Custom Human DNA Methylation qPCR Primers & PCR Arrays

Validate Correlations between DNA Methylation Patterns and Phenotypes
Have you found genomic DNA sequences whose methylation status correlates with biological phenotypes that you study? Would you like to quickly and easily validate these associations with a larger number of samples? Custom DNA Methylation qPCR Primers can help!

Correlate DNA Methylation with the Regulated Expression of Any Gene
Have you seen that the expression of specific genes of interest turns off or on under your experimental conditions? Would you like to quickly and easily analyze whether promoter DNA methylation controls those observed changes? Custom DNA Methylation qPCR Primers can help!

Custom Human DNA Methylation qPCR Primer Design
Our Custom DNA Methylation qPCR Primer Service designs real-time primers surrounding 1) your methylated sequences or 2) the CpG islands closest to the promoters of any gene or 3) any other human genomic DNA sequence of interest. Primers are designed to take advantage of our Methyl-Profiler DNA Methylation PCR System, a faster and easier technology for detecting DNA methylation than bisulfite-based methods.

Methyl-Profiler DNA Methylation PCR System
Simply prepare your DNA samples using our DNA Methylation Enzyme Kit to set up four simultaneous digests using methylation-sensitive and/or methylation-dependent restriction enzymes. Then, characterize each digest with the custom designed primers and real-time PCR. A very easy Excel-based data analysis template will calculate the percentage of unmethylated and hypermethylated DNA defining the methylation status of the queried sequences. Now, you can relate that methylation status to the biological phenotypes or regulated gene expression that you study.

Quality Control
All custom primers are first designed by a rigorously optimized computer algorithm that accounts for the GC-rich sequences abundant in genomic DNA and particularly in CpG islands. The design algorithm also insures that every amplicon contains at least two sites for both restriction enzymes for the best possible sensitivity for all methylated fractions. But, we don't just stop there. Before shipment, each primer pair is also experimentally validated on the bench for specificity, by dissociation curve and agarose gel analysis, and for consistently high amplification efficiencies for the most accurate results.

Custom Human DNA Methylation PCR Arrays
These primers can then also be conveniently organized into 96-well or 384-well plates. The need to characterize four restriction digests to analyze the different methylated fractions means that up to 24 or 96 genes or sequences per sample may be characterized on these plate types, respectively. Multiple plates can then characterize multiple DNA samples.

To find out more about our Custom Human DNA Methylation qPCR Primers and PCR Arrays, simply contact us to discuss your sequences or genes of interest and the number of DNA samples that you would like to process.