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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

August 2016
Monday
Tuesday
Wednesday
Thursday
Friday

1

PAXgene Tissue

Nucleic acid isolation from PCR inhibitor-rich sample types

2

3

NGS: an introduction to technology and applications

4

5

8

gDNA isolation from solid tissue samples

Microbiome: From identification to characterization

9

CTC Technology overview

10

RNAseq targeted panels

11

Technologies for epigenetic research

cfDNA handling and purification

12

15

Profiling microbial species in hospital acquired infections

16

Standardize sample quality

Practical hints for successful PCR

17

Basics of sample prep in RNA research

Analyzing fusion genes with NGS

18

DNA Methylation analysis

QIAsymphony

19

22

Multiplex PCR as a tool for genotyping

cfDNA analysis and interpretation

23

Controls and novel solutions for real-time qPCR

Innate immune system

24

QC for RNA sample

Single cell technology introduction

25

PyroMark in genotyping

Analysis of circulating cell-free DNA with NGS

26

29

QPCR Introduction

30

Real-time qPCR data analysis and interpretation

T cell and B cell

31

RNA integrity and quality

Current Seminar Titles Available:

Focus Title
1.  QPCR IntroductionIntroduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)
2.  RNA isolationRNA Integrity and Quality – Standardize RNA Quality Control
3.  Onestep ahead RT-PCROne step ahead for your RT-PCR
4.  RNAseq technologyDigital RNASeq: Technology introduction
5.  RNAseq technologyDigital RNAseq for gene expression profiling: Workflow and applications
6.  RNAseq technologyMolecular insight into gene expression using Digital RNAseq: Data analysis tutorial
7.  QuantiNova PCR kit The importance of controls and novel solutions for successful real-time qPCR
8.  Liquid BiopsiesNew Technology and workflow for integrated collection, stabilization, and purification of circulating cell-free DNA
9.  Liquid BiopsiesAnalysis and interpretation of cell free DNA
10.  Liquid BiopsiesLiquid biopsy: overcome challenges of circulating DNA with automated and standardized extraction processes
11.  Next-generation sequencingNew and highly integrated tools for sensitive next-generation sequencing: Analysis of circulating cell-free DNA
12.  Single cell analysisSingle cell technology introduction
13.  Microbial DNA IsolationNucleic acid isolation from PCR inhibitor-rich sample types
14.  QIAseq RNAScanAnalyzing fusion genes with next-generation sequencing technology
15.  Sample Quality ControlKeep control over the quality and reproducibility of your research by standardizing your nucleic acid samples
16.  Sample Quality ControlRNA Quality Control for results you can rely on - Interpret your gene-expression results with confidence
17.  Sample Quality ControlMaximize the success of your NGS experiments with state-of-the-art NGS library quality control solutions
18.  Sample Quality ControlNucleic acids quantification from FFPE samples; are you doing it right?
19.  QIAseq Targeted DNA PanelsDigital Next-Generation Sequencing for targeted enrichment, an Introduction to Technology
20.  QIAseq Targeted DNA PanelsDigital DNA-seq Technology - Targeted Enrichment for Cancer Research
21.  Sample PrepCancer Research and the Challenges of FFPE Samples
22.  QIAseq Library PrepIntroduction to Next Generation Sequencing (NGS) technology
23.  QIAseq Library PrepInnovative NGS Library Construction Technology
24.  QIAseq Library PrepAdvanced NGS library prep for challenging samples
25.  miScript miRNA ArraysIntroduction to microRNA
26.  Microbial DNA PCR ArraysStudying the microbiome: tools for microbial detection and host-response analysis
27.  Single cell analysisHow to analyze heterogeneous tumor samples?
28.  AdnaTestsCTC Detection and molecular characterisation – Challenges and Solutions
29.  QIAGEN ServiceRNA-seq for biomarker discovery
30.  Stool sample prepMinimize biases in comparing metagenomics with metatranscriptomics: parallel isolation of DNA and RNA from stool sample

Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)

Real-time polymerase chain reaction (qPCR) is the most sensitive and reliable method for the detection and quantification of nucleic acids. This 45-minute webinar introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that will be covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications, and factors for success. This webinar is for both beginners that are new to real-time PCR as well as experienced users.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, September 14, 2016 at 1:00 PM Eastern    Status: Available Reserve

