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Online Seminars

SABiosciences is pleased to provide free, on-line educational resources covering relevant tools and techniques for gene and protein expression analysis, and cell-based gene function analysis. We offer live webinars, prerecorded and PDF formatted presentations.

See the instructions for the Web Seminars

July 2017
Monday
Tuesday
Wednesday
Thursday
Friday

3

4

Pyrosequencing in epigenetic research

5

6

Advice for QIAseq Targeted Panels

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10

QuantiFERON: IHN QFT

11

Introduction to miRNA-seq

12

Introduction to IPA

13

14

17

18

miRNA-seq in liquid biopsy

19

CLC Genomics Workbench: Introduction

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21

24

Introducing Unique Molecular Indices technology

25

NGS in single-cell applications

26

Targeted RNA-seq for gene expression

GeneReader NGS System

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Linking miRNA & gene expression using digital NGS

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Targeted DNA-seq for mutation detection

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Single-cell RNA-seq - QIAscout & QIAseq FX Single Cell RNA Library Kit

Current Seminar Titles Available:

Focus Title
1.  RNA IsolationBack to Basics: Fundamental Concepts and Special Considerations in RNA Isolation
2.  RNA isolationRNA Integrity and Quality – Standardize RNA Quality Control
3.  QIAseq NGSDigital sequencing: Introduction to Unique Molecular Index (UMI) technology for targeted sequencing
4.  QIAseq NGSTargeted DNA-seq for mutation detection
5.  QIAseq NGSGene expression analysis using targeted RNA-seq and desktop NGS instruments
6.  QIAseq NGSLinking miRNA and gene expression using digital NGS
7.  QIAseq NGSNext-generation sequencing in single-cell applications
8.  Liquid BiopsiesLess is more – improved methods for fast and efficient ccfDNA purification using spin-column-based methods
9.  Liquid BiopsiesNGS library prep methods for mutation detection from cell-free DNA
10.  Liquid BiopsiesStandardize and streamline ccfDNA-based liquid biopsy workflows through automation using the QIAsymphony SP
11.  Targeted sequencing in cancer clinical researchTargeted sequencing in cancer clinical research
12.  NGS Live in 30 Days (GeneReader)NGS Live in 30 Days: Challenges of, and a solution to, implementing NGS
13.  Bioinformatics for nonbioinformaticiansBioinformatics for non-bioinformaticians in cancer clinical research
14.  NGS: Now integrated and connected with your lab operationsNGS: Now integrated and connected with your lab operations
15.  QIAseq and QIAscoutSingle-cell RNA-seq using live cells and microrafts – linking cell morphology to transcriptomics
16.  GeneReaderGeneReader NGS System Actionable Insights Delivered Through Medical Data-Guided NGS Panel Design
17.  Liquid BiopsiesIdentification and interpretation of pathogenic variants in cell-free DNA – what are the challenges and how to overcome them

Back to Basics: Fundamental Concepts and Special Considerations in RNA Isolation

How are your RNA yields? Some sample types present special challenges in RNA purification and analysis. In this webinar, we will discuss and provide tips for the following topics: • The basic methods and challenges of RNA purification • Special considerations for challenging sample types • Isolating miRNA and extracellular RNA • Performing RNA quality checks Join us to learn how you can apply these practical tips to high quality and quantity of RNA.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, August 15, 2017 at 1:00 PM Eastern    Status: Available Reserve

RNA Integrity and Quality – Standardize RNA Quality Control

RNA integrity and quality are critical to obtain meaningful and reliable downstream data. This webinar discusses the challenges and considerations of handling RNA samples, the need for quality control analysis and common methods for RNA integrity and quality assessment. The QIAxcel Advanced System will be introduced to automate the process of RNA sample integrity analysis and obtain objective quality measurement. Application data will be presented.

