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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

May 2017
Monday
Tuesday
Wednesday
Thursday
Friday

1

2

3

Introduction to IPA

Introduction to IPA

4

5

8

New QIAamp and QS EZ1 ccfDNA kits

9

QIAscout application

10

Formatting and uploading your data into IPA

Formatting and uploading your data into IPA

11

NGS in hematologic malignancies and solid tumors

12

15

QIAseq library kits for ccfDNA sequencing

16

17

Interpreting the results of your Core Analysis in IPA

Interpreting the results of your Core Analysis in IPA

18

Reproducibility, QC & importance of automation

19

22

ccfDNA workflow on QIAsymphony

Introducing Unique Molecular Indices technology

23

Targeted DNA-seq for mutation detection

24

QIAscout and AdnaTest for CTCs

Targeted RNA-seq for gene expression

25

Linking miRNA & gene expression using digital NGS

26

NGS in single-cell applications

29

30

31

Current Seminar Titles Available:

Focus Title
1.  RNA IsolationBack to Basics: Fundamental Concepts and Special Considerations in RNA Isolation
2.  RNA isolationRNA Integrity and Quality – Standardize RNA Quality Control
3.  DNA MethylationDNA Methylation – an Essential Element in Epigenetics Facts, Technologies and Stories
4.  QIAseq miRNA Library KitIntroduction to miRNA-seq using unique molecular indices and gel-free library construction
5.  QIAseq miRNA Library KitmiRNA-seq from liquid biopsy: robust detection from the lowest sample amounts
6.  CLC Genomics WorkbenchIntroduction to the CLC Genomics Workbench: A preview
7.  CLC Genomics WorkbenchFishing for high confidence variants from NGS data using the CLC Genomics Workbench
8.  CLC Genomics WorkbenchStatistical analysis, visualization and functional enrichment of RNA-seq data in the CLC Genomics Workbench
9.  QIAseq NGS Library KitQIAseq Technologies for Low Input Whole Genome Sequencing
10.  Liquid BiopsiesIsolation and molecular characterization of single CTCs after AdnaTest immunomagnetic pre-enrichment
11.  Liquid BiopsiesLess is more – improved methods for fast and efficient ccfDNA purification using spin-column-based methods
12.  Liquid BiopsiesNGS library prep methods for mutation detection from cell-free DNA
13.  Liquid BiopsiesStandardize and streamline ccfDNA-based liquid biopsy workflows through automation using the QIAsymphony SP
14.  QIAseq NGSDigital sequencing technology for hematologic malignancies and solid tumors
15.  Microbiome Characterizing the microbiome of neonates and infants to explore associations with health and disease

Back to Basics: Fundamental Concepts and Special Considerations in RNA Isolation

How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: • The basic methods and challenges of RNA purification • Special considerations for challenging sample types • Isolating miRNA and extracellular RNA • Performing RNA quality checks Join us to learn how you can apply these practical tips to high quality and quantity of RNA.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, June 22, 2017 at 9:30 AM Eastern    Status: Available Reserve

RNA Integrity and Quality – Standardize RNA Quality Control

RNA integrity and quality are critical to obtain meaningful and reliable downstream data. This webinar discusses the challenges and considerations of handling RNA samples, the need for quality control analysis and common methods for RNA integrity and quality assessment. The QIAxcel Advanced System will be introduced to automate the process of RNA sample integrity analysis and obtain objective quality measurement. Application data will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, June 29, 2017 at 9:30 AM Eastern    Status: Available Reserve

DNA Methylation – an Essential Element in Epigenetics Facts, Technologies and Stories

"DNA methylation forms one of the multiple layers of epigenetic mechanisms controlling and modulating gene expression. It closely interacts with histone modifications and chromatin-remodeling complexes to form the genomic chromatin landscape. DNA methylation is essential for mammalian development, imprinting and plays a role in maintaining genomic stability, as well as in dosage compensation. Aberrant DNA methylation has been detected in a number of diseases, such as cancer, neurodevelopmental diseases or autoimmune diseases. This webinar • Discusses the different forms of cytosine methylation • Explains the role of DNA methylation as a regulator of gene expression • Illustrates the implications of DNA methylation in diseases • Unravels the methods for analyzing DNA methylation"

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, June 27, 2017 at 9:30 AM Eastern    Status: Available Reserve

Introduction to miRNA-seq using unique molecular indices and gel-free library construction

