qBiomarker™ Somatic Mutation Assay for : SMPH010463A

Product Catalog #	337011
Assay Catalog #	SMPH010463A
Species	Human
Gene Symbol	PDGFRA
RefSeq Accession #	NM_006206
Nucleotide Change
Amino Acid Change	p.M844_S847del
COSMIC ID	12402
Also Detect	12402
Assay Kit Description	qBiomarker Somatic Mutation Assay & Reference Assay for: Human PDGFRA mutation , p.M844_S847del (COSMIC ID: 12402)

Description

The qBiomarker Somatic Mutation PCR Assays are a fast and reliable mutation analysis tool using ARMS® (Amplification Refractory Mutation System) primer-based allele discrimination and Hydrolysis Probe-based quantitative real-time PCR technology. It utilizes a proprietary and experimentally verified algorithm for designing mutation-specific qPCR primers and probe. Each qBiomarker Somatic Mutation PCR Assay is subjected to rigorous experimental verification. Both sensitive detection of the intended mutation in a sample (as low as 1% mutant sample on a wild-type sample background) and assay specificity (i.e. discrimination between mutant sample and wild-type sample) are guaranteed when used with the qBiomarker Probe Master Mix. A gene-specific reference assay for checking sample quality and measuring sample input and gene copy dosage is included for each mutation assay. See the qBiomarker Somatic Mutation PCR Array System User Manual/ Handbook for protocol to follow. The qBiomarker Somatic Mutation PCR Assays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Materials Included / Packing List

Please check the kit components immediately after you receive this package. SABiosciences is only responsible for missing items reported within two (2) business days of receipt. One tube of qBiomarker™ Somatic Mutation Assay for for 100 reactions One tube of qBiomarker Somatic Mutation PCR Reference Assay for Human PDGFRA for 100 reactions. Two tubes of qBiomarker Probe Master Mix (1.25 mL each), either: SM-051F : qBiomarker Probe Master Mix / Fluorescein SM-052R: qBiomarker Probe Master Mix / ROX

Shipping & Storage Conditions

Somatic Mutation PCR Assays Shipping Conditions: PCR Assays are shipped on Blue Ice (BI). Storage Conditions: Keep assays at -20 ºC for long-term storage. qBiomarker Probe Master Mixes Shipping Conditions: qBiomarker Probe Master Mixes are shipped on Blue Ice (BI). Storage Conditions: Keep qBiomarker Probe Master Mixes at 4 ºC for long-term storage. NOTE: Be sure that you have the correct Master Mix (with correct Reference Dye) for your instrument before starting the experiment. For instruments that do not require a reference dye, please use the qBiomarker Probe / ROX Master Mix.

Shelf Life: All reagents are stable for 6 months after receipt of the kit if stored at the recommended temperature.

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