cDNA GEArray® Focused DNA Microarray

The cDNA GEArray is our first generation pathway-focused DNA microarrays and still has the most comprehensive coverage of biological pathways and disease states for human and mouse. The nylon membrane array matrix is a permeable support with a high DNA binding capacity. The production process employs a reproducible non-contact printing technology to prevent deformations in the membrane. The carefully designed gene-specific double-stranded 250 to 600 bp cDNA fragments printed on each cDNA GEArray minimizes any cross-hybridization between spots on the same array despite the representation of closely related members of the same gene families. The optimized target synthesis and labeling protocol generates amplified and labeled cDNA only for the genes represented by the cDNA fragments on the array. The biotinylated cDNA target and the carefully designed cDNA fragments on the array correspond to the same gene-specific sequences permitting efficient and specific hybridization. The cDNA GEArray is also optimized for the popular chemiluminescent detection method, which allows the use of either X-ray film or a CCD-camera imaging system for image acquisition.

Summary of cDNA GEArray Technology Features

Feature	Specification	Benefits
Matrix	Nylon membrane	High nucleic acid binding capacity
Printing Technology	Non-contact printing	Reproducible Prevents deformations in the membrane
Probe Sets	~ 450 bp dsDNA	High specificity
Target Labeling	Biotin-cDNA from AmpoLabeling-LPR™	One-tube linear amplification Matches probe set
Detection	Chemiluminescence	Allows use of X-ray film or CCD camera

With the publication of over one hundred scientific papers employing this technology, the cDNA GEArray is proven and trusted for pathway-focused gene expression studies. However, these products are continually being updated to their Oligo GEArray equivalents. For a complete overview of the cDNA GEArray technology, click [here].

Two different spotting and density formats available:
We offer two different formats of cDNA GEArrays, the Q Series and the S Series. Only the spotting patterns differ between the two formats. The Q Series microarrays contain up to 96 genes arrayed in a unique and easily recognizable tetra-spot pattern. The S Series microarrays contain up to 480 genes arrayed as single spots. Both formats also contain similar housekeeping genes and negative controls.

The cDNA GEArrays are only available in the same format HybTube Format as the Oligo GEArray and are designed to be processed using a standard hybridization oven. The cDNA GEArrays are NOT available in the HybPlate format

How It Works:

Click here to see an overview of the experimental procedure.

Isolate total RNA from your experimental biological source material using an appropriate kit and method. Enzymatically convert the RNA into a biotinylated cDNA target with the AmpoLabeling-LPR™ Kit in a standard thermal cycler. Hybridize the labeled target to the cDNA GEArray microarrays in their own plastic disposable hybridization tube in a standard hybridization oven. Detect the microarray with the Chemiluminescent Detection Kit reagents and either a Chemi-Doc imaging system (CCD camera) or X-ray film. Acquire and analyze the data with our web-based software package.

Learn about our other GEArray technologies:
Custom GEArray [here], Oligo GEArray [here], and PCR Array [here]

• Find a pathway-focused GEArray by clicking the applications on the left or browse through our complete microarray listing.
• Customize a GEArray to suit your specific research needs. (For more information, click here.)
• Complete array processing services are also available from SABiosciences' in-house array core lab. (For more information, click here.)
• Read [this] about the reagents & equipment required to setup the GEArray in your lab.