GEArray® Q Series Human Hypoxia Signaling Pathway Gene Array: HS-032
  
Description
GEArray® Q Series Human Hypoxia Signaling Pathway Gene Array contains 96 genes whose expression is affected by hypoxia. The wide variety of such target genes is provided in the list below. Hypoxia is defined as the insufficient supply or utilization of oxygen. Hypoxia alters gene expression through transcriptional activation and post-transcriptional mRNA stabilization mechanisms. Hypoxia activates its specific transcriptional pathway via stabilization of the hypoxia-inducible factor 1 (HIF1) and its subsequent binding to a defined hypoxia response element in the promoter of the affected genes. In additional, HIF-independent pathways are also involved in transcriptional activation by hypoxia. Hypoxia is involved in many physiological and pathophysiological processes such as erythropoiesis, ventilatory adjustment, hypoxic pulmonary vasoconstriction and hypertension, sleep apnea, vascular remodeling, angiogenesis and tumor growth, myocardial and brain ischemic/reperfusion damage. Through a simple side-by-side hybridization using your experimental RNA samples and the arrays and reagents provided in the kit, you can simultaneously determine the expression profile of these 96 hypoxia-regulated genes in your model system.

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Transcription: DR1, FOS (c-fos), HIF1A, HTATIP (cPLA2), LENG4 (BB1).

Survival: BIRC5 (survivin), IL6ST (gp130).

Stress Response: ADM, AZU1, DNAJB1 (Hsp40), ECE1, ENO1, IL1A, IL1B, IL6, IL8, MYBL2, PDIP, PTX3, TST.

Signal Transduction: AGTPBP1, ARD1, CAMK2G, GNA11, GSTTLp28, IQGAP1, NOS2A (iNOS), PPP2CB, PSME2, SAE1.

Protein Synthesis and Turnover: CSTB, EEF1A1, FNBP3 (HYPA), KHSRP, MARS, PSMB3, RPL28, RPL32, RPL37, RPS2, RPS7, RPS8, SNRP70, VARS2.

ECM: MMP14, PLOD3 (lysyl hydroxylase 3).

Cytoskeleton: ADD1 (Adducin 1a), DCTN2 (dynamitin), TUBA1.

Cell growth: CDC42, SSSCA1 (p27).

Biosynthesis: AGPAT2, MAN2B1, MOCS3, NUDT2 (APAH1), SORD, SPTBN1.

Apoptosis: DAPK3 (ZIP-kinase).

Cell cycle: SMT3H2.

E-C coupling: HUMMLC2B.

Metabolism: CA1, CA9, CA12, CHGA, CTGF, EGFR, EPO, GAP43, GAPD, GPI, GPX1, HK2, HMOX1, ID2, IGF2, IGFBP1, KIT (c-kit), LCT, LEP (leptin), LIPE, MYCN, NOTCH1, NPY, RARA, SLC2A1 (GLUT-1), SLC2A4 (GLUT-4), TH, UCP2, VEGF.

Other Related Genes: BAX, BCL2, BHLHB2, CAT, COL1A1, PLAU, PPARA, SOD2.
Storage Conditions
Please check the kit components immediately after you receive this package. SABiosciences is only responsible for missing items reported within two (2) business days of receipt.

GEArray microarrays are shipped at ambient temperature enclosed in either a HybTube or ExpressPak Storage Box. They should be stored at -20 ºC upon receipt.

