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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

September 2017
Monday
Tuesday
Wednesday
Thursday
Friday

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5

Nucleic acid purification on QIAcube

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Targeted RNA-seq for gene expression

Targeted RNA-seq for gene expression

7

8

Introducing Unique Molecular Indices technology

11

Targeted DNA-seq for mutation detection

12

Genotyping and microbial identification on Rotor-Gene Q

miRNA-seq in liquid biopsy

13

RNAseq data analysis with Biomedical GW

RNAseq data analysis with Biomedical GW

14

gDNA collection, storage, and purification

NGS in single-cell applications

15

Practical hints for successful PCR

Practical hints for successful PCR

18

Circulating biomarker isolation from liquid biopsy samples

19

Sample QC and reproducibility

20

Biologically interpret RNAseq data with IPA

Biologically interpret RNAseq data with IPA

21

gDNA isolation from solid tissue samples

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25

Circulating miRNA analysis with NGS

Single-cell RNA-seq - QIAscout & QIAseq FX Single Cell RNA Library Kit

26

DNA methylation analysis on PyroMark Q48 Autoprep

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Critical factors for successful multiplex qPCR

Critical factors for successful multiplex qPCR

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Current Seminar Titles Available:

Focus Title
1.  Multiplex PCRCritical Factors for Successful Multiplex Real-Time PCR
2.  DNA sample isolationTips and Tricks for Rapid Isolation of Genomic DNA from Solid Tissue Samples
3.  QIAseq and QIAscoutSingle-cell RNA-seq using live cells and microrafts – linking cell morphology to transcriptomics
4.  AutomationDNA methylation analysis in a single day – the new PyroMark Q48 Autoprep
5.  BioinformaticsBiologically interpret your RNA-seq data with knowledge-based IPA
6.  QIAseq NGSCirculating miRNA and RNA identification for biomarker assessment

Critical Factors for Successful Multiplex Real-Time PCR

Multiplex real-time PCR is a powerful tool for gene expression analysis, viral load monitoring, genotyping, and many other applications. The ability to amplify and detect several genomic DNA, cDNA, or RNA targets in the same reaction offers many benefits: • Conservation of precious samples – more quantification data per sample • Increased throughput – more targets analyzed per run on a cycler • Reliable results – no well-to-well variability due to co-amplification of internal control • Reduced costs – save time and reagents The QuantiFast Multiplex PCR and RT-PCR kits are optimized for reliable amplification of many different templates despite a high variability in abundance. Thus they enable successful amplification of multiple targets on the first attempt without optimization. This webinar explains the principles of the QIAGEN multiplex technologies and shows data demonstrating the exceptional multiplex real-time PCR performance of the QuantiFast Multiplex kits.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 28, 2017 at 9:30 AM Eastern    Status: Available Reserve
Thursday, September 28, 2017 at 1:00 PM Eastern    Status: Available Reserve

Tips and Tricks for Rapid Isolation of Genomic DNA from Solid Tissue Samples

Obtaining reliable results from genotyping or sequencing experiments using solid tissue is not always easy. In order to have a successful experiment, you must start with sufficient amounts of high-quality DNA. An inefficient lysis results in poor yields, low DNA integrity and consequently, inferior quality results, thereby limiting the insights you can attain from your sample. In this webinar, we will talk about solutions to overcome the challenges of lysis of fibrous or hard tissue so that you can achieve the insights you value.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, September 21, 2017 at 1:00 PM Eastern    Status: Available Reserve

Single-cell RNA-seq using live cells and microrafts – linking cell morphology to transcriptomics

"Dose-response studies and the analysis of involved genes and pathways is a key application in multiple research areas, especially in drug toxicity and cancer research. Single-cell analysis enables scientists to understand responses of individual cells by providing valuable information about gene expression variability. In this webinar, we will introduce a robust and easy-to-use single-cell RNA-seq workflow for studying cells selected for analysis based on morphological alterations after drug treatment. We will demonstrate an innovative microraft-based technology, the QIAscout, which is used with a standard microscope to isolate live single cells after being treated with PMA (phorbol 12-myristate 13-acetate). Transcriptome data from RNA-seq of responder versus non-responder cells analyzed using the QIAseq FX Single Cell RNA Library Kit will be presented. We will also provide further details on QIAGEN’s single-cell analysis workflow, which offers a simple and ideal platform to perform dose-response and time-course studies based on live cell morphology, allowing scientists to link cell biology to transcriptomics. If you’re working with live eukaryotic cells, join us and learn how you can quickly adapt this workflow to your lab."

