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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

June 2017
Monday
Tuesday
Wednesday
Thursday
Friday

1

QIAscout and AdnaTest for CTCs

2

5

New QIAamp and QS EZ1 ccfDNA kits

6

7

CLC Genomics Workbench: Introduction

CLC Genomics Workbench: Introduction

8

9

12

NGS in hematologic malignancies and solid tumors

QIAseq library kits for ccfDNA sequencing

13

CLC Microbial Genomics for TB case study

14

CLC Genomics Workbench: Variant analysis

CLC Genomics Workbench: Variant analysis

15

Characterizing the microbiome of neonates and infants

16

19

ccfDNA workflow on QIAsymphony

Introduction to miRNA-seq

20

Targeted sequencing in cancer clinical research

21

CLC Genomics Workbench: RNA-seq

CLC Genomics Workbench: RNA-seq

22

Basics of sample prep in RNA research

23

26

miRNA-seq in liquid biopsy

QIAseq NGS for low input WGS

27

Introduction to epigenetics

GeneReader

28

QIAseq NGS for low input WGS

CLC Genomics Cloud Engine

CLC Genomics Cloud Engine

29

RNA integrity and quality

30

Current Seminar Titles Available:

Focus Title
1.  RNA isolationRNA Integrity and Quality – Standardize RNA Quality Control
2.  DNA MethylationDNA Methylation – an Essential Element in Epigenetics Facts, Technologies and Stories
3.  QIAseq miRNA Library KitIntroduction to miRNA-seq using unique molecular indices and gel-free library construction
4.  QIAseq miRNA Library KitmiRNA-seq from liquid biopsy: robust detection from the lowest sample amounts
5.  Ingenuity Pathway Analysis (IPA)Introduction to IPA and the powerful knowledge base behind it
6.  CLC Genomics WorkbenchIntroduction to the CLC Genomics Workbench: A preview
7.  QIAseq NGS Library KitQIAseq Technologies for Low Input Whole Genome Sequencing
8.  QIAseq NGSDigital sequencing: Introduction to Unique Molecular Index (UMI) technology for targeted sequencing
9.  QIAseq NGSTargeted DNA-seq for mutation detection
10.  QIAseq NGSGene expression analysis using targeted RNA-seq and desktop NGS instruments
11.  QIAseq NGSLinking miRNA and gene expression using digital NGS
12.  QIAseq NGSNext-generation sequencing in single-cell applications
13.  CLC Genomics Cloud Engine Introduction to the CLC Genomics Cloud Engine – Enterprise NGS Made Easy
14.  NGS Live in 30 Days (GeneReader)NGS Live in 30 Days: Challenges of, and a solution to, implementing NGS
15.  Bioinformatics for nonbioinformaticiansBioinformatics for non-bioinformaticians in cancer clinical research
16.  NGS: Now integrated and connected with your lab operationsNGS: Now integrated and connected with your lab operations
17.  PyroMark Q48 Autoprep DNA methylation analysis in a single day – the new PyroMark Q48 Autoprep
18.  QIAseq NGS"QIAGEN Wet-Lab Scientist Series: Advice Prior to Using QIAseq Targeted Panels
19.  QIAseq NGSQIAGEN Wet-Lab Scientist Series: Advice for Sequencer Setup & Sequencer Performance Review of QIAseq Targeted Panels
20.  BioinformaticsQIAGEN Wet-Lab Scientist Series: Advice for Data Analysis of QIAseq Targeted Panels
21.  QuantiFERONAlgorithms in TB testing at Scripps Mercy Hospital – effectively managing TB Testing results

RNA Integrity and Quality – Standardize RNA Quality Control

RNA integrity and quality are critical to obtain meaningful and reliable downstream data. This webinar discusses the challenges and considerations of handling RNA samples, the need for quality control analysis and common methods for RNA integrity and quality assessment. The QIAxcel Advanced System will be introduced to automate the process of RNA sample integrity analysis and obtain objective quality measurement. Application data will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, June 29, 2017 at 9:30 AM Eastern    Status: Available Reserve

DNA Methylation – an Essential Element in Epigenetics Facts, Technologies and Stories

