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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

February 2016
Monday
Tuesday
Wednesday
Thursday
Friday

1

Onestep ahead RT-PCR

2

QPCR Introduction

3

miRNA overview

4

Host–pathogen interactions

5

8

Profiling miRNA from single cells

9

Coding & noncoding RNA analysis using qPCR

10

Functional analysis of miRNAs

11

Enrich microbial DNA with our protocol

NGS in mutation identification of cell-free circulating tumor DNA

12

15

long noncoding RNA (lncRNA)

gDNA collection, storage, and purification

16

PCR Array Data Analysis Tutorial

Single cell analysis: sample to insight

17

Introduction to RNAseq

Practical hints for successful PCR

18

Microbiome: From identification to characterization

Clinical sequencing for meningiomas

19

long noncoding RNA (lncRNA)

Quality control of DNA - tips & tricks

22

Oxidative stress in aging and diseases

23

Service for biomarker research

Analysis of Single-Cell Sequencing Data

24

RNAseq targeted panels

Controls and novel solutions for real-time qPCR

25

long noncoding RNA (lncRNA)

26

Target NGS for cancer research

29

cfDNA handling and purification

Current Seminar Titles Available:

Focus Title
1.  PCR ArrayPCR Array Data Analysis Tutorial
2.  QPCR IntroductionIntroduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)
3.  miRNAsThe miRNA revolution: An introduction to microRNA biogenesis, function & analysis
4.  PCR Arrays in ImmunologyExploring the first line of defense: research tools for the innate immune system
5.  Inflammation and TLRToll-like Receptors in Inflammation
6.  miRNAsFunctional analysis of miRNAs
7.  Next-generation sequencingNext-generation sequencing, an introduction to technology and applications
8.  Microbial PCR ArraysMicrobiome: From identification to characterization
9.  Host–Pathogen InteractionsHost–Pathogen Interactions: Molecular Basis and Host Defense Mechanisms
10.  miScript miRNA for plantsPlant miRNAs: New focused genomic solutions
11.  lncRNANew advances in long noncoding RNA (lncRNA) research
12.  Next-generation sequencingNext-Generation Sequencing — Targeted Enrichment Technology in Cancer Research
13.  Next-generation sequencingAdvancing NGS Data Analysis and Interpretation: CLC Cancer Research Workbench and Ingenuity Variant Analysis
14.  Single cell analysisSingle cell analysis - from sample to insight
15.  Service CoreAccelerate your discovery with QIAGEN service solutions for biomarker research
16.  Multiplex PCRPractical hints and new solutions for successful real-time PCR studies
17.  PCR ArraysAdvanced Real-Time PCR Array Technology – Coding & Noncoding RNA Expression Analysis
18.  Single cell analysisAnalysis of Single-Cell Sequencing Data by CLC / Ingenuity
19.  DNA sample isolationFundamental Concepts and Special Considerations in gDNA Isolation for Better Insight
20.  DNA sample isolationChallenges of FFPE Sample Materials – Where Does Variation in Quantity of Purified DNA Come From?
21.  Oxidative stress and agingOxidative Stress in Aging and Human Diseases – Exploring the Mechanisms
22.  Onestep ahead RT-PCROne step ahead for your RT-PCR
23.  Next-generation sequencingDeveloping a rapid clinical sequencing system to classify meningioma
24.  Microbiome researchMicrobiome: isolate and enrich microbial DNA with our new protocol
25.  RNAseq technologyDigital RNASeq: Technology introduction
26.  RNAseq technologyDigital RNAseq for gene expression profiling: Workflow and applications
27.  RNAseq technologyMolecular insight into gene expression using Digital RNAseq: Data analysis tutorial
28.  QuantiNova PCR kit The importance of controls and novel solutions for successful real-time qPCR
29.  Liquid BiopsiesNew Technology and workflow for integrated collection, stabilization, and purification of circulating cell-free DNA
30.  Liquid BiopsiesAnalysis and interpretation of cell free DNA
31.  Single cell analysisAdvances and applications enabled by single-cell technology
32.  Ingenuity Pathway Analysis (IPA) Interpret Your Gene Expression Analysis Results with Advanced Bioinformatics Tools
33.  Adaptive immunityStudy the Adaptive Immune Response: Tools for T and B cell Research
34.  Cancer immune responseThe Crosstalk between Cancer Inflammation and Immunity – Exploring the cancer immune responses
35.  Liquid BiopsiesLiquid biopsy: overcome challenges of circulating DNA with automated and standardized extraction processes

