See the instructions for the Web Seminars

May 2012
Monday	Tuesday	Wednesday	Thursday	Friday
	1 Lentiviral-Based Pathway Reporter System	2 PCR Arrays for Pathway Analysis	3 Epigenetic Technologies for Biomedical Research PCR Array Data Analysis Tutorial	4 QPCR Introduction
7 Research tools for the innate immune system	8 Cell Death Research Solutions	9 RNAi Screen Design & Optimzation miRNA overview	10 Cignal applications	11 Cancer drug targets
14 Cancer Mutation Analysis	15 Gene Network Central Tutorial	16 PCR Arrays for Pathway Analysis miRNAs expression and quantitation	17 ChIP and Real-Time PCR Technology Overview Signal transduction targets	18 PCR Array Data Analysis Tutorial
21 ELISArray- Analyze Multiple Cytokines and Chemokines	22 Metabolism and cancer	23 Research tools for inflammation regulation miRNAs in Serum	24 Profiling DNA Methylation with PCR System Copy number: variation & alteration analysis strategies	25 Discovery and validation of biomarkers RNAi Gene Knockdown with shRNA
28	29 Cell junction & cell biology research tool	30 Discover miRNA, Proteins and Pathways that Regulate Any Gene	31 miRNA Expression and Function PCR Arrays for Pathway Analysis

Current Seminar Titles Available:

Focus	Title
1.  siRNA	RNAi Screening Experiments: Challenges and Solutions
2.  PCR Array	PCR Array Data Analysis Tutorial
3.  PCR Array	PCR Arrays: The Real Pioneer in Real-Time PCR Analysis of Biological Pathways
4.  Lentiviral-Based Pathway Reporter System	Measure Signaling Pathway Activity in Any Mammalian Cell
5.  Pathway Reporter Assay	Cell-Based Assays for Rapidly Analyzing Pathway Signaling Activity
6.  RNA Interference	Knock Down Your Favorite Genes or Pathways with Ease and Confidence
7.  Multi-Analyte ELISArrays	Analyze 12 Cytokines or Chemokines Simultaneously with ELISArrays
8.  Chromatin IP & Real-Time PCR	Chromatin Immunoprecipitation and Real-Time PCR Technology Overview
9.  DNA Methylation PCR System	Profile the Methylation Status of Multiple Genes Simultaneously Without Bisulfite
10.  SureFIND Transcriptome PCR Arrays	Transcriptome PCR Arrays
11.  Epigenetic Products	New Epigenetic Technologies for Biomedical Research: microRNA, Chromatin IP, and DNA Methylation PCR Arrays
12.  qBiomarker Somatic Mutation PCR Arrays	Cancer Mutation Analysis Made Easy – qBiomarker Somatic Mutation PCR Arrays
13.  miRNA Expression and Function	From Cellular to Molecular - New Assays for miRNAs
14.  QPCR Introduction	Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)
15.  Oxidative Stress Research Solutions	Oxidative Stress: New Research Solutions
16.  Cell Death	Cell Death: New Research Solutions for Apoptosis, Autophagy and Necrosis
17.  PCR Arrays in Biomarker	Discovery & Validation of Biomarkers to Monitor Genotoxicity by Gene Expression Profiling
18.  miRNAs	The miRNA revolution: An introduction to microRNA biogenesis, function & analysis
19.  miRNAs	miRNA Quantitation: From experimental design through data analysis
20.  miRNAs	Profiling miRNA expression in cells, FFPE samples, and serum: on the road to biomarker development.
21.  PCR Arrays in Immunology	Exploring the first line of defense: research tools for the innate immune system
22.  Chromatin Regulation	Chromatin-based regulation of gene expression
23.  Metabolism and cancer	Metabolism and cancer
24.  Copy number analysis	Copy Number: Variation and Alteration Analysis Strategies
25.  Signal targets	Signal Transduction Targets: Research Solutions for Pathway Analysis
26.  Inflammation regulation	InflammaTools: Complete research tools to study Inflammation regulation.
27.  Cell junction & cell biology	What’s Your Cell Line Saying? Cell Junctions & Cell Biology
28.  Anticancer Immunity	Induction and enhancement of anticancer immunity

