SureENTRY™ Transduction Reagent

The SureENTRY™ Transduction Reagent enhances lentiviral/retroviral transductions 10-fold to 1000-fold in most mammalian cell types, with minimal toxicity. The success of an experiment involving lentiviral delivery technology is often dependent upon the efficiency with which the genetic payload is delivered into the target cells. Including SureENTRY Transduction Reagent in your transduction protocol dramatically improves the efficiency of lentiviral/retroviral delivery. The SureENTRY Transduction Reagent is a valuable accessory product for any experiment using Cignal Lenti Reporters.

Product Listing

Product Name	Cat #	Unit
SureENTRY Transduction Reagent	SA-02	0.5 ml (enough for 12,500 transductions or 130 96-well plates)

Why Use SureENTRY Transduction Reagent?

• High Efficiency Transduction
  SureENTRY Transduction Reagent has been shown to enhance lentiviral transduction 10-fold to 1000-fold, depending upon the target cell
• Sterile Cell Culture Grade Reagent
  Ready to use aqueous solution simplifies your lentiviral transduction protocols
• Optimized Formulation
  No need to screen numerous transduction reagent formulations

HOW IT WORKS

APPLICATIONS DATA

• Study cell signaling in Primary Cells, Stem Cells, and Difficult to Transfect Cell Lines

Cignal Lenti NFAT reporter determines PKA/Ca 2+ pathway activity in human primary cells (Normal Human Pulmonary Artery Smooth Muscle Cells; PASMC): Cignal Lenti NFAT reporter (luc) [4X10⁵ TU] and Cignal Lenti Renilla control (luc) [1X10⁵ TU] co-transduced around 10,000 PASMC cells in the presence of 8 µg/ml SureENTRY Transduction Reagent (24 hours before transduction 5,000 cells were plated per well of 96-well plate). After 48 hours of transduction, medium was changed to assay medium (Opti-MEM + 0.5% FBS + 0.1mM NEAA + 1mM Sodium pyruvate + 100 U/ml penicillin + 100 µg/ml streptomycin). After 54 hours of transduction, cells were treated with 10 ng/ml PMA and 0.5 µM ionomycin for 18 hours. Dual-luciferase assay was performed, and promoter activity values are expressed as arbitrary units using a Renilla reporter for internal normalization. Experiments were done in triplicates, and the standard deviation is indicated.

Cignal Lenti NFkB reporter measures NFkB pathway activity in thymocytic cells (D1; Murine T-Cell Leukemia Cells): Cignal Lenti NFΚB reporter (luc) [2.5X10⁵ TU] transduced around 10,000 D1 cells, an IL-7-dependent murine thymocyte cell, in the presence of 8 µg/ml SureENTRY Transduction Reagent (24 hours before transduction 5,000 cells were plated per well of 96-well plate). After 48 hours of transduction, cells were treated with 20 ng/ml of recombinant mouse tumor necrosis factor alpha (hTNFα) protein for 18 hours. Luciferase assay was performed, and promoter activity values are expressed as arbitrary units. Experiments were done in triplicates, and the standard deviation is indicated.