RNA Integrity and Quality – Standardize RNA Quality Control

RNA integrity and quality are critical to obtain meaningful and reliable downstream data. This webinar discusses the challenges and considerations of handling RNA samples, the need for quality control analysis and common methods for RNA integrity and quality assessment. The QIAxcel Advanced System will be introduced to automate the process of RNA sample integrity analysis and obtain objective quality measurement. Application data will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, August 31, 2016 at 9:30 AM Eastern    Status: Available Reserve

One step ahead for your RT-PCR

"At the heart of every successful discovery lie the seeds of innovation. At QIAGEN, we are constantly developing new methods that allow researchers to gain forward momentum with their research. Whether you’re studying gene expression or performing viral RNA analysis, the success of your experiment depends on the ability to analyze your sample with the highest standards of sensitivity and specificity so that you can have confidence in your data. To help you generate valuable insights from gene expression profiling and viral RNA analysis, we introduce the brand new QIAGEN OneStep Ahead RT-PCR Kit – the first hot-start reverse transcriptase kit on the market. Continuing the success story of its first- generation predecessor (QIAGEN OneStep RT-PCR Kit), the QIAGEN OneStep Ahead RT-PCR Kit is equipped with compelling new features that afford maximum convenience and ease of use, while delivering unmatched sensitivity and specificity. With a total reaction time of 1 hour, higher sequence accuracy and the ability to amplify amplicons of up to 4 kb without tedious optimization, you can get one step closer to publishing your findings with this new solution. For increased convenience, the kit comes in an all-in-one tube format along with a built-in pipetting control. Stay one step ahead of your peers and make significant advances in your research with the QIAGEN OneStep Ahead RT-PCR Kit! In this webinar we will introduce the new kit in detail and discuss its features and benefits. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 26, 2016 at 9:30 AM Eastern    Status: Available Reserve
Tuesday, October 25, 2016 at 1:00 PM Eastern    Status: Available Reserve

Digital RNASeq: Technology introduction

QIAseq RNA is a revolutionary turnkey solution for digital gene expression analysis by NGS. From 10 genes to 1000, from one sample to 100, QIAseq RNA delivers precise results on both ION and Illumina sequencing platforms. The data from QIAseq RNA is directly comparable to expression analysis derived from whole transcriptome sequencing or by qRTPCR, only better, cheaper, faster and more flexible. This webinar will describe the principles of digital expression analysis by NGS, and review the features and benefits of the QIAseq system, options available and the integrated data analysis package.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, October 6, 2016 at 9:30 AM Eastern    Status: Available Reserve

Digital RNAseq for gene expression profiling: Workflow and applications

Traditional RNA sequencing (RNA-Seq) is a powerful tool for expression profiling, but is hindered by PCR amplification bias and inaccuracy at low expressing genes. QIAseq RNA is a flexible and precise tool developed for mitigating these complications, allowing digital gene expression analysis. This in-depth webinar will cover sample requirements, experimental design, NGS platform-specific challenges and workflow for gene enrichment, library prep and sequencing. The applications of QIASeq RNA Panels in cancer research, stem cell differentiation and elucidating the effects small molecules on signaling pathways will be highlighted.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, October 13, 2016 at 9:30 AM Eastern    Status: Available Reserve

Molecular insight into gene expression using Digital RNAseq: Data analysis tutorial

Gene expression profiling is the key to understanding biological pathways and complex cellular systems. In this webinar, we will discuss the challenges of targeted RNA-seq data analysis and present the solutions provided by the QIAGEN automated online data analysis tools. Using raw sequencing data from targeted sequencing, the output of the QIAseq primary data analysis tool and the options in QIAseq secondary analysis, such as normalization strategies, will be described. The use of Ingenuity Pathway Analysis (IPA) to unlock the molecular insights buried in experimental data by quickly identifying relationships, mechanisms, functions, and pathways of relevance will be shown with an example.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, October 27, 2016 at 9:30 AM Eastern    Status: Available Reserve