References
  1. Jiang C, Lu H, Vincent KA, Shankara S, Belanger AJ, Cheng SH, Akita GY, Kelly RA, Goldberg MA, Gregory RJ. Gene expression profiles in human cardiac cells subjected to hypoxia or expressing a hybrid form of HIF-1 alpha. Physiol Genomics. 2002 Feb 11;8(1) : 23-32.
  2. Grosfeld A, Zilberfarb V, Turban S, Andre J, Guerre-Millo M, Issad T. A. Grosfeld et al.: Effects of hypoxia on human PAZ6 adipocytes. Diabetologia. 2002 Apr; 45 (4) : 527-30.
  3. Chauvet C, Bois-Joyeux B, Danan JL. Retinoic acid receptor-related orphan receptor (ROR) alpha4 is the predominant isoform of the nuclear receptor RORalpha in the liver and is up-regulated by hypoxia in HepG2 human hepatoma cells. Biochem J. 2002 Jun 1; 364 (Pt 2): 449-56.
  4. Jogi A, Ora I, Nilsson H, Lindeheim A, Makino Y, Poellinger L, Axelson H, Pahlman S. Hypoxia alters gene expression in human neuroblastoma cells toward an immature and neural crest-like phenotype. Proc Natl Acad Sci U S A. 2002 May 14; 99(10) : 7021-6.
  5. Jelkmann W, Hellwig-Burgel T. Biology of erythropoietin. Adv Exp Med Biol. 2001; 502 : 169-87.
  6. Graven KK, Molvar C, Roncarati JS, Klahn BD, Lowrey S, Farber HW. Identification of protein disulfide isomerase as an endothelial hypoxic stress protein. Am J Physiol Lung Cell Mol Physiol. 2002 May; 282 (5) : L996-1003.
  7. Nishi H, Nishi KH, Johnson AC. Early Growth Response-1 gene mediates up-regulation of epidermal growth factor receptor expression during hypoxia. Cancer Res. 2002 Feb 1; 62 (3) : 827-34.
  8. Lee SY, Madan A, Furuta GT, Colgan SP, Sibley E. Lactase gene transcription is activated in response to hypoxia in intestinal epithelial cells. Mol Genet Metab. 2002 Jan; 75 (1) : 65-9.
  9. Besnard S, Heymes C, Merval R, Rodriguez M, Galizzi JP, Boutin JA, Mariani J, Tedgui A. Expression and regulation of the nuclear receptor RORalpha in human vascular cells. FEBS Lett. 2002 Jan 30; 511(1-3) : 36-40.
  10. Ulleras E, Wilcock A, Miller SJ, Franklin GC. The sequential activation and repression of the human PDGF-B gene during chronic hypoxia reveals antagonistic roles for the depletion of oxygen and glucose. Growth Factors. 2001; 19 (4) : 233-45.
  11. Sato M, Tanaka T, Maeno T, Sando Y, Suga T, Maeno Y, Sato H, Nagai R, Kurabayashi M. Inducible expression of endothelial PAS domain protein-1 by hypoxia in human lung adenocarcinoma A549 cells. Role of Src family kinases-dependent pathway. Am J Respir Cell Mol Biol. 2002 Jan; 26 (1) : 127-34.
  12. Falanga V, Zhou L, Yufit T. Low oxygen tension stimulates collagen synthesis and COL1A1 transcription through the action of TGF-beta1. J Cell Physiol. 2002 Apr; 191 (1) : 42-50.
  13. Yoon DY, Buchler P, Saarikoski ST, Hines OJ, Reber HA, Hankinson O. Identification of genes differentially induced by hypoxia in pancreatic cancer cells. Biochem Biophys Res Commun. 2001 Nov 9; 288 (4) : 882-6.
  14. Shimo T, Kubota S, Kondo S, Nakanishi T, Sasaki A, Mese H, Matsumura T, Takigawa M. Connective tissue growth factor as a major angiogenic agent that is induced by hypoxia in a human breast cancer cell line. Cancer Lett. 2001 Dec 10; 174 (1) : 57-64.
  15. Xu L, Xie K, Mukaida N, Matsushima K, Fidler IJ. Hypoxia-induced elevation in interleukin-8 expression by human ovarian carcinoma cells. Cancer Res. 1999 Nov 15; 59 (22) : 5822-9.
  16. Cooper AL, Beasley D. Hypoxia stimulates proliferation and interleukin-1alpha production in human vascular smooth muscle cells. Am J Physiol. 1999 Oct; 277 (4 Pt 2) : H1326-37.
  17. Gupta M, Mungai PT, Goldwasser E. A new transacting factor that modulates hypoxia-induced expression of the erythropoietin gene. Blood. 2000 Jul 15; 96 (2) : 491-7.
  18. Hoffmann A, Gloe T, Pohl U. Hypoxia-induced upregulation of eNOS gene expression is redox-sensitive: a comparison between hypoxia and inhibitors of cell metabolism. J Cell Physiol. 2001 Jul; 188 (1) : 33-44.
  19. Tazuke SI, Mazure NM, Sugawara J, Carland G, Faessen GH, Suen LF, Irwin JC, Powell DR, Giaccia AJ, Giudice LC. Hypoxia stimulates insulin-like growth factor binding protein 1 (IGFBP-1) gene expression in HepG2 cells: a possible model for IGFBP-1 expression in fetal hypoxia. Proc Natl Acad Sci U S A. 1998 Aug 18; 95 (17) : 10188-93.
  20. Panchenko MV, Farber HW, Korn JH. Induction of heme oxygenase-1 by hypoxia and free radicals in human dermal fibroblasts. Am J Physiol Cell Physiol. 2000 Jan; 278 (1) : C92-C101.
  21. Shi Q, Abbruzzese JL, Huang S, Fidler IJ, Xiong Q, Xie K. Constitutive and inducible interleukin 8 expression by hypoxia and acidosis renders human pancreatic cancer cells more tumorigenic and metastatic. Clin Cancer Res. 1999 Nov; 5 (11) : 3711-21.
  22. Ogita T, Hashimoto E, Yamasaki M, Nakaoka T, Matsuoka R, Kira Y, Fujita T. Hypoxic induction of adrenomedullin in cultured human umbilical vein endothelial cells. J Hypertens. 2001 Mar; 19 (3 Pt 2) : 603-8.
  23. Ivanov S, Liao SY, Ivanova A, Danilkovitch-Miagkova A, Tarasova N, Weirich G, Merrill MJ, Proescholdt MA, Oldfield EH, Lee J, Zavada J, Waheed A, Sly W, Lerman MI, Stanbridge EJ. Expression of hypoxia-inducible cell-surface transmembrane carbonic anhydrases in human cancer. Am J Pathol. 2001 Mar; 158 (3) : 905-19.
  24. Wykoff CC, Beasley NJ, Watson PH, Turner KJ, Pastorek J, Sibtain A, Wilson GD, Turley H, Talks KL, Maxwell PH, Pugh CW, Ratcliffe PJ, Harris AL. Hypoxia-inducible expression of tumor-associated carbonic anhydrases. Cancer Res. 2000 Dec 15; 60 (24) : 7075-83.
  25. Graham CH, Fitzpatrick TE, McCrae KR. Hypoxia stimulates urokinase receptor expression through a heme protein-dependent pathway. Blood. 1998 May 1; 91 (9) : 3300-7.
  26. Piacentini L, Karliner JS. Altered gene expression during hypoxia and reoxygenation of the heart. Pharmacol Ther. 1999 Jul; 83 (1) : 21-37.
  27. Mathupala SP, Rempel A, Pedersen PL. Glucose catabolism in cancer cells: identification and characterization of a marked activation response of the type II hexokinase gene to hypoxic conditions. J Biol Chem. 2001 Nov 6; 276 (46) : 43407-12.
  28. Narravula S, Colgan SP. Hypoxia-inducible factor 1-mediated inhibition of peroxisome proliferator-activated receptor alpha expression during hypoxia. J Immunol. 2001 Jun 15; 166 (12): 7543-8.
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