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RT² Profiler™ PCR Array System Performance
PCR Arrays stand out in their superb real-time PCR performance. In this
section, we present results representative of PCR Array performance based on the
following criteria:
- Sensitivity
- Specificity
- Reproducibility
- Amplification Efficiency
High Sensitivity:
The complete PCR Array System yields a greater-than 85 percent positive call
with as little as 25 ng as much as 5 µg of total RNA from a pathway
representing genes expressed at a low level (inflammatory cytokines and
receptors).
| Figure 1: |
PCR Arrays Let You See More Genes with
Less RNA
Different amounts of universal total RNA were characterized using the
Human Inflammatory Cytokines and Receptors PCR Array, and the percentage
of detectable genes was calculated for each RNA amount.
As little as 25 ng total RNA yields greater than an 80% positive call,
even for cytokines expressed at very low levels. |
Superb Reproducibility
The complete PCR Array System demonstrates strong correlations across technical
replicates, lots, and instruments with average correlation coefficients >
0.99 insuring reliable detection of differences in expression between biological
samples.
| |
050825_1 |
050825_2 |
050825_3 |
050825_4 |
| 060111_1 |
0.993 |
0.989 |
0.995 |
0.992 |
| 060111_2 |
0.994 |
0.990 |
0.995 |
0.992 |
| 060111_3 |
0.992 |
0.990 |
0.993 |
0.992 |
| 060111_4 |
0.993 |
0.992 |
0.994 |
0.992 |
| Figure 2: |
PCR Arrays Yield Highly Reproducible
Results
Four replicate sets of raw threshold data (1-4) obtained by two
different scientists (A & B) at two different times (050825 &
060111) on Human Drug Metabolism RT?Profiler PCR Arrays are directly
compared. The results demonstrate a high degree of correlation (R2 >
0.990). |
Distinct Specificity
The complete PCR Array System, with high quality input RNA, is guaranteed to
yield single bands of the predicted size without primer dimers or other
secondary products thus providing the most accurate real-time PCR results
possible.
| Figure 3: |
PCR Arrays Amplify A Single Gene-Specific
Product in Every Reaction.
Universal total RNA was characterized on the TGFβ / BMP Signaling
Pathway PCR Array, followed by dissociation (melt) curve analysis. PCR
Arrays specifically detect individual genes despite the expression of
related gene family members in the same RNA sample. |
Uniform PCR Amplification Efficiency
One prerequisite for PCR Array technology is the uniform PCR amplification
efficiency that allows meaningful comparison of gene expression levels of all
genes. The unique combination of SABiosciences' proprietary primer design
algorithm and rigorous testing of every primer assay by hand guarantees the high
performance of every primer assays on PCR Arrays.
| Figure 4: |
PCR Arrays Yield the Most Accurate
Results.
A representative set of assays for 4,000 genes used in RT² PCR Arrays
have an average amplification efficiency of 99% with a 95% confidence
interval from 90-110%.
Consistently high amplification efficiencies enable PCR Arrays to
accurately analyze multiple genes simultaneously utilizing the ΔΔCt
method. |
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