PCR Array Data Analysis Tutorial

With a live demonstration using actual PCR array data, learn how easy it is to use our data analysis Web portal to calculate fold-differences in gene expression from your raw real-time PCR threshold cycles.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, February 16, 2016 at 9:30 AM Eastern    Status: Available Reserve
Thursday, March 17, 2016 at 9:30 AM Eastern    Status: Available Reserve

Introduction to Real Time PCR (Q-PCR/qPCR/qrt-PCR)

Real-time polymerase chain reaction (qPCR) is the most sensitive and reliable method for the detection and quantification of nucleic acids. This 45-minute webinar introduces the concepts of real-time PCR and how to conduct a real-time PCR assay. The topics that will be covered include an overview of real-time PCR chemistries, protocols, quantification methods, real-time PCR applications, and factors for success. This webinar is for both beginners that are new to real-time PCR as well as experienced users.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, March 3, 2016 at 9:30 AM Eastern    Status: Available Reserve

The miRNA revolution: An introduction to microRNA biogenesis, function & analysis

miRNA plays a critical role in many biological processes such as differentiation and development, cell signaling, response to infection, and other challenges. Recent studies have shown overwhelming evidence that aberration in miRNA expression is causal or an indicator of many disease processes including cancer. Currently there is a race to find and develop miRNAs as biomarkers and therapeutics. To win the race, it is important to have a strong understanding of miRNA and the challenges of miRNA research. The webinar will cover the biology of miRNA, the key challenges associated with miRNA research, and the latest advances in miRNA research technology.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 2, 2016 at 1:00 PM Eastern    Status: Available Reserve

Exploring the first line of defense: research tools for the innate immune system

The innate immune system executes crucial and unique functions for host defense against infection. This webinar provides an overview of the most important cellular and molecular players of innate immunity and discusses their functions in a variety of disease states. We also introduce research technologies for exploring innate immune activity in your system through profiling of gene expression and cytokines, signal transduction pathway analysis and more, all in the context.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 8, 2016 at 9:30 AM Eastern    Status: Available Reserve

Toll-like Receptors in Inflammation

"Toll-like receptors (TLRs) have been implicated in both innate and adaptive immunity-induced inflammation, thereby playing critical roles in providing the host with short- and long-term protection against infections.. This webinar will provide an overview of the roles that TLRs play in the regulation of Inflammation and solutions for studying these roles. An overview of TLR-mediated inflammation, the key signaling players involved in TLR-mediated inflammation, and the contribution of TLR-mediated inflammation to various physiological processes will be presented. Please come and learn about the state-of-the-art research tools for analyzing TLR-mediated inflammation and its regulation. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 15, 2016 at 9:30 AM Eastern    Status: Available Reserve

Functional analysis of miRNAs

Understanding the function and targets of miRNA is important, but challenging research. Since one miRNA can potentially target several genes, finding and validating a specific miRNA-mRNA interaction often involves multiple molecular techniques. In this webinar we will highlight the use of miRNA mimics, inhibitors and target protectors to increase, decrease and adjust the cellular concentration of miRNA and disrupt specific miRNA-mRNA interactions. We will also present a ready-to-use screening tool for identifying miRNA targets in your samples. Lastly, we will show how miRNA expression data can be used to predict mRNA targets and how qPCR can be used to validate predicted interactions.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 9, 2016 at 1:00 PM Eastern    Status: Available Reserve

Next-generation sequencing, an introduction to technology and applications

Next-generation sequencing (NGS) technology has revolutionized the study of the genomes, transcriptomes and epigenomes of any species. NGS employs massively parallel sequencing to poduce millions of sequences at once and delivering fast, inexpensive, and accurate genome information. This webinar will provide a technical overview of DNA/RNA preprocessing, template preparation, sequencing, and data analysis. It will also cover the applications for NGS technologies, including guidelines for how to select the technology that will best address your biological question of interest.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 8, 2016 at 1:00 PM Eastern    Status: Available Reserve