"In this webinar, we will introduce the newest NGS solution from QIAGEN, the QIAseq miRNA Library Kit. miRNA sequencing has the potential to uncover new miRNAs, identify processing intermediates and also quantitative differences between samples. However, challenges such as FFPE and serum/plasma samples, the need for high amounts of sample input and tedious workflows using size selection electrophoresis has led to disappointing and often irreproducible results. QIAGEN has developed a revolutionary new miRNA sequencing kit to remove these limitations for miRNA-seq. Our kit ensures highly reproducible and cost-effective miRNA-seq – regardless of the sample. Whether you’re working with serum, cells, tissues or FFPE samples, proprietary technology provided by the QIAseq miRNA Library Kit ensures removal of adapter-based dimers, which allows you to achieve higher levels of usable miRNA mapped reads. In addition, the kit allows a completely gel-free workflow for maximum convenience, starting with only 1 ng of total RNA. The QIAseq miRNA Library Kit includes a free online data analysis portal for mapping reads and interpreting the unique molecular indices (UMI), allowing you to obtain the most unbiased and highest-fidelity results possible. Join us for this webinar to discover what you’ve been missing in your miRNA-seq experiments. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 19, 2017 at 9:30 AM Eastern    Status: Available Reserve

miRNA-seq from liquid biopsy: robust detection from the lowest sample amounts

miRNAs impact virtually all areas of biology, and in circulation, they are promising biomarker candidates for both normal and disease biology. miRNAs are protected from degradation in virtually all biofluids by exosomes, Ago2, HDL or other protective proteins, but are expressed at low levels. As a result, expression analysis, particularly using next-generation sequencing (NGS), has proven to be difficult. Traditional small RNA library kits lack the sensitivity or specificity to adequately assess miRNA expression. Libraries prepared with using these kits have been fraught with background products, such as adapter dimers and other RNAs, including hY4 Y RNA. These problems collectively manifest as a low mapping percentage to miRNA, a limited dynamic range and lost discovery potential. QIAGEN’s QIAseq miRNA Library Kit is specifically designed to overcome these challenges. The innovative, gel-free workflow enables the preparation of robust libraries from even the most difficult, low RNA content biofluids. QIAseq miRNA maximizes your on-target miRNA reads, dynamic range and, mostly importantly, your discovery potential.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 26, 2017 at 9:30 AM Eastern    Status: Available Reserve

Introduction to the CLC Genomics Workbench: A preview

"This introductory webinar will provide new users with a sneak peek on the basic features of the Genomics Workbench. It will also cover tips and tricks as well as new highlights in the Workbench that will be useful for our more seasoned users. The webinar includes a brief presentation as well as a short live demo with a small NGS dataset. We will be focusing on the following topics: • Introduction to QIAGEN’s bioinformatics portfolio • Overview of the Workbench user interface • Plugins • Customer self-help resources • Running individual tools • Import, QC and pre-processing of NGS data • Import of reference genome data • Export of data • Introduction to Workflows • Batch analysis of data "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, June 7, 2017 at 4:00 AM Eastern    Status: Available Reserve
Wednesday, June 7, 2017 at 1:00 PM Eastern    Status: Available Reserve

Fishing for high confidence variants from NGS data using the CLC Genomics Workbench

"Speaker: Dr. Prakriti Mudvari In this webinar, we will demonstrate how to identify high confidence genetic variants starting from raw sequencing reads in the Genomics Workbench. The session will include a brief presentation as well as a short live demo with a small NGS dataset. We will be covering the topics below during the webinar: • Mapping of raw reads to reference • QC tools • Pipeline for improving confidence of variant detection • Overview of variant detectors • Basic and noise filters • Comparison and annotation of variants • Examining functional consequences of identified variants • Visualization of data "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, June 14, 2017 at 4:00 AM Eastern    Status: Available Reserve
Wednesday, June 14, 2017 at 1:00 PM Eastern    Status: Available Reserve

Statistical analysis, visualization and functional enrichment of RNA-seq data in the CLC Genomics Workbench

"This webinar presents how to analyze RNA-seq data starting from raw sequencing reads in the Genomics Workbench. The session will include a brief presentation as well as a short live demo with a small NGS dataset. We will be covering the topics below during the webinar: • Mapping of reads to the reference and abundance estimation • Principal component analysis (PCA) of RNA-seq data • Statistical analysis of differential expression • Visualization of results using volcano plots and Venn diagrams • Creating RNA-seq expression table and adding GO annotations • Gene set enrichment analysis using hypergeometric test "

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, June 21, 2017 at 4:00 AM Eastern    Status: Available Reserve
Wednesday, June 21, 2017 at 1:00 PM Eastern    Status: Available Reserve

QIAseq Technologies for Low Input Whole Genome Sequencing

"Rapidly developing next-generation sequencing (NGS) technologies provide highly sensitive methods in discovering and characterizing the genetic information of a variety of samples. However, DNA samples are often limited in quantity, as well as compromised in quality. Such samples are not suitable for standard NGS library construction methods, which commonly require hundreds of nanograms of good-quality DNA. Examples of such challenging clinical samples include circulating DNA, laser capture microdissection (LCM) samples, formalin-fixed paraffin-embedded (FFPE) samples, ancient DNA and chromatin immunoprecipitation (ChIP) samples. In this webinar, we describe the measures that should be taken into consideration while sequencing such challenging samples. We will also present methods that can be used to optimize library construction to efficiently convert small amounts of DNA samples into sequencing libraries, especially for whole genome sequencing applications. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 26, 2017 at 1:00 PM Eastern    Status: Available Reserve
Wednesday, June 28, 2017 at 9:30 AM Eastern    Status: Available Reserve

Isolation and molecular characterization of single CTCs after AdnaTest immunomagnetic pre-enrichment

