody specificity and efficiency.
ChampionChIP™ PCR Primers
Experimentally validated genome-wide qPCR primers specially designed for the
analysis of ChIP enriched genomic DNA.
ChIP PCR Data Analysis
Free Excel-based conversion of your raw Ct values into easy to use readouts of
percentage of input and fold difference.
ChampionChIP™ Search Portal
Free easy-to-use Web tool to find potential regulators of your gene of interest
and PCR primers for the binding sites of those transcription factors
RT² SYBR™ Green PCR Master Mixes
Ready-to-use and validated for ChIP DNA analysis
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