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Methyl-Profiler™ DNA Methylation PCR Data Analysis

These integrated Excel-based templates automatically perform all ΔCt based calculations from your raw threshold cycle value data to determine gene- or sequence-specific DNA methylation status. Simply paste in the data and gene list from the array used in your experiment. The Excel template normalizes the Ct values of both digests with the mock digestion values to calculate and report the percentage of the queried DNA that is hypermethylated, intermediately methylated, and unmethylated. The completeness of the restriction digests and therefore the quality and reliability of the data is also assessed. The Excel file delivers results not only in a tabular format annotated to the gene list, but also in a graphical chart with gene-specific bars tri-colored for the three different extents of methylation. Use the results to relate the genes' methylation status to the biological phenotypes or regulated gene expression that you study. Make your DNA Methylation analysis as quick and painless as possible with the Methyl-Profiler DNA Methylation PCR System and its Excel-based data analysis.

Benefits:

  • Ease of Use: Simply paste in your data and gene list.
  • Convenience: Results automatically calculated, analyzed, and annotated.

Find the Correct Template for Your Array:

  1. Identify the number genes and the plate type from the Product Specification Sheet.
  2. Click the appropriate link in the table below.
  3. Download and save the correct Excel-file template.
Gene Panel (#) Plate Type (Format) File Download
Signature (24) 96-Well (A, C, D, F) Analyze Data
Signature (24) 384-Well (E, G) Analyze Data
Complete (96) 96-Well (A, C, D, F) Analyze Data
Complete (96) 384-Well (E, G) Analyze Data
  Gene Tables
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