Prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) Detailed info
Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes PTGS2, which shows 86% - 89% amino acid sequence identity with mouse, rat, sheep, bovine, horse and rabit PTGS2 proteins, respectively. Human PTGS2 is expressed in a limited number of cell types and regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. The expression of this gene is deregulated in epithelial tumors.
Please click here to view the Band Size and Reference Positions information.
Band Size (bp): 63
Reference Position: 1502
The Reference Position is a position contained within the sequence of the amplicon relative to the start of the relevant RefSeq sequence.
The RefSeq Accession number refers to the representative sequence used to design the enclosed primers.
These primers can also generate amplicons from the following splice variants:
The RT² qPCR Primer Assay is the most reliable SYBR® Green-based quantitative real-time PCR assay for gene expression analysis. Our experimentally verified design algorithm yields gene-specific qPCR assays characterized by uniform and high PCR efficiencies and standardized amplification conditions. Every RT² Primer Assay is subjected to rigorous experimental verification.
Single product amplification of the correct size and high PCR efficiency are
guaranteed when using the appropriate RT² qPCR Master Mixes. The uniform PCR amplification efficiencies and PCR conditions of the RT² qPCR Primer Assays provide an
accurate and scalable solution for multiple gene expression analyses. Browse Primer Assays By Gene
NOTE: Do not use DEPC treated H2O.
Use high-quality, nuclease-free H2O. If you are not
sure whether your RNase, DNase-free water has been DEPC
treated, please check with the supplier.
Briefly (10-15 seconds) spin down all reagents.
For each 25-µl PCR, mix the following components in a PCR tube:
12.5
µl
RT² SYBR Green qPCR Master Mix
10.5
µl
H2O
1.0
µl
RT² First Strand cDNA (template)
1.0
µl
gene-specific RT² qPCR Primers
25.0 µl final volume
Note: If your cDNA template is not synthesized using an
SABiosciences RT² First Strand Kit, 1 to 10 µl of
cDNA (generated from up to 250 ng total RNA) may be used.
Adjust the H2O amount for a final reaction volume of 25.0 µl.
Note: For improved ease and consistency, RT² qPCR Primer
Assay-specific pre-mixes may be prepared. Scale up the
following formula for the number of reactions to be
performed within a single experiment (plus 0.1X excess
volume to allow for multiple pipetting).
Assay-Specific
Pre-Mix
12.5
µl
RT² SYBR Green qPCR Master Mix
10.5
µl
H2O
1.0
µl
gene-specific RT² qPCR Primers
24.0 µl final volume
For each 25-µl PCR reaction,
mix the following in a PCR tube
24.0
µl
Assay-Specific Pre-Mix
1.0
µl
RT² First Strand cDNA (template)
25.0 µl final volume
Quickly centrifuge and place your tubes in your real-time thermal cycler.
Enter and run the appropriate program for your real-time
instrument.
Use a two-step cycling program, for the following
instrumentation:
*Attention Roche
LightCycler 480® Users: Adjust the ramp
rate to 1ºC/sec. Please refer to
the Instrument Setup Guide at http://sabiosciences.com/pcrarrayprotocolfiles.php
for more information on other REQUIRED
changes to settings for Melt Curve
Acquisition.
Use a three-step cycling program, for
the following instrumentation:
1 The 10-minute step at 95ºC is required
to activate the HotStart DNA polymerase. 2 Detect and record SYBR Green fluorescence
from every well during the annealing step of each cycle. 3 Different instruments need different
lengths of time to detect the fluorescent signal. Choose the
appropriate time for the annealing step (55ºC) for your
instrument.
Materials
Included / Packing List
Please check the kit components immediately after you receive this
package. SABiosciences is only responsible for missing items reported within
two (2) business days of receipt.
One gene-specific PCR
primer assay (10 µM each primer, 2 primers for 200
PCR reactions) One Certificate of Performance
Storage Conditions
RT² qPCR Primer Assay are shipped at ambient temperature. For long-term storage, store RT² qPCR Primer Assays at -20 ºC.
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This product is intended for research purposes only and is not intended
for drug or diagnostic purposes or for human use. Use of kit components for
reproduction of any primer pair mix, to modify kit components for resale or
to use RT² qPCR Primer Assays to manufacture commercial products without
written approval of SABiosciences Corporation is expressly prohibited.
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Complete Array List
For each 25-µl PCR reaction,