"DNA methylation forms one of the multiple layers of epigenetic mechanisms controlling and modulating gene expression. It closely interacts with histone modifications and chromatin-remodeling complexes to form the genomic chromatin landscape. DNA methylation is essential for mammalian development, imprinting and plays a role in maintaining genomic stability, as well as in dosage compensation. Aberrant DNA methylation has been detected in a number of diseases, such as cancer, neurodevelopmental diseases or autoimmune diseases. This webinar • Discusses the different forms of cytosine methylation • Explains the role of DNA methylation as a regulator of gene expression • Illustrates the implications of DNA methylation in diseases • Unravels the methods for analyzing DNA methylation"

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, June 27, 2017 at 9:30 AM Eastern    Status: Available Reserve

Introduction to miRNA-seq using unique molecular indices and gel-free library construction

"In this webinar, we will introduce the newest NGS solution from QIAGEN, the QIAseq miRNA Library Kit. miRNA sequencing has the potential to uncover new miRNAs, identify processing intermediates and also quantitative differences between samples. However, challenges such as FFPE and serum/plasma samples, the need for high amounts of sample input and tedious workflows using size selection electrophoresis has led to disappointing and often irreproducible results. QIAGEN has developed a revolutionary new miRNA sequencing kit to remove these limitations for miRNA-seq. Our kit ensures highly reproducible and cost-effective miRNA-seq – regardless of the sample. Whether you’re working with serum, cells, tissues or FFPE samples, proprietary technology provided by the QIAseq miRNA Library Kit ensures removal of adapter-based dimers, which allows you to achieve higher levels of usable miRNA mapped reads. In addition, the kit allows a completely gel-free workflow for maximum convenience, starting with only 1 ng of total RNA. The QIAseq miRNA Library Kit includes a free online data analysis portal for mapping reads and interpreting the unique molecular indices (UMI), allowing you to obtain the most unbiased and highest-fidelity results possible. Join us for this webinar to discover what you’ve been missing in your miRNA-seq experiments. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, July 11, 2017 at 1:00 PM Eastern    Status: Available Reserve

miRNA-seq from liquid biopsy: robust detection from the lowest sample amounts

miRNAs impact virtually all areas of biology, and in circulation, they are promising biomarker candidates for both normal and disease biology. miRNAs are protected from degradation in virtually all biofluids by exosomes, Ago2, HDL or other protective proteins, but are expressed at low levels. As a result, expression analysis, particularly using next-generation sequencing (NGS), has proven to be difficult. Traditional small RNA library kits lack the sensitivity or specificity to adequately assess miRNA expression. Libraries prepared with using these kits have been fraught with background products, such as adapter dimers and other RNAs, including hY4 Y RNA. These problems collectively manifest as a low mapping percentage to miRNA, a limited dynamic range and lost discovery potential. QIAGEN’s QIAseq miRNA Library Kit is specifically designed to overcome these challenges. The innovative, gel-free workflow enables the preparation of robust libraries from even the most difficult, low RNA content biofluids. QIAseq miRNA maximizes your on-target miRNA reads, dynamic range and, mostly importantly, your discovery potential.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 26, 2017 at 9:30 AM Eastern    Status: Available Reserve
Tuesday, July 18, 2017 at 1:00 PM Eastern    Status: Available Reserve

Introduction to IPA and the powerful knowledge base behind it

Explore how IPA’s knowledge and discovery tools allow you to relate the most recent literature findings to your research and help you in hypothesis generation. Learn how to use IPA to gain detailed information about genes and isoforms and create interactive and customized pathways using tools such as the BioProfiler. You can leverage all of this information instantly without needing to upload any of your data and create interactive and customized pathways using tools such as the BioProfiler.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, July 12, 2017 at 1:00 PM Eastern    Status: Available Reserve

Introduction to the CLC Genomics Workbench: A preview

"This introductory webinar will provide new users with a sneak peek on the basic features of the Genomics Workbench. It will also cover tips and tricks as well as new highlights in the Workbench that will be useful for our more seasoned users. The webinar includes a brief presentation as well as a short live demo with a small NGS dataset. We will be focusing on the following topics: • Introduction to QIAGEN’s bioinformatics portfolio • Overview of the Workbench user interface • Plugins • Customer self-help resources • Running individual tools • Import, QC and pre-processing of NGS data • Import of reference genome data • Export of data • Introduction to Workflows • Batch analysis of data "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, July 19, 2017 at 1:00 PM Eastern    Status: Available Reserve