RNAi Screening Experiments: Challenges and Solutions Genome-wide or large-scale RNA interference (RNAi) screens are complex experiments that require controls and optimization. These screens have inherent rates of both false positives and negatives. QIAGEN has provided an array of RNAi screening solutions that include siRNA design, delivery, and appropriate controls. This 45-minute seminar will cover designing and optimizing RNAi screens, the challenges researchers are facing and solutions to address these challenges. These principles will be presented within the context of two application examples. Come and learn how to use this powerful technique! Duration: 45 minutes followed by Q&A session. Schedule: Friday, June 29, 2012 at 1:00 PM Eastern    Status: Available Reserve

PCR Array Data Analysis Tutorial With a live demonstration using actual PCR array data, learn how easy it is to use our data analysis Web portal to calculate fold-differences in gene expression from your raw real-time PCR threshold cycles. Duration: 45 minutes followed by Q&A session. Schedule: Friday, May 18, 2012 at 1:00 PM Eastern    Status: Available Reserve Thursday, June 7, 2012 at 5:00 PM Eastern    Status: Available Reserve Thursday, June 21, 2012 at 9:30 AM Eastern    Status: Available Reserve

PCR Arrays: The Real Pioneer in Real-Time PCR Analysis of Biological Pathways In this 45-minute Webinar, see how the PCR array has led the way toward simple and accurate real-time PCR analysis of relevant biological pathways and diseases. Learn about the stringent performance built into the technology to ensure its sensitivity, specificity, reproducibility, and reliability. Find out how researchers like you have applied PCR arrays to their cancer, immunology, and toxicology research. Duration: 45 minutes followed by Q&A session. Schedule: Thursday, May 31, 2012 at 5:00 PM Eastern    Status: Available Reserve Thursday, June 14, 2012 at 9:30 AM Eastern    Status: Available Reserve

Measure Signaling Pathway Activity in Any Mammalian Cell Would you like to measure signaling pathway activity in your favorite cell? Now you can! Attend this Webinar to learn about our new breakthrough cellular assay technology. Cignal Lenti Reporter Assays are the newest addition to the Cignal Reporter cell-based assay system. The combined power of a lentiviral delivery system with our robust transcription factor reporter technology enables you to study signal pathways (NF-kB, Wnt, MAPK, hypoxia, Notch, and many more) in virtually any cell type. A technology overview, protocol tutorial, and application examples will be presented. Duration: 45 minutes followed by Q&A session. Schedule: Friday, June 22, 2012 at 1:00 PM Eastern    Status: Available Reserve

Cell-Based Assays for Rapidly Analyzing Pathway Signaling Activity Are you interested in studying the regulation of cell-signaling pathways? Learn how to successfully apply convenient and robust reporter assays to your RNA interference, gene over-expression, protein, or small molecule studies. These assays are an excellent tool for studying pathway signaling activity in cells that are amenable to transfection. Cignal Reporter Assays are available for studying numerous pathways (Wnt, NF-kB, Notch, cAMP/PKA, TGFβ, and many more) relevant to cancer research, immunology, and development. Duration: 45 minutes followed by Q&A session. Schedule: Friday, June 8, 2012 at 1:00 PM Eastern    Status: Available Reserve

Knock Down Your Favorite Genes or Pathways with Ease and Confidence Off-target effects and knockdown efficiency are two major concerns for gene function studies using RNA interference. Learn how our SureSilencing shRNA Plasmids and siRNA arrays address these concerns with rigorous knockdown validation, multiple plasmid-based shRNA designs, and knockdown of genes in the same pathway. Studies focused on cancer research and immunology will be discussed to illustrate the applications of these gene knockdown products. Duration: 45 minutes followed by Q&A session. Schedule: Friday, May 25, 2012 at 1:00 PM Eastern    Status: Available Reserve Tuesday, June 26, 2012 at 9:30 AM Eastern    Status: Available Reserve

