DNA Methylation Related Products

Complement your PCR-based DNA methylation analysis with other experiments using our pathway-focused and genome-wide gene expression and/or gene function analysis solutions. Confirm whether changes in the methylation status of genes affect their expression using real-time RT-PCR. Or explore the function of the methylated genes of interest with RNA interference and cell-based functional assays.

RT² Profiler™ PCR Arrays & RT?qPCR Primers
Verify that hypermethylating or hypomethylating your gene promoters of interest decreases or increases their transcript levels, respectively, and/or alters the expression of other genes in the same biological pathway using real-time RT-PCR. The RT?qPCR Primers analyze the transcript levels of individual genes, while the RT?Profiler PCR Arrays profile a panel of pathway-focused or disease-specific mRNAs. Both methods are optimized for high performance SYBR Green based qRT-PCR detection.

Service Core
Get gene expression profiling results even more quickly and easily by letting our in-house experts perform the real-time RT-PCR or PCR Array analysis for you. Stay focused on your methylation analyses by leaving your mRNA measurements to the experts.

SureSilencing™ shRNA Plasmids & siRNA Arrays
Determine the oncogenic role played by your hypermethylated genes of interest, or genes in the same of related pathways, by knocking down their expression in a normal cell culture model. The SureSilencing shRNA Plasmids are designed to knock down the expression of any gene in the human, mouse, or rat genome by plasmid-based RNA Interference. The SureSilencing siRNA Arrays allow you to knock down the expression of 42 genes in the same biological pathway.

Cignal™ & Cignal Lenti Reporter Arrays & Assays
Confirm that changes in the methylation patterns signal transduction pathway genes alter their ability to be activated in a normal cell culture model. Use Cignal or Cignal Lenti GFP or luciferase based reporter assays to study the regulation of these cellular signaling pathways at the level of transcription factor activation. 

ELISArray™ Kits, Single- & Multi-Analyte
If your hypermethylated or hypomethylated genes of interest encode for cytokines or chemokines, verify their changes in expression at the protein level using standard sandwich ELISA kits.