QIAseq Technologies for Low Input Whole Genome Sequencing

"Rapidly developing next-generation sequencing (NGS) technologies provide highly sensitive methods in discovering and characterizing the genetic information of a variety of samples. However, DNA samples are often limited in quantity, as well as compromised in quality. Such samples are not suitable for standard NGS library construction methods, which commonly require hundreds of nanograms of good-quality DNA. Examples of such challenging clinical samples include circulating DNA, laser capture microdissection (LCM) samples, formalin-fixed paraffin-embedded (FFPE) samples, ancient DNA and chromatin immunoprecipitation (ChIP) samples. In this webinar, we describe the measures that should be taken into consideration while sequencing such challenging samples. We will also present methods that can be used to optimize library construction to efficiently convert small amounts of DNA samples into sequencing libraries, especially for whole genome sequencing applications. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 26, 2017 at 1:00 PM Eastern    Status: Available Reserve
Wednesday, June 28, 2017 at 9:30 AM Eastern    Status: Available Reserve

Digital sequencing: Introduction to Unique Molecular Index (UMI) technology for targeted sequencing

Errors related to the use of PCR in library construction have plagued targeted DNA and RNA sequencing experiments, calling into question the accuracy of results. Digital sequencing overcomes these challenges by introducing Unique Molecular Indices, or UMIs. These molecular barcodes are incorporated into the DNA or RNA sequences before any amplification takes place, eliminating errors such as PCR duplication and amplification bias. This webinar features an in-depth discussion of how UMI technology works and an introduction to how it is used in QIAGEN’s QIAseq sequencing solutions. Find out how to improve your NGS data with the accuracy of UMIs!

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, July 24, 2017 at 1:00 PM Eastern    Status: Available Reserve

Targeted DNA-seq for mutation detection

The utilization of targeted DNA sequencing with enrichment panels is on the rise for detecting genetic variants in cancers, inherited diseases and more. In this webinar, we introduce the QIAseq Targeted DNA Panels, which use Unique Molecular Indices (UMIs) and Single Primer Extension (SPE) technology to improve downstream analysis of difficult-to-sequence genes on Illumina and Thermo Fisher NGS instruments. QIAGEN uses its proprietary knowledge base to design panels for different cancer types, carrier testing, cardiomyopathy and other areas of interest. In addition, QIAGEN offers full coverage of the human genome, allowing researchers to build custom panels for their specific applications. Join us to find out how you can quickly and reliably detect genetic variants in your research from FFPE and fresh samples.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, July 28, 2017 at 1:00 PM Eastern    Status: Available Reserve

Gene expression analysis using targeted RNA-seq and desktop NGS instruments

Not every experiment requires whole transcriptome sequencing, but until recently, there hasn’t been a convenient way to profile transcriptomes in biological research areas such as cancer or immunity. In this webinar, we discuss the strategy and technology of targeted RNA sequencing, including the use of novel targeted RNA-seq panels covering the transcriptomes of 8 different pathways for human and mouse, as well as 170+ smaller panels. Using Single Primer Extension (SPE) and Unique Molecular Index (UMI) technology, the QIAseq panels overcome traditional RNA-seq limitations and ensure increased precision and accuracy. Join us and learn how you can easily upgrade your RNA-seq data and move beyond the limits of qPCR, microarray and traditional RNA-seq.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, July 26, 2017 at 1:00 PM Eastern    Status: Available Reserve

Linking miRNA and gene expression using digital NGS

Total RNA discovery includes exploring both gene expression and regulation. This means analyzing both mRNA and noncoding regulatory RNA species such as microRNA. Until recently, successful miRNA-seq was difficult due to challenges such as contamination with adapter dimers and other RNA species, PCR bias and high sample input requirements. In this webinar, we discuss how new technological advances have helped overcome the challenges of miRNA-seq and RNA-seq, and why NGS is replacing qPCR as the technology of choice for total RNA discovery studies. Join us to find out about the recent advances in RNA sequencing and to learn about a case study in which NGS links microRNA with gene expression analysis.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, July 27, 2017 at 1:00 PM Eastern    Status: Available Reserve

Next-generation sequencing in single-cell applications

Single-cell sequencing is revolutionizing how we understand biology by revealing the individual contributions of cells rather than simply analyzing in bulk. QIAGEN has developed complete cell-to-library solutions for DNA and RNA sequencing libraries from single cells, as well as the novel QIAscout system for reliable single-cell isolation. In this webinar, we discuss several applications for single-cell NGS, along with application data. Find out how to apply NGS to your single-cell research!

