Human Apoptosis PCR Array, TaqMan® Version The RT² Profiler PCR Array Human Apoptosis, TaqMan® Version profiles the expression of 84 key genes involved in apoptosis, or programmed cell death. The array includes the TNF ligands and their receptors; members of the bcl-2, caspase, IAP, TRAF, CARD, death domain, death effector domain, and CIDE families; as well as genes involved in the p53 and ATM pathways. The TaqMan PCR Arrays are optimized to deliver the same high level of performance on any Bio-Rad, Stratagene, Roche, and Eppendorf instrumentation, as well as ABI instrumentation. Using real-time PCR, you can easily and reliably analyze expression of a focused panel of genes related to apoptosis with this array. Both 96-well and 384-well ( 4 X 96 ) formats are available. See the complete PCR Array list User Manual

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Gene Table TNF Ligand Family: CD40LG (TNFSF5), FASLG (TNFSF6), LTA, TNFSF10, CD70, TNFSF8. TNF Receptor Family: CD40 (TNFRSF5), FAS (TNFRSF6), LTBR, TNFRSF10A, TNFRSF10B, TNFRSF11B, TNFRSF1A, TNFRSF1B, TNFRSF21, TNFRSF25, CD27, TNFRSF9. Bcl-2 Family: BAD, BAG1, BAG3, BAG4, BAK1, BAX, BCL2, BCL2A1, BCL2L1, BCL2L10, BCL2L11, BCL2L2, BCLAF1, BID, BIK, BNIP1, BNIP2, BNIP3, BNIP3L, HRK, MCL1. Caspase Family: CASP1, CASP10, CASP14, CASP2, CASP3, CASP4, CASP5, CASP6, CASP7, CASP8, CASP9. IAP Family: BIRC2, BIRC3, BIRC4, BIRC6, BIRC8, DIABLO, NAIP. CARD Family: APAF1, BCL10, BIRC2, BIRC3, NOD1, CARD6, CARD8, CASP1, CASP2, CASP4, CASP5, CASP9, CRADD, NOL3, PYCARD, RIPK2. Death Domain Family: CRADD, DAPK1, FADD, FAS (TNFRSF6), TNFRSF10A, TNFRSF10B, TNFRSF11B, TNFRSF1A, TNFRSF21, TNFRSF25, TRADD. Death Effector Domain Family: CASP8, CASP10, CFLAR, FADD. CIDE Domain Family: CIDEA, CIDEB, DFFA. p53 and DNA Damage Response: ABL1, AKT1, APAF1, BAD, BAX, BCL2, BCL2L1, BID, CASP3, CASP6, CASP7, CASP9, GADD45A, TP53, TP53BP2, TP73. Anti-Apoptosis: AKT1, BAG1, BAG3, BCL2, BCL2A1, BCL2L1, BCL2L10, BCL2L2, BFAR, NAIP, BIRC2, BIRC3, BIRC4, BIRC6, BIRC8, BNIP1, BNIP2, BNIP3, BRAF, CASP2, CFLAR, FAS (TNFRSF6), IGF1R, MCL1, CD27, DIABLO.   Gene Table