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, August 22, 2017 at 1:00 PM Eastern    Status: Available Reserve

Digital sequencing: Introduction to Unique Molecular Index (UMI) technology for targeted sequencing

Errors related to the use of PCR in library construction have plagued targeted DNA and RNA sequencing experiments, calling into question the accuracy of results. Digital sequencing overcomes these challenges by introducing Unique Molecular Indices, or UMIs. These molecular barcodes are incorporated into the DNA or RNA sequences before any amplification takes place, eliminating errors such as PCR duplication and amplification bias. This webinar features an in-depth discussion of how UMI technology works and an introduction to how it is used in QIAGEN’s QIAseq sequencing solutions. Find out how to improve your NGS data with the accuracy of UMIs!

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, July 24, 2017 at 1:00 PM Eastern    Status: Available Reserve

Targeted DNA-seq for mutation detection

The utilization of targeted DNA sequencing with enrichment panels is on the rise for detecting genetic variants in cancers, inherited diseases and more. In this webinar, we introduce the QIAseq Targeted DNA Panels, which use Unique Molecular Indices (UMIs) and Single Primer Extension (SPE) technology to improve downstream analysis of difficult-to-sequence genes on Illumina and Thermo Fisher NGS instruments. QIAGEN uses its proprietary knowledge base to design panels for different cancer types, carrier testing, cardiomyopathy and other areas of interest. In addition, QIAGEN offers full coverage of the human genome, allowing researchers to build custom panels for their specific applications. Join us to find out how you can quickly and reliably detect genetic variants in your research from FFPE and fresh samples.

Duration: 45 minutes followed by Q&A session.

Schedule:

Friday, July 28, 2017 at 1:00 PM Eastern    Status: Available Reserve

Gene expression analysis using targeted RNA-seq and desktop NGS instruments

Not every experiment requires whole transcriptome sequencing, but until recently, there hasn’t been a convenient way to profile transcriptomes in biological research areas such as cancer or immunity. In this webinar, we discuss the strategy and technology of targeted RNA sequencing, including the use of novel targeted RNA-seq panels covering the transcriptomes of 8 different pathways for human and mouse, as well as 170+ smaller panels. Using Single Primer Extension (SPE) and Unique Molecular Index (UMI) technology, the QIAseq panels overcome traditional RNA-seq limitations and ensure increased precision and accuracy. Join us and learn how you can easily upgrade your RNA-seq data and move beyond the limits of qPCR, microarray and traditional RNA-seq.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, July 26, 2017 at 1:00 PM Eastern    Status: Available Reserve

Linking miRNA and gene expression using digital NGS

Total RNA discovery includes exploring both gene expression and regulation. This means analyzing both mRNA and noncoding regulatory RNA species such as microRNA. Until recently, successful miRNA-seq was difficult due to challenges such as contamination with adapter dimers and other RNA species, PCR bias and high sample input requirements. In this webinar, we discuss how new technological advances have helped overcome the challenges of miRNA-seq and RNA-seq, and why NGS is replacing qPCR as the technology of choice for total RNA discovery studies. Join us to find out about the recent advances in RNA sequencing and to learn about a case study in which NGS links microRNA with gene expression analysis.

Duration: 45 minutes followed by Q&A session.

Schedule:

Thursday, July 27, 2017 at 1:00 PM Eastern    Status: Available Reserve

Next-generation sequencing in single-cell applications

Single-cell sequencing is revolutionizing how we understand biology by revealing the individual contributions of cells rather than simply analyzing in bulk. QIAGEN has developed complete cell-to-library solutions for DNA and RNA sequencing libraries from single cells, as well as the novel QIAscout system for reliable single-cell isolation. In this webinar, we discuss several applications for single-cell NGS, along with application data. Find out how to apply NGS to your single-cell research!

