Functional Gene Grouping

Permissibility and vessel tone:

Angiotensin System: Ace, Ace2, Agt, Agtr1 (AT1), Agtr2 (AT2), Enpep (aminopeptidase A).
NO System: Nos2 (iNOS), Nos3 (eNOS).
Prostacyclin System: Alox5, LOC292645 (PLA2G4C), Ptgis, Ptgs2 (COX-2).
Endothelin System: Edn1 (ET-1), Edn2 (ET-2), EDN3 (ET-3), Ednra (ETA), Ednrb (ETB).
Superoxide Anion System: Ptgs2, Sod1, Xdh.

Angiogenesis:

Adhesion Molecules: Cdh5-M, Itga5, Itgav-M, Itgb3.
Growth Factors and Receptors: Agpt2, Angpt1, Fgf2, Flt1, Flt4, Kdr, Tek, Tgfb1, Tgfb2, Vegf.
ECM: LOC300339 (Mmp1a), Mmp2, Mmp9, Mmp14 (MT1-MMP), Timp1.
Growth Inhibitors: Chga (vasostatin), Col18a1 (endostatin), Plg (angiostatin).

Endothelial cell activation:

Adhesion Molecules Expression: Cdh5-M, Icam1, LOC360647 (Icam2), Itga5, Itgav-M, Itgb1, Itgb3, Ocln (Occludin), Sele (E-Selectin), Sell (L-Selectin), Selp (P-Selectin), Vcam1.
Transition from Antithrombosis to Prothrombosis: Anxa5 (annexin V), Cpb2 (TAFI), F3 (tissue factor), Plat (tPA), Plau (u-PA), Serpine1 (PAI), Tfpi, Tfpi2, Thbd, Vwf.
Cytokine Production: Ccl2, Ccl5, Csf2 (GM-CSF), Csf3 (G-CSF), Cx3cl1, Cxcl2, Cxcl11, Gro1, Il1b, Il3, Il6, Il7, Il11, Il15, Scya20 (Ccl20), Tnf.

Endothelial cell injury (apoptosis):

Pro-Apoptosic Factors: Adam17 (TNFcE), Bax, Casp1, Casp3, Casp6, Casp8, Cradd-M, Mmp9, LOC365454 (Fas), Tnfsf6 (FasL), Tsfsf10 (Trail).
Apoptosis Inhibitors: Bcl2, Bcl2a1, Bcl2l1 (Bcl-xl), Birc1 (NAIP), Birc2 (c-IAP-1), Birc4 (XIAP), Birc5 (Survivin), Cflar (c-FLIP), Fn1, Ptgs2, Timp1, Tnfrsf10b-M, Tnfrsf11b (OPG).

Storage Conditions:Oligo GEArrays are shipped at ambient temperature. They should be stored in the enclosed disposable hybridization tube at -20 °C upon receipt.

References

1. Geimonen E, Neff S, Raymond T, Kocer SS, Gavrilovskaya IN, Mackow ER. Pathogenic and nonpathogenic hantaviruses differentially regulate endothelial cell responses. Proc Natl Acad Sci U S A. 2002 Oct 15; 99 (21): 13837-42.

2. Maragoudakis ME, Tsopanoglou NE, Andriopoulou P. Mechanism of thrombin-induced angiogenesis. Biochem Soc Trans. 2002 Apr; 30 (2): 173-177.

3. Choy JC, Granville DJ, Hunt DW, McManus BM. Endothelial cell apoptosis: biochemical characteristics and potential implications for atherosclerosis. J Mol Cell Cardiol. 2001 Sep; 33 (9): 1673-90.

4. Kim J, Wu H, Hawthorne L, Rafii S, Laurence J. Endothelial cell apoptotic genes associated with the pathogenesis of thrombotic microangiopathies: an application of oligonucleotide genechip technology. Microvasc Res. 2001 Sep; 62 (2): 83-93.

5. Biedermann BC. Vascular endothelium: checkpoint for inflammation and immunity. News Physiol Sci. 2001 Apr; 16: 84-8.

6. Malyszko J, Malyszko JS, Mysliwiec M. Markers of endothelial cell injury and thrombin activatable fibrinolysis inhibitor in nephrotic syndrome. Blood Coagul Fibrinolysis. 2002 Oct; 13 (7): 615-21.

7. Takehana K, Konishi A, Oonuki A, Noguchi M, Fujita K, Iino Y, Kobayashi T. APC0576, a novel inhibitor of NF-kappaB-dependent gene activation, prevents pro-inflammatory cytokine-induced chemokine production in human endothelial cells. Biochem Biophys Res Commun. 2002 May 10; 293 (3): 945-52.

8. Hunt BJ. The endothelium in atherogenesis. Lupus. 2000; 9 (3): 189-93.

9. Pearson JD. Normal endothelial cell function. Lupus. 2000; 9 (3): 183-8.

10. Toborek M, Kaiser S. Endothelial cell functions. Relationship to atherogenesis. Basic Res Cardiol. 1999 Oct; 94 (5): 295-314.