The importance of controls and novel solutions for successful real-time qPCR

"The increasing demand for streamlined, monitored, and ultrafast qPCR procedures requires high-performance, real-time quantitative RT and PCR chemistries. Particularly procedures utilizing generic kits for gene expression analysis should include in-process safety measures to avoid variables and control accuracy of procedures and results. This webinar presents innovative solutions for one-step and two-step RT-PCR that significantly enhance performance and reliability in qRT-PCR. The new QuantiNova kit family offers a combination of various integrated safety features to remove variables and prevent artifacts. Internal Control RNA, removal of genomic DNA, room temperature set up capability for RT-PCR, and a built-in visual pipetting control verify accurate procedures, ensuring reliable gene expression profiling. This webinar explains the principles of the technologies and shows data demonstrating performance in qRT-PCR. Find out how you can verify accurate performance in qRT-PCR and improve your results."

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 6, 2016 at 9:30 AM Eastern    Status: Available Reserve
Tuesday, October 4, 2016 at 1:00 PM Eastern    Status: Available Reserve

New Technology and workflow for integrated collection, stabilization, and purification of circulating cell-free DNA

Research into non-invasive prenatal testing (NIPT) and circulating tumor DNA (ctDNA) testing based on circulating cell-free DNA (ccfDNA) is rapidly expanding. However, detection and quantification of ccfDNA is compromised by the release of genomic DNA (gDNA) from lymphocytes due to mechanical lysis or apoptosis during blood collection, storage and transport. PreAnalytiX has developed the PAXgene® Blood ccfDNA System, consisting of the PAXgene Blood ccfDNA Tube, a plastic blood collection tube with a unique, non-crosslinking chemistry that preserves extracellular levels of ccfDNA and prevents the release of intracellular DNA from cells into the plasma, and the QIAsymphony® PAXgene Blood ccfDNA Kit for automated ccfDNA extraction from up to 5 ml of plasma. In this webinar, this new technology development will be presented in comparison to other existing technologies.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 8, 2016 at 9:30 AM Eastern    Status: Available Reserve
Thursday, October 6, 2016 at 1:00 PM Eastern    Status: Available Reserve

Analysis and interpretation of cell free DNA

Identification and monitoring of cancer mutations from cell free DNA-Seq data is a key application in liquid biopsy. In this part of the webinar we will show how mutations can be best identified from this type of data and how they can be interpreted. Furthermore, potential challenges when analyzing this type of data will be discussed together with relevant strategies.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 29, 2016 at 9:30 AM Eastern    Status: Available Reserve
Thursday, October 27, 2016 at 1:00 PM Eastern    Status: Available Reserve

Liquid biopsy: overcome challenges of circulating DNA with automated and standardized extraction processes

"Circulating, cell-free DNA (cfDNA) originating from malignant tumors, a developing fetus and also from inflammatory tissues, is present in the cell-free nucleic acids in plasma, serum and other body fluids and is considered a “liquid biopsy”. Access to cfDNA for analysis allows for specific detection of certain disease states based on a simple blood sample. Circulating cell-free DNA shows distinctive properties – it is present mostly as shorter fragments of less than 500 bp and the concentration of cfDNA in a plasma or serum sample is low (approximately 1–100 ng/ml) compared to cellular materials and varies considerably between different individuals. Because of their fragmented nature and low concentration, cfDNA presents a particular challenge for efficient extraction / purification and quantification, such as by qPCR. We present data on solutions for the following critical problems concerning the purification of cfDNA for research and molecular diagnostic applications: • Pre-analytical workflow (blood processing) for analyzing cfDNA • Optimization of cfDNA extraction from plasma samples: low target concentrations require efficient cfDNA enrichment from larger sample volumes • Novel automated extraction of cfDNA using the QIAsymphony SP instrument for liquid biopsy diagnostic applications "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 15, 2016 at 9:30 AM Eastern    Status: Available Reserve
Thursday, October 13, 2016 at 1:00 PM Eastern    Status: Available Reserve

New and highly integrated tools for sensitive next-generation sequencing: Analysis of circulating cell-free DNA