Analyze 12 Cytokines or Chemokines Simultaneously with ELISArrays We worked hard to make multi-analyte ELISA easy! Learn how to profile 12 cytokines or chemokines in 6 samples all at the same time with our new Multi-Analyte and Single-Analyte ELISArray technology. The ELISArray Kits are optimized assays for measuring human or mouse cytokine or chemokine expression in cell culture supernatants, serum, or plasma. A technology overview, protocol tutorial, and application data will be presented. Duration: 45 minutes followed by Q&A session. Schedule: Monday, May 21, 2012 at 1:00 PM Eastern    Status: Available Reserve Friday, June 15, 2012 at 1:00 PM Eastern    Status: Available Reserve

Chromatin Immunoprecipitation and Real-Time PCR Technology Overview Interested in studying how specific transcription factors and histone modifications regulate the expression of genes? Learn how to measure in vivo protein and genomic DNA interactions with the proven and quantitative EpiTect® ChIP System for real-time PCR analysis of chromatin immunoprecipitation (ChIP). In this 45-minute Webinar, see how easy a ChIP experiment can really be with our EpiTect ChIP OneDay Kit, how our search portal finds the right PCR primers for your transcription factor and/or gene of interest, and how the system can be applied to your research. Duration: 45 minutes followed by Q&A session. Schedule: Monday, June 11, 2012 at 5:00 PM Eastern    Status: Available Reserve

Profile the Methylation Status of Multiple Genes Simultaneously Without Bisulfite The DNA methylation profiles of promoter CpG islands are important epigenetic biomarkers to both cancer and development research. Traditional bisulfite-based methods are too tedious and time-consuming to effectively keep pace with the needed rate of biomarker discovery and validation. In this 45-minute Webinar, learn about the new EpiTect® Methyl qPCR Arrays, a faster, easier, and higher-throughput DNA methylation profiling method that combines the reliability of well-controlled restriction digestions with quantitative PCR detection. Duration: 45 minutes followed by Q&A session. Schedule: Thursday, May 24, 2012 at 9:30 AM Eastern    Status: Available Reserve Monday, June 18, 2012 at 5:00 PM Eastern    Status: Available Reserve

Transcriptome PCR Arrays Gene expression is regulated by both pre- and post-transcriptional mechanisms. One of the most common challenges for scientists is identifying the molecules that regulate changes in the expression of a gene.  Learn how SureFIND Transcriptome PCR Arrays identify the miRNA, transcription factor, or signaling pathway that regulates a gene’s expression. During this Webinar, you will learn how SureFIND Transcriptome PCR Arrays are carefully designed and how easily you can understand the mechanisms regulating your gene of interest in a single afternoon. The Webinar concludes with an application experiment demonstrating the convenient data analysis software. Come and discover how SureFIND Transcriptome PCR Arrays will accelerate your research. Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, May 30, 2012 at 1:00 PM Eastern    Status: Available Reserve Tuesday, June 26, 2012 at 5:00 PM Eastern    Status: Available Reserve

New Epigenetic Technologies for Biomedical Research: microRNA, Chromatin IP, and DNA Methylation PCR Arrays Epigenetics has grabbed the attention of many researchers by providing new insights into cell differentiation and oncogenesis. These rapidly expanding studies examine how heritable factors not coded within genomic DNA sequences regulate gene expression. Such factors include DNA methylation, chromatin remodeling due to histone modification, and even, in some cases, microRNA. The experimental methods designed to discover and correlate these factors with biological phenotypes have evolved. However, many still suffer from poor sensitivity and reliability, low-throughput, and unnecessary complexity. This Webinar introduces advanced yet easy-to-use real-time PCR array technologies that analyze microRNA as well as mRNA expression profiles, CpG island DNA methylation, and chromatin immunoprecipitation fractions. Find out how easily real-time PCR-based epigenetics experiments can be performed in cancer and stem cell research to better understand the molecular epigenetic mechanisms regulating key genes of interest. Duration: 45 minutes followed by Q&A session. Schedule: Monday, June 4, 2012 at 5:00 PM Eastern    Status: Available Reserve

