Transcription Factor Researchers:
Use this search portal to find PCR primers for your favorite transcription
factors' binding sites if you know their location relative to the TSS of your
genes of interest.
This search portal returns all 30 available primers tiled around the TSS
of the queried gene.
Use the drop-down button to select the tile closest to your binding site
and see the relative position of the primers.
Because primer designs fall anywhere within a specified 1-kb tile region,
the closest assay to your binding site of interest may actually lie in an
adjacent tile. Contact technical support with any questions.
Histone Modification Researchers:
Use this search portal to identify PCR primers for specific regions in your
favorite gene bound by modified histones. For example:
The primers for the +1 and -1 kb tiles are often used to define two major
groups of histone modifications related to transcriptional activation and
repression of most genes.
The primers for all thirty tiles in a gene's promoter may be used in a Custom
Promoter Binding PCR Array to discover the binding positions of
different modified histones across that gene's promoter.