Microbiome: From identification to characterization

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, February 18, 2016 at 9:30 AM Eastern    Status: Available Reserve
Thursday, March 17, 2016 at 1:00 PM Eastern    Status: Available Reserve

Host–Pathogen Interactions: Molecular Basis and Host Defense Mechanisms

Host–pathogen interactions are strikingly complex during infection. This webinar provides an overview of the molecular basis of these intricate interactions; the impact of microbiota on innate and adaptive immunity, metabolism, and insulin resistance; and host defense mechanisms, including newly reported inflammasome-mediated responses and miRNA-mediated responses. Various research tools will be introduced to simplify and streamline each step of studying the host response, enabling analysis of gene expression and regulation, epigenetic modification, genotyping, and signal transduction pathway activation.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, March 3, 2016 at 1:00 PM Eastern    Status: Available Reserve

Plant miRNAs: New focused genomic solutions

Plant miRNAs play a necessary role in processes such as development, disease resistance and stress response. These small non-coding RNAs are thought to regulate gene expression in plants at the post-transcriptional level by targeting mRNAs for cleavage. These targeted alterations in gene expression are mediated by differential expression of hundreds of mature miRNAs that each bind to specific sequences in the target mRNAs. Therefore, profiling the coordinate regulation and expression of mature plant miRNAs provides invaluable insight into mechanisms that an organism is using to develop tissue, protect itself from drought or some disease. Until now, universal profiling of mature plant miRNAs by qRT-PCR was virtually non-existent because polyadenylation-based reverse transcription reaction occur with very low efficiency because of the biochemical nature of plant miRNAs. QIAGEN has released a unique solution that allows universal reverse transcription of all mature plant miRNAs in a tissue.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 16, 2016 at 1:00 PM Eastern    Status: Available Reserve

New advances in long noncoding RNA (lncRNA) research

"Recent improvements in RNA-sequencing have led to the discovery of thousands of RNAs that do not contain any open reading frames, termed “long noncoding RNA (lncRNA)”. lncRNA expression is thought to be important for the regulation of gene (mRNA) expression through several different mechanisms, some involving competition with transcription and others involving the recruitment of specific epigenetic factors to promoter regions. lncRNA may also indirectly affect gene expression by interacting with miRNA and other cellular factors. In this webinar, we will review what is known about lncRNA and use application examples in cancer research to illustrate how expression profiling is being used to identify lncRNA biomarkers from cells, tissues, blood, and exosomes. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, February 15, 2016 at 9:30 AM Eastern    Status: Available Reserve
Friday, February 19, 2016 at 11:00 AM Eastern    Status: Available Reserve
Thursday, February 25, 2016 at 1:00 PM Eastern    Status: Available Reserve

Next-Generation Sequencing — Targeted Enrichment Technology in Cancer Research

Genetic variance analysis plays a critical role cancer diagnosis, prognosis and treatment of cancer. Low sample volume and poor DNA quality are nagging issues with cancer samples making the results unreliable and expensive. This webinar discusses the most biologically efficient, cost-effective method for successful NGS. The GeneRead DNA QuantiMIZE Kits enable determination of the optimum conditions for targeted enrichment of DNA isolated from biological samples, while the GeneRead DNAseq Panels V2 allow you to quickly and reliably deep sequence your genes of interest. Applications in translational and clinical research will be highlighted.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, February 26, 2016 at 11:00 AM Eastern    Status: Available Reserve
Monday, March 14, 2016 at 9:30 AM Eastern    Status: Available Reserve
Tuesday, March 15, 2016 at 1:00 PM Eastern    Status: Available Reserve

Advancing NGS Data Analysis and Interpretation: CLC Cancer Research Workbench and Ingenuity Variant Analysis