"Next-generation sequencing of free circulating DNA from plasma, serum and urine has been quickly adopted for cancer testing as well as for noninvasive prenatal testing (NIPT). For cancer patients, this method enables noninvasive diagnoses of actionable mutants to personalize anti-tumor therapies. In addition, this approach could be used to monitor molecular changes during cancer treatment or tumor progression. The concentration of free circulating DNA in liquid biopsies is usually very low. This highlights the critical need to use optimized protocols for cfDNA extraction to construct NGS DNA libraries. In this webinar, we will introduce new technical solutions for Ion Torrent as well as Illumina sequencing platforms that combine optimized cfDNA isolation, highly advanced library construction, unbiased library amplification and integrated data analysis to reliably analyze cfDNA samples with high sensitivity. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 22, 2016 at 9:30 AM Eastern    Status: Available Reserve
Wednesday, October 5, 2016 at 11:00 AM Eastern    Status: Available Reserve
Thursday, October 20, 2016 at 1:00 PM Eastern    Status: Available Reserve

Single cell technology introduction

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 15, 2016 at 1:00 PM Eastern    Status: Available Reserve

Nucleic acid isolation from PCR inhibitor-rich sample types

An accurate molecular analysis of microbial constituents within a particular community is contingent upon nucleic acid extraction methodologies that are non-biased and have high-yield. Only by ensuring that all species and classes of microorganisms present in a sample are effectively lysed during extraction will one be able to reliably assess the composition of that sample. An additional challenge faced in nucleic acid extraction is the presence of persistent, co-purifying polymerase inhibitors endogenous to one’s sample. This presentation will focus on nucleic acid extraction tools developed by MO BIO Laboratories that facilitate accurate non-biased community analysis and eliminate common amplification problems via the depletion of endogenous polymerase inhibitors using our patented Inhibitor Removal Technology.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, September 21, 2016 at 1:00 PM Eastern    Status: Available Reserve

Analyzing fusion genes with next-generation sequencing technology

"Fusion genes are hybrid genes formed by the fusion of two separate genes. Translocation, interstitial deletion and chromosomal inversions are some of the genetic events that can lead to the formation of fusion genes. The occurrence of fusion genes and its implications in cancer have already been known, but the emergence of NGS technology – especially RNA sequencing – offers the potential to detect novel gene fusions. You can learn more about fusion genes and applying NGS to detect them at our upcoming webinar, presented by Raed Samara, Ph.D., QIAGEN’s Global Product Manager for NGS technologies. This webinar will cover: 1. Fusion genes: what they are and a historical perspective 2. Fusion gene detection: the current status 3. RNA sequencing vs. digital RNA sequencing 4. How to detect and accurately quantify novel fusion genes in your sample ? "

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 6, 2016 at 1:00 PM Eastern    Status: Available Reserve
Tuesday, October 4, 2016 at 11:00 AM Eastern    Status: Available Reserve
Thursday, October 20, 2016 at 9:30 AM Eastern    Status: Available Reserve

Keep control over the quality and reproducibility of your research by standardizing your nucleic acid samples

"From starting material to final results, every analysis workflow is a journey to unlock the biological information within your samples without altering it, and quality results are only achieved from quality samples. Within each step lie challenges directly related to the sample type and analysis technologies, and at each step, there is potential for many things to go wrong, jeopardizing your experiments, results and reputation. Therefore, standardizing samples and performing relevant quality control after critical steps is of utmost importance to ensure quality and reproducibility of results as well as reliable interpretation. In this webinar, we will introduce you to the main sample quality parameters, their respective impact on downstream applications, how to monitor them and what are the advantages automating quality control along complex workflows."

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, September 7, 2016 at 9:30 AM Eastern    Status: Available Reserve
Wednesday, October 5, 2016 at 1:00 PM Eastern    Status: Available Reserve

RNA Quality Control for results you can rely on - Interpret your gene-expression results with confidence

"By their very nature RNA molecules, especially mRNA and regulator RNA, are labile and can be highly unstable and sensitive to heat, UV and RNase contamination. The quality, relevance and scientific impact of gene-expression results directly depends on one’s ability to extract RNA without losing any fraction of interest while preserving the integrity of the biological information it carries. RNA quality control is thus critical to ensure quality results and turning these results into actionable insights with confidence. In this webinar, we will introduce you to the main parameters influencing RNA-based assays, their respective impact on downstream applications, how to monitor them and the advantages of automation for quality control along complex workflows."

