| Description |
| Sheared Salmon Sperm DNA is highly purified and optimized for use
during the pre-hybridization and hybridization stages of DNA microarray
analyses to minimize background and non-specific signals and to increase
signal to noise ratios. The solution is provided at a concentration of 10
mg/ml with DNA fragment sizes ranging between 500 and 2000 bp. A 1:100
dilution (0.1 mg/ml final concentration) is recommended for preparing
pre-hybridization and hybridization solutions.
Need more information about GEArray products? please visit Microarray Home or send Email to Technical Support |
| Packing List/Storage
Conditions |
|
Please check the kit components immediately after you receive this
package. SABiosciences is not responsible for any missing items not reported
within two (2) business days upon receipt.
Kit Contents: Five tubes of Sheared Salmon Sperm DNA, each
containing a 1-ml solution.
Storage Conditions:
This product is shipped on dry ice.
Store this product at -20 ºC in a manual defrost freezer.
Avoid the repeated freezing and thawing of the same tube.
Prepare multiple aliquots of smaller volumes if necessary.
|
| Brief Protocol |
A. For use with SABiosciences nylon GEArray® Q and S Series Kits:
-
Follow the instructions provided in the GEArray® Q and S Series
Kits User Manuals for all steps in this protocol.
-
Boil the Sheared Salmon Sperm DNA at 100 ºC for 5 minutes and
immediately chill on ice.
-
Freshly prepare only as much GEAprehyb as needed each day (3 ml per nylon
GEArray®).
-
Add the Sheared Salmon Sperm DNA to GEAhyb Hybridization Solution
(Catalog Number GEA9905100 or H-01) at a final concentration of 0.1
mg/ml. Mix well before starting the pre-hybridization.
-
Background signal will be minimal if the probe synthesis reaction,
hybridization, washing and detection steps were performed correctly.
B. For use with other microarray systems:
Follow the recommendations provided by the microarray manufacturer for
using sheared salmon sperm DNA.
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