Deep-sequencing technologies are enabling critical insights into all aspects of cancer genomics. This webinar will discuss two comprehensive informatics solutions — the CLC Cancer Research Workbench and Ingenuity Knowledge Base Variant Analysis platforms. These NGS analysis platforms provide a user-friendly and customizable solution, enabling you to easily narrow down your focus to causal variants and detect and annotate low-frequency somatic variants. We will show the intuitive user interface of CLC Cancer Research Workbench and demonstrate how the rich biological content from Ingenuity Knowledge Base helps you rapidly identify critical variants in your samples.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 22, 2016 at 1:00 PM Eastern    Status: Available Reserve

Single cell analysis - from sample to insight

What can you do from a single cell? Actually, a lot! Beginning with the genome, you can discover new biomarkers by identifying new genetic variances and their association with specific diseases, including cancers. Moving on to RNA, the recent advances in RNA sequencing technology have made single-cell transcriptomics a possibility. Along with these possibilities come challenges that start from the moment you get the sample to the final step of gaining insights into the cell. This webinar will provide an overview on the multiple steps involved as you move from sample acquisition to analysis and data interpretation in different sample types.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, February 16, 2016 at 1:00 PM Eastern    Status: Available Reserve

Accelerate your discovery with QIAGEN service solutions for biomarker research

Development of preclinical biomarkers can have several pitfalls, often requiring a large investment in instruments to process samples, time to develop assays, and specialties in data analysis and biomarker algorithm design. QIAGEN’s life sciences service core can provide researchers with the tools, knowledge and specialties to help develop the next generation of biomarkers for cancer, toxicology and inflammation research. This seminar will highlight QIAGEN’s service capabilities in sample isolation, microarray and NGS-sequencing, qPCR panel, and custom assay development and bioinformatics as we look at the identification of potential biomarkers and gene signatures. We will discuss the applications of QIAGEN Service Core in microRNA discovery for toxicology markers in serum and plasma and in identification of RNA signatures for tumor stratification. Join us and learn how you can accelerate your research with QIAGEN service solutions.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, February 23, 2016 at 9:30 AM Eastern    Status: Available Reserve
Thursday, March 24, 2016 at 9:30 AM Eastern    Status: Available Reserve

Practical hints and new solutions for successful real-time PCR studies

In this webinar we will cover the following topics which are critical steps for efficient and precise gene expression studies using real-time PCR technology: - Effect of RNA integrity on real-time PCR results – tips to achieve a true RNA profiling suitable for real-time PCR studies - Improved methods for cDNA synthesis, optimized for real-time PCR - Real-time PCR analysis • Real-time PCR essentials and background information on different quantification strategies • SYBR Green real-time PCR – factors influencing specificity • Introduction to probe technology • New, fast and efficient real-time PCR solutions

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, February 17, 2016 at 9:30 AM Eastern    Status: Available Reserve
Monday, March 7, 2016 at 1:00 PM Eastern    Status: Available Reserve

Advanced Real-Time PCR Array Technology – Coding & Noncoding RNA Expression Analysis

This webinar presents a simple and accurate real-time PCR system for relevant biological pathway- & disease-focused mRNA and long non-coding RNA (lncRNA) expression profiling. Learn about the stringent performance built into the technology to insure its sensitivity, specificity, reproducibility, and reliability. Application examples will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, March 10, 2016 at 9:30 AM Eastern    Status: Available Reserve

Analysis of Single-Cell Sequencing Data by CLC / Ingenuity

"Single-cell analysis is useful to study genetic heterogeneity between individual cells and can help in result interpretation by looking at the average behavior of a large number of cells. Applications include circulating tumor cells, cells from small biopsies and cells from in vitro fertilized embryos. In this webinar, we will show how single-cell next-generation sequencing data can be analyzed and what challenges needs to be overcome. One of the examples we will use is single cell data from two colorectal cancer cell lines. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, February 23, 2016 at 1:00 PM Eastern    Status: Available Reserve

Fundamental Concepts and Special Considerations in gDNA Isolation for Better Insight

"How are your gDNA yields? Some sample types present special challenges in DNA purification and analysis. This webinar provides tips for a whole range of sample types that require special consideration. We will cover the following topics in detail: • The basic methods and challenges of gDNA purification • Working with DNA: good laboratory practice • Special considerations for challenging sample types • Performing DNA quality checks"

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, February 15, 2016 at 1:00 PM Eastern    Status: Available Reserve

Challenges of FFPE Sample Materials – Where Does Variation in Quantity of Purified DNA Come From?

