How It Works: RT² miRNA PCR Arrays

RT² miRNA PCR Arrays are the most reliable and sensitive gene expression profiling technology for analyzing multiple miRNA sequences simultaneously - whether you need to see the entire human miRNA genome or research application specific panels of miRNA seqeunces. The miRNA PCR Arrays can be used for research on cancer, immunology, stem cells, toxicology, biomarker discovery and validation, and phenotypic analysis of cells and transgenic animals.
See Our Complete List of miRNA PCR Arrays

The miRNA PCR array is a set of optimized real-time PCR assays, in 96-well or 384-well plates, for a miRNA panel as well as appropriate housekeeping assays and RNA quality controls. The PCR Array performs gene expression analysis with real-time RT-PCR sensitivity and the multi-gene profiling capabilities of a microarray. Simply mix your cDNA template with the appropriate ready-to-use PCR master mix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program.
Download the User Manual

What instruments do I need?
Any 96-well or 384-well real-time PCR instrument. See Instrument-Specific Setup Instructions

How It Works:

What it offers?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 88 or 376 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template

Layout and Controls: The PCR Arrays are available in both 96- and 384-well plates and are used to monitor the expression of 88 or 376 miRNA, respectively, plus four housekeeping assays for small nuclear RNA to normalize raw data. Controls are also included on each array for RNA quality and general PCR performance.

You can easily perform a PCR Array experiment in your own laboratory, or send your samples to us and take advantage of our PCR Array Gene Expression Analysis Services.

*: when using the Complete miRNA PCR Array System.