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Cignal Finder Array - Plate Format
The plate format of the Cignal Finder Arrays are delivered in a 96-well cell
culture plate. Each reporter and control assay is dried down in two wells of
the plate.
Simple Procedure:
- Transfect Cignal Reporter Assays and test nucleic acids into cells
- Treat with protein, peptide, or small molecule of interest
- Measure pathway activity using luciferase enzyme activity assays
All reporter assays are based on dual-luciferase technology. Each
reporter consists of a mixture of a pathway-focused transcription
factor-responsive firefly luciferase construct and a constitutively
expressing Renilla luciferase construct.
Data Analysis:
SABiosciences has developed a complimentary excel spreadsheet to assist
in the analysis of the 45 pathways.
Down
load the Data Analysis Software
Dual-luciferase results are calculated for each transfectant. The change
in the activity of each signaling pathway is determined by comparing the
normalized luciferase activities of the reporter in treated versus untreated
transfectants.
The identically treated negative control serves as a specificity control.
The positive control serves as a control for transfection efficiency, by
monitoring GFP expression, as well as a positive control for both the
firefly and Renilla luciferase assays.
Cignal Array Example Experiments:
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Complete Array List