In this live webinar, we will discuss variability in quantity and purity of DNA purified from FFPE samples manually or with automated procedures, assessed by different quantification and quality control methods. We've carried out a comprehensive analysis, so register today and see the results!

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, February 19, 2016 at 1:00 PM Eastern    Status: Available Reserve

Oxidative Stress in Aging and Human Diseases – Exploring the Mechanisms

Many modern diseases, including cancer, cardiovascular disease, diabetes, liver disease, arthritis and neurodegenerative disease, are related to aging, and aging is closely linked to oxidative stress. Intensive research is being conducted to understand the antioxidant defense mechanism, the mechanisms of aging itself, as well as their roles in human diseases. This webinar provides an update on how oxidative stress is linked to aging and how inflammation leads to aging through DNA damage, telomere dysfunction, cellular senescence and oxidative stress. We will also discuss recent progress on the health benefits of antioxidants and examine their potential mechanisms in the prevention and treatment of chronic diseases. Various assay technologies to tackle the complex signaling pathways in this process will be introduced. Please join us to learn how you can apply these advanced tools to your research!

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, February 22, 2016 at 9:30 AM Eastern    Status: Available Reserve

One step ahead for your RT-PCR

"At the heart of every successful discovery lie the seeds of innovation. At QIAGEN, we are constantly developing new methods that allow researchers to gain forward momentum with their research. Whether you’re studying gene expression or performing viral RNA analysis, the success of your experiment depends on the ability to analyze your sample with the highest standards of sensitivity and specificity so that you can have confidence in your data. To help you generate valuable insights from gene expression profiling and viral RNA analysis, we introduce the brand new QIAGEN OneStep Ahead RT-PCR Kit – the first hot-start reverse transcriptase kit on the market. Continuing the success story of its first- generation predecessor (QIAGEN OneStep RT-PCR Kit), the QIAGEN OneStep Ahead RT-PCR Kit is equipped with compelling new features that afford maximum convenience and ease of use, while delivering unmatched sensitivity and specificity. With a total reaction time of 1 hour, higher sequence accuracy and the ability to amplify amplicons of up to 4 kb without tedious optimization, you can get one step closer to publishing your findings with this new solution. For increased convenience, the kit comes in an all-in-one tube format along with a built-in pipetting control. Stay one step ahead of your peers and make significant advances in your research with the QIAGEN OneStep Ahead RT-PCR Kit! In this webinar we will introduce the new kit in detail and discuss its features and benefits. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, March 11, 2016 at 2:00 AM Eastern    Status: Available Reserve

Developing a rapid clinical sequencing system to classify meningioma

Meningioma’s display a broad spectrum of clinical, histological and cytogenetic features even within the same WHO grade often posing a challenge for classification and prognostic stratification. In this webinar, we will describe our experience of using targeted amplicon sequencing to develop rapid clinical sequencing system to identify and confirm the meningioma genotype in just 7 days. In addition the details of the three meningioma categories and the genes involved will be discussed.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, February 18, 2016 at 6:00 PM Eastern    Status: Available Reserve

Microbiome: isolate and enrich microbial DNA with our new protocol

Human microbiome projects have delivered our first glimpse into microbial communities that reside in and on our bodies. However, distinguishing microbial DNA from host DNA remains one of the most problematic challenges facing researchers today. We have developed an easy-to-use workflow that allows selective isolation of microbial DNA from samples that are intrinsically rich in host DNA. This protocol includes steps for efficient depletion of host DNA while providing optimized conditions specific for bacterial lysis. This workflow is also specific for the identification of live bacteria, avoiding false results due to nucleic acids from dead bacteria. Enriched microbial DNA can be directly used in other molecular methods such as whole genome sequencing, qPCR and microarray assays.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, March 10, 2016 at 1:00 PM Eastern    Status: Available Reserve

