RT² Hydrolysis Probe Real-Time PCR Assays

 

PCR Arrays

miRNA PCR Arrays

SYBR Single Gene Assays

Probe Single Gene Assays

SABiosciences service core offers comprehensive gene expression services based on hydrolysis probe technology. Probe-based assays have the advantage of virtually no background signal because an internal probe monitors product accumulation. SABiosciences' validated procedures and optimized processes yield the highest data quality and rapid turn-around time. For FFPE (formalin-fixed paraffin-embedded) samples, LCM (laser capture microdissection), FNAB (fine needle aspiration biopsies) and other small samples, require special isolation procedures and may require pre-amplification.

Sample Processing

Data Delivery

Pricing

Submitted Samples are Cells or Tissue:      Option of RNA isolation service      Recommended Sample Isolation and Storage Procedure Submitted Samples are Total RNA:      Recommended RNA Isolation methods      Quantification and estimating RNA purity using a NanoDrop spectrophotometer      Assessing RNA quality using an Agilent 2100 Bioanalyzer with a RNA 6000 NanoChip Reverse-transcription using our RT² First Strand Kit Real-time detection using SABiosciences-designed primers and hydrolysis probes Data analysis Data report delivered via email or secure FTP site Back to Top

Sample Processing

Data Delivery

Pricing

Data analysis is performed using our PCR array data analysis software. Data in an electronic form can be downloaded from a secure FTP website or delivered via email. A complete data and experiment report includes: Raw Ct values ΔΔCt Analysis Excel spreadsheet Custom data format service is also available. Back to Top

Sample Processing

Data Delivery

Pricing

Assay set-up fee: $150 per gene Reverse Transcription: $50 per reaction and include the minus-RT control. Real-Time Probe-based PCR Expression by ΔΔCt: $50 per sample per gene Pre-Amplification: $175 per gene per sample Initial Set Up and Consultation: $300 per project Contact a Technical Support representative to order or to discuss your specific need at support@sabiosciences.com or 301-682-9200. Prices include the cost of material and reagents. Service prices are volume-dependent. Progressive discounts for fees and services are available. All prices are in US Dollars. Prices are subject to change at any time without notice. Back to Top