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, July 25, 2017 at 1:00 PM Eastern    Status: Available Reserve

Less is more – improved methods for fast and efficient ccfDNA purification using spin-column-based methods

Cell-free DNA (ccfDNA) originating from malignant tumors or a developing fetus is circulated in plasma, serum and other body fluids. It is present mostly as short fragments of less than 500 bp and in low concentration, approximately 1–100 ng/ml. The fragmented nature and low concentration make the purification of ccfDNA very challenging. QIAGEN’s QIAamp Circulating Nucleic Acid Kit has been the gold standard for ccfDNA isolation. Recently, QIAGEN has developed the next-generation of ccfDNA isolation kits – the QIAamp ccfDNA MinElute Mini/Midi Kits and EZ1 ccfDNA Mini/Midi Kits. These kits provide unmatched efficiency and flexibility in ccfDNA isolation. More importantly, these kits allow you to concentrate purified ccfDNA to ensure the highest sensitivity in your downstream NGS or PCR analysis. Join us and learn about the latest advances and how you can accelerate your ccfDNA research.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, August 7, 2017 at 9:30 AM Eastern    Status: Available Reserve

NGS library prep methods for mutation detection from cell-free DNA

The analysis of cell-free DNA (ccfDNA) derived from serum or plasma is an established method for mutational screening in cancer diagnosis, prognosis and patient stratification. Liquid biopsy NGS applications offer highly sensitive, efficient and optimized workflows. The ccfDNA concentration in serum or plasma is normally very low, which makes sequencing library construction challenging. In this webinar, we will describe the technical challenges associated with ccfDNA sequencing and outline QIAGEN’s optimized ccfDNA workflow that combines high-efficiency NGS library construction with unbiased library amplification.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, August 14, 2017 at 9:30 AM Eastern    Status: Available Reserve

Standardize and streamline ccfDNA-based liquid biopsy workflows through automation using the QIAsymphony SP

QIAsymphony protocols and kit solutions allow flexible automation for consistency and ease in cfDNA isolation. The PAXgene Blood ccfDNA System streamlines the complete ccfDNA preanalytical workflow, including blood collection, sample stabilization during transport, plasma generation and automated ccfDNA isolation. Dedicated QIAsymphony protocols appropriately address different target molecule populations. High and low sample input volumes and variable eluate volumes can be used, while the handling features of the primary tube minimize the risk of sample mix-up and reduce processing time and costs. The QIAsymphony Circulating DNA Kit standardizes ccfDNA isolation for regulated workflows and allows ccfDNA isolation from plasma using a variety of commonly used blood collection tubes and ccfDNA isolation from urine. Both QIAsymphony solutions demonstrate high performance in both PCR- and NGS-based downstream assays.

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, August 21, 2017 at 9:30 AM Eastern    Status: Available Reserve

Targeted sequencing in cancer clinical research

In recent years, rapid advances in cancer genetics have revealed the importance of mutations in tumor cell proliferation and in determining the likely effectiveness of cancer therapies. As such, many cancer research laboratories recognize the value of interrogating the cancer genome in order to further understand the underlying molecular mechanisms driving tumorigenesis. In order to achieve this, labs are turning to Next-Generation Sequencing (NGS) to provide answers that will aid in the fight against cancer . Targeted sequencing using cancer-specific panels offers an efficient and effective way to capture the most relevant information. In this webinar you will learn how to apply current NGS technologies efficiently and effectively in cancer clinical research.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, August 2, 2017 at 4:00 AM Eastern    Status: Available Reserve

NGS Live in 30 Days: Challenges of, and a solution to, implementing NGS

Despite decreasing costs and increasing awareness of the important insights it can deliver, many barriers still exist to the broad adoption of next-generation sequencing (NGS) technology by smaller research labs. Obstacles to implementing NGS stemming from its inherent complexity, workflow fragmentation, cost obscurity, and protracted investment of time and resources, are often perceived as too great for many labs to overcome. However, as clinical cancer research expands beyond a small number of large academic centers, the implementation of this technology by all institutions is critical to moving research in precision medicine forward. Here we present the GeneReader NGS System, which has been rigorously tested and successfully implemented by multiple laboratories. Their experience can be used to help other labs considering the NGS technology.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, August 9, 2017 at 4:00 AM Eastern    Status: Available Reserve

