Custom ChampionChIP PCR Arrays allow you to define your own gene list, and we
will make Customized ChIP PCR Arrays tailored to your specific research
interest. You can take advantage of our proprietary bioinformatic algorithms
that design ChIP appropriate primers for analyzing the in vivo binding
of transcription factors, modified and unmodified histones and other nuclear
proteins up to 384 genomic loci in a few hours.
Our rigorous qPCR primer validation and quality control manufacture process
guarantee reliable assay performance. Build your Custom PCR Array using our
genome-wide ChIP-qPCR primer assays.
Available Custom ChIP PCR Arrays:
Regulatory Elements Array
To validate ChIP-on-chip and ChIP-seq results
Transcription Factor Binding Sites Array
To screen the binding sites of the transcription factors of your interest on a
panel of genes
30-kb Tiling Array
To identify the binding of transcription factors, histones, modified histones
and other nuclear protein to a specific gene
* Additional format available - please inquire.
* Inquire for high volume order (larger than 24 PCR Arrays).
How to Order
Determine the content of your Custom PCR Array. Check online database to
find the catalogued primers for your genes/genomic loci.
Select a plate format to match the number of genes or genomic loci you
would like to analyze. Fill your gene list with catalogue numbers into an
Excel file.
Determine the number of plates required.
Call Technical Support at 1-888-503-3187 or email support@sabiosciences.com
to communicate with your account manager who will assist you in completing
your Custom PCR Array order. Please have the type of Real-Time PCR instrument
available when placing the order.
Email your Account Manager the filled Customer Array Excel file with your
gene list as an attached Excel file.
Upon receiving the Excel file with your gene list, your Account Manager
will contact you to confirm your Custom PCR Array design and to provide a
price quote as well.
Custom PCR Arrays will be shipped in 2-3 weeks from order receipt.
Signaling pathway activation of transcription factors (TFs) can occur by
a variety of mechanisms. Anti-cancer agents, such as 5-fluorouracil (5FU),
have been shown to induce apoptosis by activation of TFs such as the tumor
suppressor protein, p53. The p53 protein activation induces a site-specific
binding event that allows coordination of p53 interactions with chromatin
and transcriptional complexes at the promoter region of a target gene.
These interactions are often cell-type and tissue-specific. ChampionChIP
PCR Arrays were used to examine the cell type-specific p53 occupancy before
and after 5FU treatment, on the promoter region of a panel of genes
implicated in apoptosis and cell cycle regulation. Three cancer cell lines
with wild-type p53 protein (A549, HepG2, MCF7 cells) and with functional
mutant p53 protein (PC3 cells) were tested.
Figure 1:
A Model for Stimuli-Specific
Regulation or Tissue/Cell Type-Specific Response to Drug Treatment.
Figure 2:
Association between Transcription
Binding Site Occupancy and Differential Expression. Four different cell
lines treated and untreated with 5FU were subjected to either; ChIP
assay using ChampionChIP
One-Day Kit and ChampionChIP
p53 Antibody Kit or gene expression analysis using RT²
p53 Signaling Pathway PCR Arrays. The results from both types of
Arrays were represented as the fold change upon 5FU treatment.
Comparison of the fold changes in both gene expression levels and
p53-binding site occupancy implies a coordinated cell type-specific and
stimuli-specific gene regulation by p53. The results show that the ChIP PCR
Arrays is a powerful tool in studying complex pathway signals affecting
gene transcription.
Characteristic Patterns of Histone Modification
Custom ChampionChIP PCR Arrays can be used to monitor
differential histone modifications across 30Kb surrounding transcription
start site of a gene.
Figure 3:
The Custom ChampionChIP PCR
Array Quickly Maps Histone Modifications Surrounding the Transcription
Start Site (TSS) of CDKN1A Gene. ChampionChIP Antibodies against
modified histones (H3Ac, H3K4me2, H3K27me3), or Control (NIS) were used
to precipitate chromatin from one million HeLa cells. Each ChIP DNA
fraction was analyzed with a ChampionChIP Tiling Array representing 30
one-kb tile intervals across the genomic sequence of the CDKN1A gene.
The results indicate the enrichment of histone marks for actively
transcribed genes (H3Ac and H3K4me2) but not markers for transcriptional
inactive genes (H3K27me3) in the genomic region surrounding the TSS of
CDNK1A.