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"If you have access to ANY real-time PCR instrument, you can use PCR Arrays to perform apoptotic gene expression profiling with TaqMan-based detection." PCR Arrays are the most reliable and sensitive gene expression profiling technology for analyzing a panel of genes in signal transduction pathways, biological process or disease related gene networks. The PCR Arrays can be used for research on cancer, immunology, stem cells, toxicology, biomarker discovery and validation, and phenotypic analysis of cells and transgenic animals. You can use any 96-well or 384-well real-time PCR instrument. Find the Setup Instructions and Protocol Files for your real-time PCR instrument How it Works Performance Data How it Works The TaqMan version of the PCR Array is a set of optimized real-time TaqMan PCR assays, each designed by SABiosciences' superior bioinformatic algorithms and synthesized by a licensed provider. The assays are arranged on 96-well or 384-well plates for pathway or disease focused genes as well as appropriate RNA quality controls. The PCR array performs gene expression analysis with real-time PCR sensitivity and the multi-gene profiling capability of a microarray. Simply mix your cDNA template with the appropriate ready-to-use PCR master mix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program. (Download user manual) Figure 1: How PCR Arrays Work - Protocol Chart Performance Data The TaqMan PCR Array combines: Stringent SABiosciences primer & probe design and pathway bioinformatics Trusted TaqMan probe synthesis from a licensed provider, Applied Biosystems We design minor-groove binding probes with optimal probe length, melting temperature, GC content and other important sequence-related parameters. Then, we design amplification primers around the probe sequence using our rigorous primer design algorithm originally fine-tuned for the exquisite specificity required by SYBR Green detection. Quality control assays, first with primers alone and then with primers and probe, insure consistently high amplification efficiencies for reliable results. Combining our rigorous sequence design and quality control with licensed probe production gives you confidence in the quality of the TaqMan-based assays in SABiosciences' PCR Arrays. The TaqMan version of the PCR Arrays provide the same level of real-time PCR performance and the same experimental results as the TaqMan Express Plates from Applied Biosystems and the SYBR Green-based RT² Profiler PCR Arrays from SABiosciences. Amplification Efficiency   Figure 2: All Assays in the TaqMan version of the PCR Array Demonstrate Consistently High Amplification Efficiencies for Reliable Results. The 384-well format of the Human Apoptosis PCR Array, TaqMan® Version was used to characterize four different amounts of genomic DNA on either the ABI 7900HT or the Roche LightCycler 480. Amplification efficiencies were calculated from the slope of the standard or calibration curve. The results from a representative set of assays on the array are displayed and demonstrate that all values lie with 10 percent of the average amplification efficiency of 97.4 percent. Same Results from SYBR Green and TaqMan Versions of the PCR Array A B The SYBR Green and TaqMan version of the PCR Arrays Yield the Same Fold-Change Results. Fold-difference results were obtained on either the ABI 7500 (A) or the Roche LightCycler 480 (B) real-time PCR instruments using the two MAQC reference RNA samples analyzed on both Human Apoptosis PCR Arrays. The fold-difference (or fold-change or FC) results between the two RNA samples were calculated using the Ct method from the average ΔΔCt value for each assay on the arrays. The results from the two detection technologies were plotted against one another and fit to a straight line with a slope of one. The comparisons of the fold-change results between SYBR Green and TaqMan detection methods have correlation factors greater than 0.9 indicating a high level of agreement. Why use SABiosciences' PCR Arrays instead of ABI TaqMan Express Plates? Instrument Compatibility      Use TaqMan-based real-time PCR Arrays even if you don't have an ABI instrument Superb Data Analysis Software      We won't leave you out on your own to get results from the array data Built-In RNA Quality Control Assays      Assess genomic DNA contamination, RT efficiency, & PCR conditions in every run Same Results at Lower Price      Biological answers & real-time PCR performance are detection technology-independent Should I use a SYBR Green or TaqMan versions of PCR Array? Take Your Pick - Same Results & Performance Both SYBR Green and TaqMan version of PCR Arrays perform the same, yield the same biological results, and support any real-time instrument. So, take your pick based on whatever you've read or seen. PCR Arrays support your experiments in apoptosis research with either detection method. Stop Waiting for Pre- or Self-Optimized Assays Researchers currently using SYBR Green already know how PCR Arrays can help them. If you study apoptosis and currently use TaqMan, you don't have to analyze one gene at a time anymore or wait weeks for a licensed provider to design, test, and ship multiple assays. PCR Arrays let you profile 84 apoptotic genes in one sample all at the same time in one instrument run. The pre-printed cataloged PCR Arrays are available off-the-shelf and ship the next business day. SYBR Green for Price & Specificity; TaqMan for Throughput Still can't decide which PCR Array to use? SYBR Green offers a lower price and the ability to double check the specificity of every assay in the array with melt curves, while TaqMan offers a shorter cycling program for slightly greater throughput. Back to Top

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User Manual RT² Profiler PCR Array, TaqMan® Version (PDF) Data Analysis Free PCR Array Data Analysis Software (Web & Excel based) Instrument Setup Instructions Materials & Equipment Required for our PCR products PCR Array Instrument Setup Instructions & Protocol Files FAQ FAQ about Real-Time PCR and Troubleshooting for our PCR products Web Seminars Attend a live on-line seminar hosted by an Applications Scientist about PCR Powerpoint Presentations PCR Technology Overview Presentations Pathway Central Pathway reviews and presentation-ready pathway maps Back to Top

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The complete PCR Array System insures that you will get good results from your pathway-focused and TaqMan-based gene expression analysis experiment the first time, and every time guaranteed. The Complete TaqMan version of the PCR Array System Includes: Human Apoptosis PCR Array, TaqMan® Version RT² First Strand Kit Sensitive qRT-PCR with RNA Quality Control and NO Genomic DNA Worries RT² qPCR Master Mixes, TaqMan® Optimized Instrument-specific PCR master mixes insure high-efficiency, gene-specific amplification for the most accurate real-time PCR results. PCR Array Data Analysis Software (Excel & Web based) Our convenient and high-quality real-time RT-PCR accessory products complete the PCR Array System. The other components needed for real-time PCR analysis are RT² qPCR master mix and the first strand synthesis kit (C-03). Other recommended accessory products RT² qPCR-Grade RNA Isolation Kit From the experts in isolating RNA free of genomic DNA for real-time RT-PCR Individual Assays Validated and Certified RT² SYBR Green Assays for the genes on this array SureSilencing shRNA Plasmids Pathway related gene knockout - guaranteed > 70% knockdown Back to Top