Bioinformatics for non-bioinformaticians in cancer clinical research

As its cost and complexity continue to decrease, one of the main bottleneck in NGS lies in bioinformatics analysis and interpretation. Typically, specialized knowledge is required to customize a pipeline, fully understand the sequencing results, and ascribe meaning to variant findings. This is not possible in many laboratories which are battling resource and expertise challenges, while faced with the need to rapidly implement an NGS assay. Here we present the GeneReader NGS System comprising all elements required to lead you from sample preparation to generation of an actionable result. Including fully integrated bioinformatics for analysis and interpretation the workflow was specifically designed for labs of any size to conduct cancer clinical research.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, August 23, 2017 at 4:00 AM Eastern    Status: Available Reserve

NGS: Now integrated and connected with your lab operations

Are you choosing an NGS solution that offers connectivity with the rest of your laboratory operations? Imagine being able to track samples, record reagent lots, store results and manage workflows, all with the help of one easy software interface. In this webinar you will learn how to do this with the QIAGEN GeneRead Link Software.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, August 16, 2017 at 4:00 AM Eastern    Status: Available Reserve

Single-cell RNA-seq using live cells and microrafts – linking cell morphology to transcriptomics

"Dose-response studies and the analysis of involved genes and pathways is a key application in multiple research areas, especially in drug toxicity and cancer research. Single-cell analysis enables scientists to understand responses of individual cells by providing valuable information about gene expression variability. In this webinar, we will introduce a robust and easy-to-use single-cell RNA-seq workflow for studying cells selected for analysis based on morphological alterations after drug treatment. We will demonstrate an innovative microraft-based technology, the QIAscout, which is used with a standard microscope to isolate live single cells after being treated with PMA (phorbol 12-myristate 13-acetate). Transcriptome data from RNA-seq of responder versus non-responder cells analyzed using the QIAseq FX Single Cell RNA Library Kit will be presented. We will also provide further details on QIAGEN’s single-cell analysis workflow, which offers a simple and ideal platform to perform dose-response and time-course studies based on live cell morphology, allowing scientists to link cell biology to transcriptomics. If you’re working with live eukaryotic cells, join us and learn how you can quickly adapt this workflow to your lab."

Duration: 45 minutes followed by Q&A session.

Schedule:

Monday, July 31, 2017 at 9:30 AM Eastern    Status: Available Reserve

GeneReader NGS System Actionable Insights Delivered Through Medical Data-Guided NGS Panel Design

In this webinar, Dr. Yassmine Akkari, Ph.D., FACMG is going to share her experience in NGS panel-based cancer clinical testing, and some of the challenges. She is also going to speak to the QIAGEN Actionable Insights Panel design process, and how this unique process led to robust content focused on variants of clinical actionability.

Duration: 45 minutes followed by Q&A session.

Schedule:

Wednesday, July 26, 2017 at 11:00 PM Eastern    Status: Available Reserve

Identification and interpretation of pathogenic variants in cell-free DNA – what are the challenges and how to overcome them

"Several studies have shown that the analysis of cfDNA enables the detection of pathogenic variants, which can be important for treatment decisions. The use of cfDNA has many advantages as it does not require surgery. Moreover, it may help in the detection of early tumor recurrence and upcoming resistance. However, due to dilution from non-cancerous cells, variants are expected at a very low allele percentage, which makes it very hard to detect them and to separate them from false positives. In addition, the interpretation of data in terms of being able to identify pathogenic variants can be challenging as well. In this webinar, we will show how cfDNA data from next-generation sequencing can be analyzed with a high level of accuracy. In a case study, we will compare variants identified in cfDNA with those identified in the buffy coat and tumor, and show potential new candidates. "

Duration: 45 minutes followed by Q&A session.

Schedule:

Tuesday, August 29, 2017 at 9:30 AM Eastern    Status: Available Reserve

Note: Viewers will be asked to register before viewing the previously recorded webinars.