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, September 14, 2016 at 9:30 AM Eastern    Status: Available Reserve
Wednesday, October 12, 2016 at 1:00 PM Eastern    Status: Available Reserve

Maximize the success of your NGS experiments with state-of-the-art NGS library quality control solutions

"Next generation sequencing workflows are long and complex, multistep procedures to prepare NGS libraries of specific quality in term of molarity, purity and size distribution. The quality of the NGS library is the factor with the most influence on the success of the sequencing run, affecting both the sequence validity and the number of reads. Quality control procedures tracking success of library preparation steps ensure that only samples of good quality are processed into the resources-intensive sequencing runs, because discovering a problem with your libraries when interpreting data is synonym to significant waste of time, resources and of your precious template. In this webinar, we will introduce QC procedures for automated, objective and reliable NGS library quality control in order to bring your NGS QC to a new level of convenience and throughput. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, September 21, 2016 at 9:30 AM Eastern    Status: Available Reserve
Wednesday, October 19, 2016 at 1:00 PM Eastern    Status: Available Reserve

Nucleic acids quantification from FFPE samples; are you doing it right?

"Formalin-fixation and paraffin-embedding is a standard method for long-term preservation of tissue biopsies and these stored samples are a valuable tool for studying diseases such as cancer, especially when they are histologically and pathologically well characterized, and follow-up clinical data is available. The quality of nucleic acids extracted from FFPE samples is influenced by a number of factors, including how the samples were handled before, during and after fixation & embedding. Moreover, there are several difficulties when purifying nucleic acids from FFPE samples as the chemicals & temperature used during the process can degrade the DNA. In this webinar we will discuss the variability in quantity and purity of DNA purified from FFPE material. We will show data from different quantification and quality control methods and demonstrate the impact of inaccurate quantification on downstream results and how to overcome these challenges. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, September 28, 2016 at 9:30 AM Eastern    Status: Available Reserve
Wednesday, October 26, 2016 at 1:00 PM Eastern    Status: Available Reserve

Digital Next-Generation Sequencing for targeted enrichment, an Introduction to Technology

Next-generation sequencing (NGS) is being utilized for numerous new and exciting applications. Targeted DNA sequencing with panels has particularly emerged as a powerful approach to detect low-frequency variants. This webinar provides a technical overview of DNA preprocessing, template preparation, sequencing, and data analysis. It will cover the applications of NGS technologies, including guidelines for how to select the technology that will best address your biological question, with a focus on a novel digital sequencing approach -targeted enrichment based on a random molecular barcode technology.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 1, 2016 at 1:00 PM Eastern    Status: Available Reserve

Digital DNA-seq Technology - Targeted Enrichment for Cancer Research

Targeted DNA sequencing has become a powerful approach by achieving high coverage of the region of interest while keeping the cost of sequencing and complexity of data interpretation manageable. However, existing PCR-based target enrichment approaches introduce errors due to PCR amplification bias and artifacts, which significantly affects quantification accuracy and limit the ability to confidently detect low-frequency DNA variants. This webinar introduces a new digital sequencing approach that is based on the use of unique molecular indices (UMIs) - QIAseq Targeted DNA Panels. With UMIs, each unique DNA molecule is barcoded before any amplification takes place to correct for PCR errors. Detailed workflow and applications in cancer research will be presented. Join us and learn about this exciting novel digital DNAseq technology.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 8, 2016 at 1:00 PM Eastern    Status: Available Reserve
Monday, October 3, 2016 at 11:00 AM Eastern    Status: Available Reserve

Cancer Research and the Challenges of FFPE Samples

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 12, 2016 at 9:30 AM Eastern    Status: Available Reserve
Tuesday, October 11, 2016 at 1:00 PM Eastern    Status: Available Reserve

Introduction to Next Generation Sequencing (NGS) technology

"Next-generation sequencing (NGS) technology allows the rapid analysis of billions of DNA sequences in a high-throughput approach with high accuracy to generate as much as terabases of sequence information in only one NGS sequencing run. Such information can not only be used in basic and biomedical research fields, but also in clinics to finally benefit human health in a profound way. The continuous evolution of NGS technology has led to an enormous diversification in NGS applications and dramatically decreased the costs to sequence a complete human genome. In this presentation, we will discuss the following major topics: • Basic overview of NGS sequencing technologies • Next-generation sequencing workflow • Spectrum of NGS applications • QIAGEN universal NGS solutions"

