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Home > Products > ChIP-qPCR > ChampionChIP™ ChIP-Grade Antibody Kits
RNA Polymerase II ChIP-Grade Antibody Kit, Human
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How It Works: Speed and Ease
Immunoprecipitation Done in 2 Hours with ChampionChIP Antibody Using
an optimized IP buffer system provided by the ChampionChIP One-Day Kit, the
efficiency and specificity of the ChIP-grade antibody are maximized such that
immunoprecicipation can be done within 2 hours. We guaranteed high
signal-to-noise ratio and low background when the Champion Antibody kit is used
in combination of ChampionChIP One-Day Kit. In addition, these antibody kits are
the first ones in the market that include positive and negative control qPCR
primers, insuring the accuracy of ChIP analysis. The figure depicts the workflow
of the entire ChampionChIP System including One-Day ChIP Kit, ChIP-Grade
Antibody Kit, and FREE Excel-based data analysis template.
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Performance: Signal-to-Noise Ratios
Detecting RNA Polymerase II Enrichment on GAPD Promoter
The ChampionChIP System Detects RNA Polymerase II Enrichment at a
Housekeeping Gene Promoter with Expected Signal-to-Noise Ratios.
Triplicate wells of HCT-116 cells were fixed with formaldehyde. Chromatin
was isolated and sonicated in preparation for immunoprecipitation using the
Human RNA Polymerase II Antibody Kit and the One-Day Kit. The resulting
enriched genomic DNA was then was purified and characterized by real-time
PCR with primers for the proximal promoter region of the GAPD promoter and
for an ORF-free region as a negative control. The results for each
immunoprecipitation are expressed as percent of input, or the fraction of
the total input DNA co-immunoprecipitating with RNA Polymerase II.
The immunoprecipitation (IP) with the RNA Polymerase II antibody (RNA
Pol II) brings down much more GAPD proximal promoter than the IP with control
IgG. Neither source
of antibody precipitates a significant amount of a specific genomic DNA
sequence within an ORF-free intergenic region or "promoter
desert" lacking any known or predicted structural genes.
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ChampionChIP™ One-Day Kits
A simple and robust ChIP kit. The entire protocol can be easily completed in 6 hours. The high yield of high-quality ChIP DNA is free of inhibitors and ready for real-time PCR quantification.
ChampionChIP™ PCR Primers
Genome-wide qPCR primers specifically designed for analyzing genomic DNA
enrichment from ChIP. The specificity and high amplification efficiencies are
validated and guaranteed.
ChampionChIP™ Customized PCR Array
Simultaneous analysis of multiple regulatory sites bound by transcription
factors, co-regulators or modified histones. The two available array formats
allow you to study:
- Association of your transcription factors of interest with their binding
sites on multiple genes of interest
- Association of histone modification and chromatin remodelling with gene
regulation across a 30-kb tiling region (-20 kb to +10 kb) around the TSS of
any gene
RT² SYBR Green qPCR Mixes
Optimized for the instrument in your lab by the experts in high-performance SYBR
Green quantitative PCR
ChampionChIP™ qPCR Data Analysis Excel File
Excel-based template. Simply paste in your data, and results are automatically
reported.
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Please check the kit components immediately after you receive this
package. We are not responsible for any missing items not reported within two
(2) business days upon receipt.
| GAH-111: Human RNA Polymerase II Antibody Kit |
| Tube ID |
Component |
| E111 |
Anti-Human RNA Polymerase II Antibody (200 µg/mL) |
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mIgG |
Mouse Control IgG (400 µg/mL) |
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GPH10001C(+)01A |
Human GAPDH ChIP-qPCR Control Primers |
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GPH00001C(-)01A |
Human IGX ChIP-qPCR Negative Control Primers |
Storage Conditions
ChampionChIP™ Antibody Kits are shipped with ice packs.
For long-term storage, keep the entire kit at 4 ºC.
Shelf Life
All reagents are stable for 6 months after receipt of the kit if stored at
the recommended temperature.
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