|Sheared Salmon Sperm DNA is highly purified and optimized for use
during the pre-hybridization and hybridization stages of DNA microarray
analyses to minimize background and non-specific signals and to increase
signal to noise ratios. The solution is provided at a concentration of 10
mg/ml with DNA fragment sizes ranging between 500 and 2000 bp. A 1:100
dilution (0.1 mg/ml final concentration) is recommended for preparing
pre-hybridization and hybridization solutions.
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Please check the kit components immediately after you receive this
package. SABiosciences is not responsible for any missing items not reported
within two (2) business days upon receipt.
Kit Contents: Five tubes of Sheared Salmon Sperm DNA, each
containing a 1-ml solution.
This product is shipped on dry ice.
Store this product at -20 ºC in a manual defrost freezer.
Avoid the repeated freezing and thawing of the same tube.
Prepare multiple aliquots of smaller volumes if necessary.
A. For use with SABiosciences nylon GEArray® Q and S Series Kits:
B. For use with other microarray systems:
Follow the instructions provided in the GEArray® Q and S Series
Kits User Manuals for all steps in this protocol.
Boil the Sheared Salmon Sperm DNA at 100 ºC for 5 minutes and
immediately chill on ice.
Freshly prepare only as much GEAprehyb as needed each day (3 ml per nylon
Add the Sheared Salmon Sperm DNA to GEAhyb Hybridization Solution
(Catalog Number GEA9905100 or H-01) at a final concentration of 0.1
mg/ml. Mix well before starting the pre-hybridization.
Background signal will be minimal if the probe synthesis reaction,
hybridization, washing and detection steps were performed correctly.
Follow the recommendations provided by the microarray manufacturer for
using sheared salmon sperm DNA.