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, July 25, 2017 at 1:00 PM Eastern    Status: Available Reserve

Introduction to the CLC Genomics Cloud Engine – Enterprise NGS Made Easy

Reaping the benefits of cloud computing takes more than just moving servers and data online. Ensuring a secure solution and a smooth transition to the cloud is far from trivial, in particular, if results are expected to be identical to an on-premise solution. QIAGEN’s CLC productline is the leading solution for NGS analysis, proven to minimize cost-per-analysis on Intel CPUs. This webinar will introduce you to our newly launched enterprise class cloud solution, the Genomics Cloud Engine.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, June 28, 2017 at 4:00 AM Eastern    Status: Available Reserve
Wednesday, June 28, 2017 at 1:00 PM Eastern    Status: Available Reserve

NGS Live in 30 Days: Challenges of, and a solution to, implementing NGS

Despite decreasing costs and increasing awareness of the important insights it can deliver, many barriers still exist to the broad adoption of next-generation sequencing (NGS) technology by smaller research labs. Obstacles to implementing NGS stemming from its inherent complexity, workflow fragmentation, cost obscurity, and protracted investment of time and resources, are often perceived as too great for many labs to overcome. However, as clinical cancer research expands beyond a small number of large academic centers, the implementation of this technology by all institutions is critical to moving research in precision medicine forward. Here we present the GeneReader NGS System, which has been rigorously tested and successfully implemented by multiple laboratories. Their experience can be used to help other labs considering the NGS technology.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, June 27, 2017 at 4:00 AM Eastern    Status: Available Reserve

Bioinformatics for non-bioinformaticians in cancer clinical research

As its cost and complexity continue to decrease, one of the main bottleneck in NGS lies in bioinformatics analysis and interpretation. Typically, specialized knowledge is required to customize a pipeline, fully understand the sequencing results, and ascribe meaning to variant findings. This is not possible in many laboratories which are battling resource and expertise challenges, while faced with the need to rapidly implement an NGS assay. Here we present the GeneReader NGS System comprising all elements required to lead you from sample preparation to generation of an actionable result. Including fully integrated bioinformatics for analysis and interpretation the workflow was specifically designed for labs of any size to conduct cancer clinical research.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, July 6, 2017 at 4:00 AM Eastern    Status: Available Reserve

NGS: Now integrated and connected with your lab operations

Are you choosing an NGS solution that offers connectivity with the rest of your laboratory operations? Imagine being able to track samples, record reagent lots, store results and manage workflows, all with the help of one easy software interface. In this webinar you will learn how to do this with the QIAGEN GeneRead Link Software.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, July 13, 2017 at 4:00 AM Eastern    Status: Available Reserve

DNA methylation analysis in a single day – the new PyroMark Q48 Autoprep

"Based on sequencing-by-synthesis, Pyrosequencing is a highly flexible technology for rapid and quantitative analysis of any type of sequence variation. The real-time output delivers high-resolution sequence information, making Pyrosequencing highly suitable for various applications, including DNA methylation quantification. This webinar will focus on the following topics - How bisulfite conversion can be improved for more reliable methylation results - How 5-hmC can be differentiated from 5-mC - Why Pyrosequencing is ideally suited for sensitive methylation analysis - What “advanced” Pyrosequencing offers for methylation analysis - How the new PyroMark Q48 Autoprep streamlines the workflow for methylation analysis"

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, July 4, 2017 at 9:30 AM Eastern    Status: Available Reserve

"QIAGEN Wet-Lab Scientist Series: Advice Prior to Using QIAseq Targeted Panels

"QIAseq Targeted Panels incorporate novel Unique Molecular Index (UMI) technology into targeted sequencing, which has allowed scientists to obtain accurate quantitative data and call low-frequency variants. UMIs, together with proprietary QIAGEN library preparation chemistry, enable scientists to sequence regions which were impossible to enrich with targeted approaches in the past. In this 3-part webinar series, we will explore the key points for using QIAseq Targeted Panels and include a series of frequently asked questions, along with tips on how to avoid common pitfalls in order to generate sequencing data of the highest quality. Part one of this series will provide an overview of the key considerations in sample extraction and purification. Many different sample types can be used with our QIAseq Targeted Panels, but we have advice from the laboratory on how to isolate nucleic acids of the highest quality for your NGS experiments. Focus will also be placed on library generation, critical parameters and tips for the wet-bench workflow and quality check procedures to ensure that the library is suitable for sequencing. Example data will be shared to illustrate ideal libraries, and we will also troubleshoot results where the libraries were less than ideal for sequencing. This talk will be hosted by a scientist from our clinical NGS team who has significant wet-bench experience with QIAseq products. We would recommend attending this webinar prior to using QIAseq Targeted Panels. We hope you are able to join us!"

