DNA QC Mutation PCR Array

qBiomarker Somatic Mutation PCR Array: Human DNA QC Plate The Human DNA QC Plate qBiomarker Somatic Mutation PCR Array is designed to assess the quality of any DNA sample by measuring the CT values of the following 7 reference genes: BRAF, KRAS, HRAS, NRAS, MEK1, PIK3CA, and PTEN. When DNA quality is low (e.g., due to cross-linking or fragmentation), the CT values from these 7 genes will be much higher than those from the same amount of high-quality DNA. Each QC plate can analyze 12 DNA samples in the 96-well plate format (each column is for one sample) or 48 samples in the 384-well plate format. A PCR positive control assay (SMPC) is also included for each sample.

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Modify this Array    BRAF gene – Copy Number Assay KRAS gene – Copy Number Assay HRAS gene – Copy Number Assay NRAS gene – Copy Number Assay MEK1 gene – Copy Number Assay PIK3CA gene – Copy Number Assay PTEN gene – Copy Number Assay

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Overview of the qBiomarker Somatic Mutation PCR Array / Assay Protocol Overview of the qBiomarker Somatic Mutation PCR Array / Assay Protocol. The procedure involves DNA extraction (QIAGEN QIAamp DNA Mini Kit or FFPE Tissue Kit is recommended), an optional amplification (QIAGEN REPLI-g kit or REPLI-g UltraFast kit is recommended) step for DNA isolated from fresh samples, qPCR detection on qBiomarker Somatic Mutation PCR Arrays or Assays, and data analysis (using the qBiomarker Somatic Mutation Data Analysis Template). An optional DNA sample QC step immediately before the detection array or assay setup allows the user to qualify the DNA samples. Principle of Mutant Discrimination with ARMS® Back to Top

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The PKB/AKT pathway in cancer.Carnero A. Curr Pharm Des. 2010 Jan; 16(1):34-44 BRAF, a target in melanoma: implications for solid tumor drug development. Flaherty KT, McArthur G. Cancer. 2010 Jul 13. [Epub ahead of print] Clinical relevance of KRAS in human cancers. Jancík S, Drábek J, Radzioch D, Hajdúch M. J Biomed Biotechnol. 2010; 2010:150960. Oncogenic Ras in tumour progression and metastasis. Giehl K. Biol Chem. 2005 Mar; 386(3):193-205 MEK1 mutations confer resistance to MEK and B-RAF inhibition. Emery CM, Vijayendran KG, Zipser MC, Sawyer AM, Niu L, Kim JJ, Hatton C, Chopra R, Oberholzer PA, Karpova MB, MacConaill LE, Zhang J, Gray NS, Sellers WR, Dummer R, Garraway LA. Proc Natl Acad Sci U S A. 2009 Dec 1; 106(48):20411-6 PIK3CA mutations in human solid tumors: role in sensitivity to various therapeutic approaches. Ligresti G, Militello L, Steelman LS, Cavallaro A, Basile F, Nicoletti F, Stivala F, McCubrey JA, Libra M. Cell Cycle. 2009 May 1; 8(9):1352-8 Oncogenic mutations as predictive factors in colorectal cancer. Lièvre A, Blons H, Laurent-Puig P. Oncogene. 2010 May 27; 29(21):3033-43  PI(3)King Apart PTEN's Role in Cancer. Zhang S, Yu D. Clin Cancer Res. 2010 Jul 8. [Epub ahead of print] Back to Top

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User Manual qBiomarker Somatic Mutation PCR Array System (PDF) Data Analysis qBiomarker Somatic Mutation PCR Array Data Analysis Software Application Data Detection Limits, Cancer Pathways FAQ Frequently Asked Questions about Somatic Mutation Assays and Arrays Webinar qBiomarker Somatic Mutation Analysis: Real-World Application Data Slide Presentation> Presentation about qBiomarker Somatic Mutation Assays and Arrays (PDF) Scientific Poster A Novel Tool for Pathway-Focused Cancer Mutation Profiling (PDF) Presented at American Association for Cancer Research 2011 White Paper Rapid and accurate cancer somatic mutation profiling with the qBiomarker Somatic Mutation PCR Array (PDF) Product Profile For screening biology-focused panels of gene mutations (PDF) Back to Top