Custom ChIP-qPCR Primers

If the 1 kb tiling of annotated promoters in our cataloged ChIP-qPCR Primers does not meet your detection needs, we can design custom primers to other genomic DNA sequences relevant to your experiments. The Custom ChIP-qPCR Primers are designed with the same stringent bioinformatic algorithms and validated with the same rigorous quality control process used for our cataloged primers. And like the cataloged primers, the Custom ChIP-qPCR Primers are guaranteed to work under the same qPCR conditions permitting multiple primers to be performed in the same qPCR run. Let us design ChIP-qPCR Primers for your genomic DNA regions of interest.

Applications for Custom ChIP-qPCR Primers:

Whole Genome or Whole Chromosome Tiling Microarray Validation
Our Custom ChIP-qPCR Primer design can accommodate the validation of ChIP-on-chip results from whole genome or whole chromosome tiling microarrays that fall outside of SABiosciences' cataloged 30-kb tiling range. Simply provide us with the Chromosome RefSeq positions identified by your microarray results. We will then design ChIP-qPCR Primers to detect and validate ChIP enrichment at those positions.

Non-Promoter Regions: Chromatin Remodeling or Differentially Methylated Loci
Custom ChIP-qPCR Primer design also accommodates other non-promoter focused ChIP or methylation analyses. For example, monitor chromatin remodeling and differential histone modification (such as acetylation) utilizing anti-histone antibodies during ChIP. Or, monitor differential cytosine methylation at loci of interest using anti-5-methyl-cytosine ChIP or even the MB-PCR or Mira methods of methylated DNA preparations for PCR.

Wider or Narrower Tiling Intervals
If you prefer a tiling scheme for the NCBI-annotated promoters different from our cataloged products' one-kb interval over a 30-kb range, the Custom ChIP-qPCR Primer design can also help you. We can decrease the tiling interval from the cataloged one-kb for greater resolution, or we can go beyond the 30-kb tiling range to help you query more distant enhancer binding elements.

Other Species' Genomes
While SABiosciences' cataloged ChIP-qPCR Primers are currently only available for human, mouse and rat, our Custom ChIP-qPCR Primer design can also be applied to other eukaryotic and prokaryotic genomes, such as Saccharomyces cerevisiae and Drosophila melanogaster.

For more information about our Custom ChIP-qPCR Primers, contact one of our Application Specialists by phone at 888-503-3187 or by email at support@sabiosciences.com.