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, July 6, 2017 at 1:00 PM Eastern    Status: Available Reserve

QIAGEN Wet-Lab Scientist Series: Advice for Sequencer Setup & Sequencer Performance Review of QIAseq Targeted Panels

"QIAseq Targeted Panels incorporate novel Unique Molecular Index (UMI) technology into targeted sequencing, which has allowed scientists to obtain accurate quantitative data and call low-frequency variants. UMIs, together with proprietary QIAGEN library preparation chemistry, enable scientists to sequence regions which were impossible to enrich with targeted approaches in the past. In this 3-part webinar series, we will explore the key points for using QIAseq Targeted Panels and include a series of frequently asked questions, along with tips on how to avoid common pitfalls in order to generate sequencing data of the highest quality. Part two of this series will begin with information on how to set up a QIAseq Targeted Panel sequencing run on Illumina or Ion Torrent instruments. We will focus on troubleshooting common mistakes prior to starting the sequencing run. We will also discuss metrics related to post-sequencing and qualification of the run. This can be helpful in troubleshooting and optimization of future runs. This talk will be hosted by a scientist from our clinical NGS team who has significant wet-bench experience with QIAseq products. We would recommend this webinar prior to using QIAseq Targeted Panels. We hope you can join us! "

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, July 13, 2017 at 1:00 PM Eastern    Status: Available Reserve

QIAGEN Wet-Lab Scientist Series: Advice for Data Analysis of QIAseq Targeted Panels

"QIAseq Targeted Panels incorporate novel Unique Molecular Index (UMI) technology into targeted sequencing, which has allowed scientists to obtain accurate quantitative data and call low-frequency variants. UMIs, together with proprietary QIAGEN library preparation chemistry, enable scientists to sequence regions which were impossible to enrich with targeted approaches in the past. In this 3-part webinar series, we will explore the key points for using QIAseq Targeted Panels and include a series of frequently asked questions, along with tips on how to avoid common pitfalls in order to generate sequencing data of the highest quality. Part three of this webinar series will focus on data analysis after the completion of the sequencing run. QIAGEN offers a variety of data analysis options as part of our sample to insight initiative. We will discuss our cloud-based analysis software and provide a demonstration. Next, we will explore QIAGEN Biomedical Workbench and show how this powerful software suite can be used for the analysis of a wide range of NGS datasets. We will also briefly discuss QIAGEN Clinical Insight, which comprises of the Analyze module for reviewing variants and Interpret, which assists in building the final report. This talk will be hosted by a scientist from our clinical NGS team who has significant wet-bench experience with QIAseq products. We would recommend this webinar prior to using QIAseq Targeted Panels. We hope you can join us!"

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, July 20, 2017 at 1:00 PM Eastern    Status: Available Reserve

Algorithms in TB testing at Scripps Mercy Hospital – effectively managing TB Testing results

"The Occupational Health department at Scripps Mercy Hospital in California has developed a new set of tuberculosis testing algorithms to manage TB test results, specifically indeterminate results. These three algorithms have proven to be highly effective to aid Scripps Mercy in routine and post-exposure TB testing using either QuantiFERON-TB Gold or the tuberculin skin test as the primary testing method. Embracing standardized and data-driven protocols has provided increased consistency in TB testing, and use of the algorithms has spread among Occupation Health departments throughout the Scripps organization. Scripps Mercy began using QuantiFERON-TB Gold in 2013, both in their clinics and for new hires. The hospital started performing QFT testing on all employees in July 2016. The Occupational Health department’s decision to switch from TST to QFT-based screening for all employees was driven by many compelling factors, including (1) the higher specificity of the test, (2) the ease of single-visit testing, (3) the cost savings associated with reduced staff time, and (4) the improved test performance in BCG-vaccinated populations. In this webinar, Andrea Hurley, RN, MSN will first present the development and application of Scripps Mercy Hospital’s TB testing algorithms. Assistant Medical Director Dr. Ger will follow with a presentation detailing key factors in Scripps Mercy's decision to switch from TST- to QFTbased testing."

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, July 10, 2017 at 1:00 PM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.