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Cignal 45-Pathway Reporter Array

 
Cignal 45-Pathway Reporter Array: CCA-901L

The Signal Transduction Reporter Array allows for the comprehensive analysis of 45 signaling pathways. By screening pathway activities simultaneously, scientists quickly identify relevant pathways for further analysis. Therefore, Cignal Finder Arrays pinpoint the pathways that are perturbed by a specific gene or drug. The plate format facilitates shorter transfection protocols and high-throughput analysis.

See the List of Pathways / Well Positions
 

Kit Components How It Works Manual & Resources Related products
 
Component  Specification  Concentration and total volume
Each of the 45 Reporter Assays A mixture of an inducible transcription factor responsive firefly luciferase reporter and constitutively expressing Renilla construct (20:1).
200 ng
Negative control A mixture of non-inducible firefly luciferase reporter and constitutively expressing Renilla construct (20:1). 200 ng
Positive control A mixture of a constitutively expressing GFP construct, constitutively expressing firefly luciferase construct, and constitutively expressing Renilla luciferase construct (20:1:1). 200 ng

The Cignal 45-Pathway Reporter Array is available in either two (2) or twelve (12) plate packs.

 

Kit Components How It Works Manual & Resources Related Products
 

Cignal Finder Array - Plate Format
The plate format of the Cignal Finder Arrays are delivered in a 96-well cell culture plate. Each reporter and control assay is dried down in two wells of the plate.

Simple Procedure:

  • Transfect Cignal Reporter Assays and test nucleic acids into cells
  • Treat with protein, peptide, or small molecule of interest
  • Measure pathway activity using luciferase enzyme activity assays

All reporter assays are based on dual-luciferase technology. Each reporter consists of a mixture of a pathway-focused transcription factor-responsive firefly luciferase construct and a constitutively expressing Renilla luciferase construct.

Data Analysis:

SABiosciences has developed a complimentary excel spreadsheet to assist in the analysis of the 45 pathways.

Down load the Data Analysis Software

Dual-luciferase results are calculated for each transfectant. The change in the activity of each signaling pathway is determined by comparing the normalized luciferase activities of the reporter in treated versus untreated transfectants.

The identically treated negative control serves as a specificity control. The positive control serves as a control for transfection efficiency, by monitoring GFP expression, as well as a positive control for both the firefly and Renilla luciferase assays.


Cignal Array Example Experiments:

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Kit Components How It Works Manual & Resources Related Products
 
User Manuals Cignal Finder™ 10-Pathway Reporter Arrays (PDF)
White Paper Cignal Reporter Assay Kit: A High Performance Tool for Assessing the Functions of Genes, Biologics and Small Molecule Compounds (PDF)
Web Seminar Attend a live on-line seminar hosted by an Application Scientist about Cignal Reporters
Technical Brochure Cignal Reporter Assay Kits Technical Brochure (PDF)
Power Point Cignal™ Cell-Based Assays for Rapidly Analyzing Signaling Pathway Activity (PDF)
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Kit Components How It Works Manual & Resources Related Products
 
Pathway reporters Complete list of signaling pathway reporters
Attractene Transfection Achieve high transfection efficiencies and minimal cytotoxicity in your transfections
10-Pathway Arrays Simultaneously measure the activities of ten (10) signaling pathways in a single experiment
shRNA Plasmids Pathway related gene knockdowns - Guaranteed 70% gene knockdown

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