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 13, 2016 at 9:30 AM Eastern    Status: Available Reserve
Monday, October 17, 2016 at 1:00 PM Eastern    Status: Available Reserve

Innovative NGS Library Construction Technology

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 20, 2016 at 9:30 AM Eastern    Status: Available Reserve
Monday, October 24, 2016 at 1:00 PM Eastern    Status: Available Reserve

Advanced NGS library prep for challenging samples

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 27, 2016 at 9:30 AM Eastern    Status: Available Reserve
Thursday, October 6, 2016 at 11:00 AM Eastern    Status: Available Reserve
Monday, October 31, 2016 at 1:00 PM Eastern    Status: Available Reserve

Introduction to microRNA

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 19, 2016 at 9:30 AM Eastern    Status: Available Reserve
Tuesday, October 18, 2016 at 1:00 PM Eastern    Status: Available Reserve

Studying the microbiome: tools for microbial detection and host-response analysis

The research community has begun correlating the makeup of individual microbiomes with disorders and diseases such as autism, atherosclerosis, obesity and cancer. To accomplish this, researchers must first identify and characterize these microbial communities. This webinar will provide you with a complete overview of the microbiome, metagenomics and host-pathogen interactions. Experimental strategies to facilitate your microbiome research will be discussed. We will also present a variety of Sample to Insight workflows that integrate MO BIO’s nucleic acid isolation technology with QIAGEN’s microbial qPCR and NGS research tools.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, September 28, 2016 at 1:00 PM Eastern    Status: Available Reserve
Monday, October 31, 2016 at 1:00 PM Eastern    Status: Available Reserve

How to analyze heterogeneous tumor samples?

"Analysis of tumors is already challenging and it becomes more complex when they are heterogeneous. There are a lot of studies suggesting that cells inside tumors are not genetically identical. When tumors are diagnosed clinically, most of them display heterogeneity in their morphological and physiological features. Tumor heterogeneity could be intra or inter-tumor heterogeneity. This heterogeneity within tumors might be responsible for their resistance to therapeutic agents. Tumor heterogeneity can be studied by genotyping or differential gene expression analysis. There are various technologies to study tumor heterogeneity such as qPCR, Sequenome oncocarta, Sanger sequencing and NGS, each with its own pros and cons. Technologies such as single cell sequencing and target enrichment have made it possible to access the single cell genome for sequencing and enable the detection of rare mutations, respectively. Genotypic studies at the single cell level in tumors might yield new therapeutic targets to aid in the fight against the multidrug resistance of cancer. This webinar will provide an overview on tumor heterogeneity, the challenges associated with analyzing heterogeneous tumor samples and the possible solutions."

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 22, 2016 at 1:00 PM Eastern    Status: Available Reserve

CTC Detection and molecular characterisation – Challenges and Solutions

Circulating Tumor Cells (CTCs) have been extensively explored as circulating biomarkers in various cancers. Due to their rarity, heterogeneity and stem cell-like properties, detecting and profiling CTCs from blood samples is very challenging. In this webinar, Dr. Siegfried Hauch will introduce the well-known AdnaTests, which uses the Combination of Combinations Principle (COCP) to enable enriching and detecting CTCs in whole blood with high specificity and sensitivity, and how to overcome challenges in CTC enrichment and detection. The AdnaTests combine an immunomagnetic capturing method that increases purity, and is followed by molecular profiling of the captured CTCs. In addition, leukocyte contamination is another issue in CTCs detection and may lead to false positive results due to illegitimate expression of target genes or false interpretation. The AdnaWash is developed to reduce leukocyte contamination to such a level that whole gene panels can be analyzed while maintaining the required specificity and sensitivity.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, September 9, 2016 at 1:00 PM Eastern    Status: Available Reserve
Wednesday, October 19, 2016 at 9:30 AM Eastern    Status: Available Reserve

RNA-seq for biomarker discovery

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, October 4, 2016 at 9:30 AM Eastern    Status: Available Reserve

Minimize biases in comparing metagenomics with metatranscriptomics: parallel isolation of DNA and RNA from stool sample

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, October 24, 2016 at 9:30 AM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.