Di-Methylated Histone H3 at Lysine 4 (H3K4me2) ChIP-Grade Antibody Kit, Human

Human

Mouse

ChampionChIP™ ChIP-Grade Antibody Kit (H3K4me2) Catalog No. Contents Price GAH-3203 Antibody & Control IgG (24 µg each), Sufficient for 12 Paired Chromatin Immunoprecipitations Three Sets of Control qPCR Primers, Each Sufficient for 200 25-µL Reactions. $ 349 The ChampionChIP Modified Histone Antibody Kits provide the specific antibodies and real-time PCR positive and negative control primers needed for a complete and reliable analysis of the histone modifications that regulate chromatin structure and accessibility. Its modified histone-specific ChIP-grade antibody has been rigorously validated for efficient and specific enrichment of DNA representative of either transcriptionally active euchromatin, or transcriptionally inactive euchromatin, or heterochromatin using a panel of real-time PCR primers. The matched control IgG provided in the kit has also been extensively tested for minimal co-immunoprecipitation of genomic DNA. The control qPCR primers detecting DNA representative of different chromatin states are included for the appropriate positive and negative controls to insure the efficiency and specificity of each ChIP sample preparation. When used with our ChampionChIP One-Day Kit, the antibody kit yields the highest signal-to-noise ratios and the lowest backgrounds. Stop tediously screening your own ChIP-grade antibodies and primers and stop optimizing your own ChIP conditions. Let the ChampionChIP Modified Histone Antibody Kits give you the complete solution for reliable ChIP-based characterization of histone modifications. Learn More Histone modifications are critical for regulating chromatin structure and function, which in turn affect transcription, recombination, DNA repair and replication, and chromosome organization1. Di-methylated histone H3 at lysine 4 (H3K4me2) is enriched at regions surrounding known transcriptional start sites, and is generally associated with transcriptional activation2-4. References: Kouzarides T., Cell, 128, 693-705 (2007). The ENCODE Project Consortium, Nature, 447, 799-816 (2007). Koch, C. M., et al, Genome Res, 17, 691-707 (2007). Barski, A., Cell, 129, 823-37 (2007).

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How It Works: Speed and Ease Immunoprecipitation Done in 2 Hours with ChampionChIP Antibody Using an optimized IP buffer system provided by the ChampionChIP One-Day Kit, the efficiency and specificity of the ChIP-grade antibody are maximized such that immunoprecicipation can be done within 2 hours. We guaranteed high signal-to-noise ratio and low background when the Champion Antibody kit is used in combination of ChampionChIP One-Day Kit. In addition, these antibody kits are the first ones in the market that include positive and negative control qPCR primers, insuring the accuracy of ChIP analysis. The figure depicts the workflow of the entire ChampionChIP System including One-Day ChIP Kit, ChIP-Grade Antibody Kit, and FREE Excel-based data analysis template.

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Sonicated chromatin from HeLa cells (20 µg) was immunoprecipitated with 2 µg of anti-H3K4me2 antibody (blue bars) or control IgG (red bars) for 2 h using the ChampionChIP One-Day Kit (GA-101). The resulting input and ChIP DNA fractions were characterized with qPCR primers specific for the GAPDH, MYOD1 and SAT2 genomic loci to calculate the percentage of co-precipitating DNA relative to input. The immunoprecipitation using anti-H3K4me2 enriched genomic DNA representing actively transcribed euchromatin (the GAPDH locus) with a high signal-to-noise ratio, demonstrating the efficiency of the Antibody and One-Day ChIP Kit combination. In contrast, the immunoprecipitation did not significantly enrich genomic DNA representing either transciptionally inactive euchromatin (the MYOD1 locus) or heterochromatin (the SAT2 locus) relative to the immunoprecipitation using control IgG, demonstrating the specificity of the Antibody and One-Day ChIP Kit combination. Back to Top

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ChampionChIP™ One-Day Kits A simple and robust ChIP kit. The entire protocol can be easily completed in 6 hours. The high yield of high-quality ChIP DNA is free of inhibitors and ready for real-time PCR quantification. ChampionChIP™ PCR Primers Genome-wide qPCR primers specifically designed for analyzing genomic DNA enrichment from ChIP. The specificity and high amplification efficiencies are validated and guaranteed. ChampionChIP™ Customized PCR Array Simultaneous analysis of multiple regulatory sites bound by transcription factors, co-regulators or modified histones. The two available array formats allow you to study: Association of your transcription factors of interest with their binding sites on multiple genes of interest Association of histone modification and chromatin remodelling with gene regulation across a 30-kb tiling region (-20 kb to +10 kb) around the TSS of any gene RT² SYBR Green qPCR Mixes Optimized for the instrument in your lab by the experts in high-performance SYBR Green quantitative PCR ChampionChIP™ qPCR Data Analysis Excel File Excel-based template. Simply paste in your data, and results are automatically reported. Back to Top

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Please check the kit components immediately after you receive this package. We are not responsible for any missing items not reported within two (2) business days upon receipt. Tube ID  Part Number Component Anti-H3K4me2 Ab, Human N/A Anti-Di-Methylated Histone H3 at Lysine 4 Antibody (1 µg/µL) rIgG N/A Rabbit Control IgG (1 mg/mL) GAPDH GPH110001C(+)01A Human GAPDH ChIP-qPCR Control Primers for Transcriptionally Active Euchromatin MYOD1 GPH110002C(+)01A Human MYOD1 ChIP-qPCR Control Primers for Transcriptionally Inactive Euchromatin SAT2 GPH110003C(+)01A Human SAT2 ChIP-qPCR Control Primers for Heterochromatin Storage Conditions ChampionChIP™ Antibody Kits are shipped with ice packs. For long-term storage, keep the entire kit at 4 ºC. Shelf Life All reagents are stable for 6 months after receipt of the kit if stored at the recommended temperature. Back to Top