Cancer Mutation Analysis Made Easy – qBiomarker Somatic Mutation PCR Arrays How can you quickly and accurately characterize somatic mutations associated with cancer signaling pathways? Come join the Webinar to learn about the newest real-time, PCR-based product: qBiomarker Somatic Mutation PCR Arrays. Somatic mutations occur as single or multiple mutations within a gene or a panel of genes associated with tumorigenesis, such as genes in the EGFR, ErbB2, FLT3, and PDGF pathways. Responses to treatment regimens or biological phenotypes may differ based on a cells somatic mutation profile. Each qBiomarker Somatic Mutation PCR Array contains a panel of 5' nuclease assays for a stringently selected set of pathway focused somatic mutations, gene copy number controls, and PCR quality controls. Learn how you can profile the somatic mutation status in your samples with a simple 2-step protocol with the pathway-focused qBiomarker Somatic Mutation PCR Arrays. Duration: 45 minutes followed by Q&A session. Schedule: Thursday, June 28, 2012 at 9:30 AM Eastern    Status: Available Reserve

From Cellular to Molecular - New Assays for miRNAs Understanding the mechanisms used within living cells requires a systematic survey of gene, protein, and pathway activities and how these components interact with one another. In this Webinar, application examples will be presented highlighting various pathway focused technologies. The presented studies will focus on tools for miRNA research including measuring miRNA and gene expression; identifying the effects of miRNAs on cellular signaling, and identifying miRNAs that target a gene-of-interest. Duration: 45 minutes followed by Q&A session. Schedule: Thursday, May 31, 2012 at 9:30 AM Eastern    Status: Available Reserve Monday, June 25, 2012 at 5:00 PM Eastern    Status: Available Reserve

Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR) Real-time polymerase chain reaction (qPCR) is the most sensitive and reliable method for the detection and quantification of nucleic acids. This 45-minute webinar introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that will be covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications, and factors for success. This webinar is for both beginners that are new to real-time PCR as well as experienced users. Duration: 45 minutes followed by Q&A session. Schedule: Friday, June 1, 2012 at 1:00 PM Eastern    Status: Available Reserve

Oxidative Stress: New Research Solutions Oxidative stress is the result of an imbalance in pro-oxidant/antioxidant homeostasis that arises from persistent elevation of Reactive Oxygen Species (ROS) production. Besides endogenous sources of ROS, such as those produced by mitochondria, peroxisomes, and inflammatory cell activation, there exist multiple exogenous sources of ROS, including environmental pollutants, pharmaceutical agents, and industrial toxins. When not counterbalanced by antioxidant defenses of the cell, oxidative stress damages vital cellular macromolecules, including DNA, protein, and lipids.  Oxidative stress disrupts essential cellular functions and is implicated in several human diseases, including cancer, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis.  This webinar will focus on providing a detailed introduction to oxidative stress, outline of the cellular antioxidant defense mechanisms, and current research tools that detect oxidative stress in vitro. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, June 12, 2012 at 1:00 PM Eastern    Status: Available Reserve

Cell Death: New Research Solutions for Apoptosis, Autophagy and Necrosis This webinar will focus on cell death regulation and solutions for studying this process. An introduction to cell death and the hallmark characteristics will be presented. The webinar will discuss the contribution of regulated cell death to various physiological processes. Examples will highlight the role of cell death signaling in development, injury, and the consequences of dysregulated cell death signaling as a cause for diseases such as neurodegenerative diseases, and various cancers. This webinar will introduce state-of-the-art research tools for analyzing cell death and its regulation. Watch and learn how to use these tools in your research model system. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, June 5, 2012 at 1:00 PM Eastern    Status: Available Reserve

Discovery & Validation of Biomarkers to Monitor Genotoxicity by Gene Expression Profiling This webinar will discuss the use of the RT2 Profiler PCR Array System and the miScript miRNA PCR Array System for toxicogenomic biomarker discovery and validation. First, we demonstrate how the RT2 Profiler PCR Array can be used to develop an 11-gene marker panel as a specific and efficient classifier to discriminate genotoxic and non-genotoxic compounds. Then, we show how you can use miScript miRNA PCR Arrays to monitor differential miRNA expression after exposure to a carcinogen, identifying potential candidate miRNAs to serve as genotoxic biomarkers. Join us to discover how gene expression profiling can help find biomarkers in your system! Duration: 45 minutes followed by Q&A session. Schedule: Friday, May 25, 2012 at 11:00 PM Eastern    Status: Available Reserve