Circulating tumor cells (CTCs) are present in different types, including cytokeratin-positive CTCs, stem cell CTCs, apoptotic CTCs and small CTCs. The ability to isolate single CTCs and characterize their patient-specific expression profile is promising for diagnosis and clinical treatment. In this webinar, Dr. Norbert Hochstein and Dr. Siegfried Hauch introduce the latest development – QIAscout single cell technology and AdnaTest technology, for isolation and molecular characterization of single CTCs. Detailed experimental design and data will be presented to demonstrate that single CTCs that have been pre-enriched with AdnaTest ProstateCancerSelect technology are suitable for isolation using the QIAscout system. Furthermore, the single CTCs isolated with the QIAscout system are compatible with downstream mRNA profiling using AdnaTest ProstateCancerDetect. The combined technologies offer an efficient workflow and a powerful tool for analysis of single CTCs to help elucidate the heterogeneous character of single cells and the analysis of rare cells. Come and learn how you can apply these advanced technologies for your liquid biopsy research.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, June 1, 2017 at 1:00 PM Eastern    Status: Available Reserve

Less is more – improved methods for fast and efficient ccfDNA purification using spin-column-based methods

Cell-free DNA (ccfDNA) originating from malignant tumors or a developing fetus is circulated in plasma, serum and other body fluids. It is present mostly as short fragments of less than 500 bp and in low concentration, approximately 1–100 ng/ml. The fragmented nature and low concentration make the purification of ccfDNA very challenging. QIAGEN’s QIAamp Circulating Nucleic Acid Kit has been the gold standard for ccfDNA isolation. Recently, QIAGEN has developed the next-generation of ccfDNA isolation kits – the QIAamp ccfDNA MinElute Mini/Midi Kits and EZ1 ccfDNA Mini/Midi Kits. These kits provide unmatched efficiency and flexibility in ccfDNA isolation. More importantly, these kits allow you to concentrate purified ccfDNA to ensure the highest sensitivity in your downstream NGS or PCR analysis. Join us and learn about the latest advances and how you can accelerate your ccfDNA research.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 5, 2017 at 11:00 AM Eastern    Status: Available Reserve

NGS library prep methods for mutation detection from cell-free DNA

The analysis of cell-free DNA (ccfDNA) derived from serum or plasma is an established method for mutational screening in cancer diagnosis, prognosis and patient stratification. Liquid biopsy NGS applications offer highly sensitive, efficient and optimized workflows. The ccfDNA concentration in serum or plasma is normally very low, which makes sequencing library construction challenging. In this webinar, we will describe the technical challenges associated with ccfDNA sequencing and outline QIAGEN’s optimized ccfDNA workflow that combines high-efficiency NGS library construction with unbiased library amplification.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 12, 2017 at 11:00 AM Eastern    Status: Available Reserve

Standardize and streamline ccfDNA-based liquid biopsy workflows through automation using the QIAsymphony SP

QIAsymphony protocols and kit solutions allow flexible automation for consistency and ease in cfDNA isolation. The PAXgene Blood ccfDNA System streamlines the complete ccfDNA preanalytical workflow, including blood collection, sample stabilization during transport, plasma generation and automated ccfDNA isolation. Dedicated QIAsymphony protocols appropriately address different target molecule populations. High and low sample input volumes and variable eluate volumes can be used, while the handling features of the primary tube minimize the risk of sample mix-up and reduce processing time and costs. The QIAsymphony Circulating DNA Kit standardizes ccfDNA isolation for regulated workflows and allows ccfDNA isolation from plasma using a variety of commonly used blood collection tubes and ccfDNA isolation from urine. Both QIAsymphony solutions demonstrate high performance in both PCR- and NGS-based downstream assays.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 19, 2017 at 11:00 AM Eastern    Status: Available Reserve

Digital sequencing technology for hematologic malignancies and solid tumors

Cancer is the result of the accumulation of multiple abnormalities over time that can disrupt normal cellular function & allow cells to proliferate unregulated, survive, invade, metastasize. The cause of this unregulated growth can be due to a number of genomic changes, including fusion genes – hybrid genes formed from 2 originally separate genes – and DNA alterations including somatic mutations, SNPs and copy number variations. In this webinar, we will review the history and mechanisms of hematologic malignancies and solid tumors, as well as discussing current detection technologies and new digital NGS technologies for cancer research. We will introduce the new digital sequencing approach, which is based on the unique molecular indices (UMIs) method to provide high specificity, sensitivity and limit PCR duplicates and errors. Detailed workflow and applications in hematologic malignancies and solid tumors will be highlighted. Join us and learn about this exciting new digital sequencing technology.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, June 12, 2017 at 9:30 AM Eastern    Status: Available Reserve

Characterizing the microbiome of neonates and infants to explore associations with health and disease

This webinar will focus on the acquisition and development of the preterm gut microbiome from birth and following discharge from intensive care. Specifically, we will discuss the association of the gut microbiome with necrotizing enterocolitis (NEC) and late onset sepsis (LOS), as well as the impact of birth mode. We will also discuss analysis of multi-omic datasets, including the analysis of the airway microbiome and metabolome in infants hospitalized with bronchiolitis.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, June 15, 2017 at 11:30 AM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.