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 25, 2017 at 1:00 PM Eastern    Status: Available Reserve

DNA methylation analysis in a single day – the new PyroMark Q48 Autoprep

"Based on the sequencing-by-synthesis principle, Pyrosequencing is a highly flexible technology for rapid and quantitative analysis of any type of sequence variation. The real-time output delivers high-resolution sequence information, making Pyrosequencing highly suitable for various applications, particularly for DNA methylation quantification. Combined with the latest EpiTect Fast bisulfite conversion technology, the new PyroMark Q48 Autoprep can now provide highly automated methylation analysis in a single day. This webinar will focus on the following topics: • How bisulfite conversion can be improved for more reliable methylation results • How 5-hmC can be differentiated from 5-mC • Why Pyrosequencing is ideally suited for sensitive methylation analysis • What does “advanced” Pyrosequencing offer for methylation analysis • How the new PyroMark Q48 Autoprep streamlines the workflow for methylation analysis"

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, September 26, 2017 at 1:00 PM Eastern    Status: Available Reserve

Biologically interpret your RNA-seq data with knowledge-based IPA

"In this webinar, we will show the Expression Analysis results generated from a QIAseq Targeted RNA-seq panel dataset. You can find out how to view and interpret your Expression Analysis results in IPA and learn about the multiple ways of relating the molecules in your dataset to the body of information in the Ingenuity Knowledge Base. Discover more about: • Signaling and metabolic canonical pathways enriched in your data • Biological functions and diseases that are over-represented in your data • Predicted upstream regulators that may explain the expression changes observed in your data • How IPA’s integration of analysis results creates causal hypotheses about how upstream regulators influence downstream phenotypes and biological functions • Interaction networks (algorithmically-generated pathways describing potential molecular interactions in your experimental system) • Molecules and biological processes that are predicted to be activated or inhibited in your experimental system"

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, September 20, 2017 at 9:30 AM Eastern    Status: Available Reserve
Wednesday, September 20, 2017 at 1:00 PM Eastern    Status: Available Reserve

Circulating miRNA and RNA identification for biomarker assessment

"Circulating RNA can serve as a powerful biomarker for patient stratification and assessment of overall health and prognosis. In this webinar, we will focus on using next-generation sequencing (NGS) for identification of miRNA and mRNAs from biofluids, including best practices for designing and executing a successful experiment. While RNA is vulnerable to degradation in serum/plasma due to the abundance of RNAseq and other degrading enzymes, mRNA and miRNA are protected within exosomes and other type of vesicles. Enrichment of exosomes from biofluids provides a supply of RNA biomarkers which can be profiled by NGS and further verified using targeted sequencing or qPCR. In this webinar, we will show how QIAGEN’s QIAseq miRNA Library Kit with Unique Molecular Indexes (UMIs) gives researchers several advantages compared to other small RNA sequencing kits. QIAseq miRNA delivers a gel-free workflow down to 1 ng of total RNA input and its proprietary chemistry minimizes ligation and increases the number of miRNA reads by actively blocking non-relevant, contaminating side products and RNAs. Other kits on the market may include a gel-free workflow or reduced ligation bias, but their protocols and methods fall short on delivering these promises. With the inclusion of UMIs in the QIAseq miRNA Library Kit, researchers are ensured that they have sequenced deep enough to cover the entire library and PCR and sequencing bias and errors are removed. In addition, we will address workflows for exosome mRNA discovery using stranded RNAseq and subsequent verification using targeted RNA-seq. QIAGEN’s exosome RNA discovery pipeline starts with a global view of the expressed RNA which is accurately identified using QIAGEN Biomedical Workbench’s tunable algorithm and verified using QIAseq Targeted RNA Panels which employ UMIs to ensure accurate quantification and adequate sequencing depth for each sample you run. Come listen to the RNA experts discuss the ‘next generation’ of sequencing-based workflows for biomarker detection and verification!"

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, September 25, 2017 at 9:30 AM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.