The miRNA revolution: An introduction to microRNA biogenesis, function & analysis miRNA plays a critical role in many biological processes such as differentiation and development, cell signaling, response to infection, and other challenges. Recent studies have shown overwhelming evidence that aberration in miRNA expression is causal or an indicator of many disease processes including cancer. Currently there is a race to find and develop miRNAs as biomarkers and therapeutics. To win the race, it is important to have a strong understanding of miRNA and the challenges of miRNA research. The webinar will cover the biology of miRNA, the key challenges associated with miRNA research, and the latest advances in miRNA research technology. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, June 5, 2012 at 9:30 AM Eastern    Status: Available Reserve

miRNA Quantitation: From experimental design through data analysis miRNAs play a critical role in many biological processes including neuronal cell fate, hematology, apoptosis and developmental biology. Increasing evidence has shown that aberrations in microRNA regulation are associated with many disease processes. This webinar will discuss the challenges associated with miRNA quantification by real-time PCR, and the latest strategies for miRNA quantification and profiling. The critical parameters of profiling miRNA gene expression will also be presented. Come and learn how you can apply these tools in your miRNA research! Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, June 12, 2012 at 9:30 AM Eastern    Status: Available Reserve

Profiling miRNA expression in cells, FFPE samples, and serum: on the road to biomarker development. The holy grail of disease diagnosis is to use minimally invasive patient sampling to detect and analyze a stable biomarker with characteristics that are strongly prognostic of a particular disease or condition. Recent studies show overwhelming evidence that miRNAs are a normal component of the extracellular compartment of the blood, and are surprisingly stable in this state. How they get there, or what their normal role might be, remains under intensive investigation. However, multiple reports profiling circulating miRNAs from people with and without disease suggest that particular miRNAs may be ideal biomarkers. This webinar will cover the latest advances in miRNA research tools, as well as strategies and challenges for profiling miRNAs in cells, FFPE tissue, and serum samples.   Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, May 23, 2012 at 4:00 PM Eastern    Status: Available Reserve Tuesday, June 19, 2012 at 9:30 AM Eastern    Status: Available Reserve

Exploring the first line of defense: research tools for the innate immune system The innate immune system executes crucial and unique functions for host defense against infection. This webinar provides an overview of the most important cellular and molecular players of innate immunity and discusses their functions in a variety of disease states. We also introduce research technologies for exploring innate immune activity in your system through profiling of gene expression and cytokines, signal transduction pathway analysis and more, all in the context. Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, June 6, 2012 at 1:00 PM Eastern    Status: Available Reserve

Chromatin-based regulation of gene expression We know that gene expression requires the transcription of DNA sequences into RNA molecules which become translated into the proteins that facilitate biological pathways. But first, transcription factors, RNA polymerase, and other proteins must all be able to bind genomic DNA in the context of chromatin, a complex of DNA with structural DNA-binding proteins known as histones. Post-translational modifications of histones define whether local chromatin regions are in an open or a closed state thereby controlling other proteins’ accessibility to specific genes. Fully understanding the biology behind a phenotype requires analyzing not only the expression of the responsible genes, but also the chromatin-based epigenetic mechanisms regulating that expression. This webinar will review the importance of chromatin status to gene expression and provide examples of how studying both have led to a deeper understanding of biological phenotypes. Duration: 45 minutes followed by Q&A session. Schedule: Monday, June 18, 2012 at 1:00 PM Eastern    Status: Available Reserve

Metabolism and cancer This webinar will focus on providing an introduction to cellular metabolic regulation during oncogenesis and solutions for studying this process. An overview of metabolic pathways (Glycolysis, beta-oxidation, and oxidative phosphorylation) and common pathogenic mechanisms will be presented. Examples will highlight the role of key metabolic steps resulting in carcinogenesis and research for potential therapies. This webinar will introduce state-of-the-art research tools for analyzing the metabolism in cancer and its regulation. Watch and learn how to use these tools in your research model system. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, May 22, 2012 at 1:00 PM Eastern    Status: Available Reserve Wednesday, June 13, 2012 at 1:00 PM Eastern    Status: Available Reserve