Digital RNASeq: Technology introduction

QIAseq RNA is a revolutionary turnkey solution for digital gene expression analysis by NGS. From 10 genes to 1000, from one sample to 100, QIAseq RNA delivers precise results on both ION and Illumina sequencing platforms. The data from QIAseq RNA is directly comparable to expression analysis derived from whole transcriptome sequencing or by qRTPCR, only better, cheaper, faster and more flexible. This webinar will describe the principles of digital expression analysis by NGS, and review the features and benefits of the QIAseq system, options available and the integrated data analysis package.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, February 17, 2016 at 1:00 PM Eastern    Status: Available Reserve

Digital RNAseq for gene expression profiling: Workflow and applications

Traditional RNA sequencing (RNA-Seq) is a powerful tool for expression profiling, but is hindered by PCR amplification bias and inaccuracy at low expressing genes. QIAseq RNA is a flexible and precise tool developed for mitigating these complications, allowing digital gene expression analysis. This in-depth webinar will cover sample requirements, experimental design, NGS platform-specific challenges and workflow for gene enrichment, library prep and sequencing. The applications of QIASeq RNA Panels in cancer research, stem cell differentiation and elucidating the effects small molecules on signaling pathways will be highlighted.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, February 24, 2016 at 1:00 PM Eastern    Status: Available Reserve
Friday, March 25, 2016 at 11:00 AM Eastern    Status: Available Reserve

Molecular insight into gene expression using Digital RNAseq: Data analysis tutorial

Gene expression profiling is the key to understanding biological pathways and complex cellular systems. In this webinar, we will discuss the challenges of targeted RNA-seq data analysis and present the solutions provided by the QIAGEN automated online data analysis tools. Using raw sequencing data from targeted sequencing, the output of the QIAseq primary data analysis tool and the options in QIAseq secondary analysis, such as normalization strategies, will be described. The use of Ingenuity Pathway Analysis (IPA) to unlock the molecular insights buried in experimental data by quickly identifying relationships, mechanisms, functions, and pathways of relevance will be shown with an example.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 1, 2016 at 1:00 PM Eastern    Status: Available Reserve

The importance of controls and novel solutions for successful real-time qPCR

"The increasing demand for streamlined, monitored, and ultrafast qPCR procedures requires high-performance, real-time quantitative RT and PCR chemistries. Particularly procedures utilizing generic kits for gene expression analysis should include in-process safety measures to avoid variables and control accuracy of procedures and results. This webinar presents innovative solutions for one-step and two-step RT-PCR that significantly enhance performance and reliability in qRT-PCR. The new QuantiNova kit family offers a combination of various integrated safety features to remove variables and prevent artifacts. Internal Control RNA, removal of genomic DNA, room temperature set up capability for RT-PCR, and a built-in visual pipetting control verify accurate procedures, ensuring reliable gene expression profiling. This webinar explains the principles of the technologies and shows data demonstrating performance in qRT-PCR. Find out how you can verify accurate performance in qRT-PCR and improve your results."

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, February 24, 2016 at 9:30 AM Eastern    Status: Available Reserve
Monday, March 14, 2016 at 1:00 PM Eastern    Status: Available Reserve

New Technology and workflow for integrated collection, stabilization, and purification of circulating cell-free DNA

Research into non-invasive prenatal testing (NIPT) and circulating tumor DNA (ctDNA) testing based on circulating cell-free DNA (ccfDNA) is rapidly expanding. However, detection and quantification of ccfDNA is compromised by the release of genomic DNA (gDNA) from lymphocytes due to mechanical lysis or apoptosis during blood collection, storage and transport. PreAnalytiX has developed the PAXgene® Blood ccfDNA System, consisting of the PAXgene Blood ccfDNA Tube, a plastic blood collection tube with a unique, non-crosslinking chemistry that preserves extracellular levels of ccfDNA and prevents the release of intracellular DNA from cells into the plasma, and the QIAsymphony® PAXgene Blood ccfDNA Kit for automated ccfDNA extraction from up to 5 ml of plasma. In this webinar, this new technology development will be presented in comparison to other existing technologies.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, February 29, 2016 at 9:30 AM Eastern    Status: Available Reserve