Copy Number: Variation and Alteration Analysis Strategies Structural changes in the genome result in either increasing or decreasing the number of copies present for different genomic loci. These changes can occur in the germ line and are heritable or can occur in somatic cells de novo. Alterations in gene copy number have profound effects on human biology and are associated with both developmental disorders and diseases. This webinar will begin with a general introduction of structural genomics followed by a review of historical approaches to measure changes in copy number. The webinar will conclude with an introduction of today’s most advanced techniques for measuring copy number and strategies to improve your genomic and translational studies. Learn how to apply these genomics tools to your research! Duration: 45 minutes followed by Q&A session. Schedule: Thursday, May 24, 2012 at 1:00 PM Eastern    Status: Available Reserve Wednesday, June 13, 2012 at 9:30 AM Eastern    Status: Available Reserve

Signal Transduction Targets: Research Solutions for Pathway Analysis This webinar will focus on providing an introduction to signal transduction pathway analysis and solutions for studying this process. Cellular stimuli provoke cells to change their behavior or characteristics by expressing a different set of genes in a process known as signal transduction. The binding of ligands to their receptor triggers specific signal transduction pathways via protein kinase cascades that activate one or more transcription factors. The activated transcription factors alter the expression of multiple downstream effector genes. Two or more signal transduction pathways often cross-talk or synergize via the activation or inhibition of intracellular signaling molecules in one pathway by those in another. An overview of several common signal transduction pathways (TGFbeta, NFkB, WNT) will be presented. Examples will highlight the role of downstream effector genes in the mechanism of novel biological processes. This webinar will introduce state-of-the-art research tools for analyzing the activation and regulation of signal transduction pathways. Watch and learn how to use these tools in your research model system. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, June 19, 2012 at 1:00 PM Eastern    Status: Available Reserve

InflammaTools: Complete research tools to study Inflammation regulation. This webinar will focus on providing an introduction to the regulation of Inflammation and solutions for studying this process. An overview of Inflammation and key cellular, cytokine, and signaling players will be presented. The webinar will discuss the contribution of Inflammation to various physiological processes. Examples will highlight the role of Inflammation mainly in carcinogenesis and autoimmune diseases. This webinar will introduce state-of-the-art research tools for analyzing Inflammation and its regulation. Watch and learn how to use these tools in your research model system. Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, May 23, 2012 at 1:00 PM Eastern    Status: Available Reserve Wednesday, June 27, 2012 at 1:00 PM Eastern    Status: Available Reserve

What’s Your Cell Line Saying? Cell Junctions & Cell Biology Cell junctions are subcellular macromolecular structures connecting cells to each other or to the extracellular matrix. Each cell junction (whether focal adhesions, tight junctions, gap junctions, adherens junctions, desmosomes, or hemidesmosomes) plays a specific role in cell adhesion, intercellular communication, and intracellular signal transduction. Although specific cell junctions predominate in certain cell types, all cells interact with their environment via more than one or even all of these junctions. How can you determine the relative importance of each cell junction to the cellular or disease process that you study in your cell line of interest? Analyzing the expression of their components is a good place to start. This webinar will review the function of cell junctions in cell biology and provide examples of how gene expression analysis helps us better understand their role in biological processes and phenotypes. Duration: 45 minutes followed by Q&A session. Schedule: Tuesday, May 29, 2012 at 1:00 PM Eastern    Status: Available Reserve

Induction and enhancement of anticancer immunity This webinar will review the newly discovered mechanisms of anticancer immune response, which include immune checkpoints and the cross-talk between cancer cells and immune cells. Research and therapeutic strategies will be presented. Join us to discover how these solutions can be used to facilitate your cancer research. Duration: 45 minutes followed by Q&A session. Schedule: Wednesday, June 20, 2012 at 1:00 PM Eastern    Status: Available Reserve