Analysis and interpretation of cell free DNA

Identification and monitoring of cancer mutations from cell free DNA-Seq data is a key application in liquid biopsy. In this part of the webinar we will show how mutations can be best identified from this type of data and how they can be interpreted. Furthermore, potential challenges when analyzing this type of data will be discussed together with relevant strategies.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, March 21, 2016 at 9:30 AM Eastern    Status: Available Reserve

Advances and applications enabled by single-cell technology

Over the past 5 years, single-cell genomics have become a powerful technology for studying small samples and rare cells, and for dissecting complex populations such as heterogeneous tumors. Single-cell technology is enabling many new insights into diverse research areas from oncology, immunology and microbiology to neuroscience, stem cell and developmental biology. This webinar introduces single-cell technology and summarizes the newest scientific applications in various research areas, all in the context of current literature.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 30, 2016 at 9:30 AM Eastern    Status: Available Reserve

Interpret Your Gene Expression Analysis Results with Advanced Bioinformatics Tools

"You’ve got raw Ct data and fold changes from your gene expression analysis experiment. How do you make sense of the results to build a model that explains the biology and helps you design the next best experiment? In this webinar, we will introduce a gene expression workflow and an easy-to-use yet advanced Ingenuity Pathway Analysis (IPA) bioinformatics tool. With a case study, we will explore the potential that RT-PCR array and bioinformatics combined to help understanding the regulatory roles of some genes in diseases and get better insight into the biological function and find potential biomarkers. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, March 2, 2016 at 9:30 AM Eastern    Status: Available Reserve
Monday, March 21, 2016 at 1:00 PM Eastern    Status: Available Reserve

Study the Adaptive Immune Response: Tools for T and B cell Research

Adaptive immunity, powered by T cells and B cells, provides specific, long-lasting protection of the host from harmful invaders. This webinar will provide an overview of T cells and B cells and their role in cell-mediated immune responses and antibody responses, respectively, against pathogens. We will explore tools that enable analysis of T and B cell gene expression and regulation, genotyping and signal transduction pathway activation.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 22, 2016 at 9:30 AM Eastern    Status: Available Reserve

The Crosstalk between Cancer Inflammation and Immunity – Exploring the cancer immune responses

This webinar will review the mechanisms of anticancer immune responses, which include immune checkpoints and the cross-talk between cancer cells and the cellular mediators of inflammation and immunity. The impact of gut microbiota in eliciting the immune responses against cancer and modulating the effects of drugs will also be discussed. In addition, we will discuss the roles of long non-coding RNAs (lncRNAs) in cancer progression and immune responses. Research tools and therapeutic strategies will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, March 29, 2016 at 9:30 AM Eastern    Status: Available Reserve

Liquid biopsy: overcome challenges of circulating DNA with automated and standardized extraction processes

"Circulating, cell-free DNA (cfDNA) originating from malignant tumors, a developing fetus and also from inflammatory tissues, is present in the cell-free nucleic acids in plasma, serum and other body fluids and is considered a “liquid biopsy”. Access to cfDNA for analysis allows for specific detection of certain disease states based on a simple blood sample. Circulating cell-free DNA shows distinctive properties – it is present mostly as shorter fragments of less than 500 bp and the concentration of cfDNA in a plasma or serum sample is low (approximately 1–100 ng/ml) compared to cellular materials and varies considerably between different individuals. Because of their fragmented nature and low concentration, cfDNA presents a particular challenge for efficient extraction / purification and quantification, such as by qPCR. We present data on solutions for the following critical problems concerning the purification of cfDNA for research and molecular diagnostic applications: • Pre-analytical workflow (blood processing) for analyzing cfDNA • Optimization of cfDNA extraction from plasma samples: low target concentrations require efficient cfDNA enrichment from larger sample volumes • Novel automated extraction of cfDNA using the QIAsymphony SP instrument for liquid biopsy diagnostic applications "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, March 7, 2016 at 9:30 AM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.